I have over 30 years experience in electron microscopy (TEM and SEM), light microscopy and their preparation techniques. My major interests are in confocal microscopy with particular emphasis on live cell imaging.
- Live cell imaging
- Super resolution
- High Content Screening
- TEM & SEM
My research interests are in applying microscopy to the study live cells across a range of disciplines, with a particular experience in confocal microscopy. I have recently been appointed to lead the School of Life Sciences imaging platform (SLIM) with a dedicated team of 5 imaging specialists. We cover and have expertise in wide field, confocal, super resolution, HCS, TIRF, EM, histology and image processing and analysis.
I have published in a number of journals, for example Development, Developmental Biology, Nature, Nature Neuroscience, Science, Current Biology, Journal of Pathology, British Journal of Pharmocology, Journal of Clinical Investigation and Molecular Therapy.
- Zeiss 710 confocal microscope
- Two Zeiss 880 confocals
- Zeiss NLO META Combi 2 Photon confocal microscope
- Zeiss LSM Exciter confocal with Wide field
- Zeiss TIRF3
- Deltavision Elite
- MDC confocal and widefield imaging plate readers
- Leica SP2
I am an Associate Fellow of the HEA and run regular workshops for DTP post graduate students, undergraduate student practical classes and lecture on the MSC Stem cell course.
Expertise in Confocal and live cell imaging, electron and light microscopy, cell culture.
SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24 KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
ORIHUELA, C.J., MAHDAVI, J., THORNTON, J., MANN, B., WOOLDRIDGE, K.G., ABOUSEADA, N., OLDFIELD, N.J., SELF, T., ALA'ALDEEN, D.A.A. and TUOMANEN, E.I., 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119(6), 1638-1646