I have over 20years experience in electron microscopy (TEM and SEM), light microscopy and their preparation techniques. My major interests are in confocal microscopy with particular emphasis on live cell imaging.
- FRAP, FLIP,FRET
- Live cell confocal imaging
- High Content Screening
- TEM & SEM
My main interests are using confocal microscopy to study membrane receptors. Over recent years I have studied the trafficking and cycling of GFP tagged-receptors using both live and fixed cells. Future directions lie in developing existing confocal systems and the development of our high content screening platforms.
I have published in a number of journals, for example Development, Developmental Biology, Nature, Nature Neuroscience, Science, Current Biology, Journal of Pathology, British Journal of Pharmocology, Journal of Clinical Investigation and Molecular Therapy.
- Zeiss 710 confocal microscope
- Zeiss LSM510uv META combi confocal microscope
- Zeiss NLO META Combi 2 Photon confocal microscope
- Zeiss LSm Exciter confocal with TIRF
- Zeiss Confocor FCS microscope
- MDC confocal and widefield imaging plate readers
- MDC Flexstation
Expertise in Confocal and live cell imaging, electron and light microscopy, cell culture.
SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24 KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
ORIHUELA, C.J., MAHDAVI, J., THORNTON, J., MANN, B., WOOLDRIDGE, K.G., ABOUSEADA, N., OLDFIELD, N.J., SELF, T., ALA'ALDEEN, D.A.A. and TUOMANEN, E.I., 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119(6), 1638-1646