Pyocins as alternatives to antibiotics against Pseudomonas aeruginosa infections
Fact file
| Duration |
Three to four years full-time |
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| Eligibility |
Self-funded; Home, EU or International Students |
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| Supervisor(s) |
Dr Christopher Penfold |
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| Application deadline |
No deadline |
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About the project
Pyocins are bacteriocins produced by Pseudomonas aeruginosa to kill closely related strains of the same species during times of environmental stress. There are three types of pyocin: (i) type R and (ii) F pyocins resemble phage particles in structure and kill bacteria by depolarisation of the cytoplasmic membrane, (iii) S type pyocins proteins that kill sensitive bacteria by degrading their DNA. Recently, we have used PCR to determine the distribution of pyocin types within laboratory strains and clinical isolates of Pseudomonas aeruginosa. Using biological activity assays and DNA mutagenesis we have defined the sensitivity and resistance profiles of all isolates against other isolates and have shown that inhibition of growth of one isolate by another isolate is related to pyocin type. In this project, we will determine the role of pyocins in shaping interactions of different isolates of Pseudomonas aeruginosa in liquid culture and biofilms. Pseudomonas aeruginosa is very capable of forming biofilms as a means of cell to cell communication and protection of individual cells against environmental hazards. Pseudomonas aeruginosa exists in the lungs of sufferers of cystic fibrosis as organised biofilm structures which hinder antibiotic therapies. We will test the impact of pyocins on the stability of individual cells within the biofilm structure and assess the opportunities for pyocin therapy against Pseudomonas aeruginosa in biofilm.
Funding notes
This project is available to self-funded students. Home applicants should contact the supervisor to determine the current funding status for this project. EU applicants should visit the Graduate School webpages for information on specific EU scholarships. International applicants should visit our International Research Scholarships page for information regarding fees and funding at the University.
See this project on FindaPhD
Pyocins as alternatives to antibiotics against Pseudomonas aeruginosa infections