School of Life Sciences
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Tim Self

Head of SLIM facility, Faculty of Medicine & Health Sciences

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Expertise Summary

I have over 30 years experience in electron microscopy (TEM and SEM), light microscopy and their preparation techniques. My major interests are in confocal microscopy with particular emphasis on live cell imaging.

Research Techniques/Expertise

  • Confocal
  • TIRF
  • Live cell imaging
  • Super resolution
  • High Content Screening
  • TEM & SEM
  • Immunocytochemistry
  • Cell culture

Interests

My research interests are in applying microscopy to the study live cells across a range of disciplines, with a particular experience in confocal microscopy. I have recently been appointed to lead the School of Life Sciences imaging platform (SLIM) with a dedicated team of 5 imaging specialists. We cover and have expertise in wide field, confocal, super resolution, HCS, TIRF, EM, histology and image processing and analysis.

Publications

I have published in a number of journals, for example Development, Developmental Biology, Nature, Nature Neuroscience, Science, Current Biology, Journal of Pathology, British Journal of Pharmocology, Journal of Clinical Investigation and Molecular Therapy.

Equipment/ systems

  • Zeiss 710 confocal microscope
  • Two Zeiss 880 confocals
  • Zeiss NLO META Combi 2 Photon confocal microscope
  • Zeiss LSM Exciter confocal with Wide field
  • Zeiss TIRF3
  • Deltavision Elite
  • MDC confocal and widefield imaging plate readers
  • Leica SP2

Teaching Summary

I am an Associate Fellow of the HEA and run regular workshops for DTP post graduate students, undergraduate student practical classes and lecture on the MSC Stem cell course.

Research Summary

Expertise in Confocal and live cell imaging, electron and light microscopy, cell culture.

Selected Publications

Future Research

I am in the process of developing a High Content Screening facility incorporating MDC confocal and widefield plate readers, a plate loading robot and image analysis and archiving system.

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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