School of Life Sciences
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Tim Self

Chief Experimental Officer - Imaging, Faculty of Medicine & Health Sciences

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Expertise Summary

I have over 20years experience in electron microscopy (TEM and SEM), light microscopy and their preparation techniques. My major interests are in confocal microscopy with particular emphasis on live cell imaging.

Research Techniques/Expertise

  • Confocal
  • FRAP, FLIP,FRET
  • Live cell confocal imaging
  • High Content Screening
  • TEM & SEM
  • Immunocytochemistry
  • Cell culture

Interests

My main interests are using confocal microscopy to study membrane receptors. Over recent years I have studied the trafficking and cycling of GFP tagged-receptors using both live and fixed cells. Future directions lie in developing existing confocal systems and the development of our high content screening platforms.

Publications

I have published in a number of journals, for example Development, Developmental Biology, Nature, Nature Neuroscience, Science, Current Biology, Journal of Pathology, British Journal of Pharmocology, Journal of Clinical Investigation and Molecular Therapy.

Equipment/ systems

  • Zeiss 710 confocal microscope
  • Zeiss LSM510uv META combi confocal microscope
  • Zeiss NLO META Combi 2 Photon confocal microscope
  • Zeiss LSm Exciter confocal with TIRF
  • Zeiss Confocor FCS microscope
  • MDC confocal and widefield imaging plate readers
  • MDC Flexstation

Research Summary

Expertise in Confocal and live cell imaging, electron and light microscopy, cell culture.

Selected Publications

Future Research

I am in the process of developing a High Content Screening facility incorporating MDC confocal and widefield plate readers, a plate loading robot and image analysis and archiving system.

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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