Contact
Biography
I am a molecular biologist and lead of the Gene Regulation & RNA Biology Laboratory. Following a PhD at NUI, Galway, Ireland working on bacterial gene regulation, I worked with Pierre Chambon & Regine Losson at the IGBMC Strasbourg, France (1990-1995) supported by fellowship awards from EMBO, ARC and Univ. Louis Pasteur. My research investigated Nuclear Receptor activation functions and set up yeast two-hybrid approaches to identify transcriptional cofactors (e.g. Heery et al, PNAS 1993; Heery et al, NAR 1994; Pierrat et al, Gene 1994; LeDouarin et al, EMBO J 1995; vom Baur et al, EMBO J 1996). I then joined Malcolm Parker's group at Imperial Cancer Research, London as a Marie Curie Fellow, where a major advance from my research was the identification of the LXXLL motifs that mediate NR/cofactor interactions (e.g. Heery et al Nature 1997; Kalkhoven et al, EMBO J; Bevan et al, Mol Cell Biol 1999). I was awarded a Wellcome Trust Senior Fellowship in Basic Biomedical Sciences to establish a group at Biochemistry, U. Leicester (1998-2006). We continued to characterise the structure function of NRs and cofactors, (e.g. Heery et al, JBC 2001; Sheppard et al, Mol Cell Biol 2001; Coulthard et al, JBC 2003; Waters et al JBC 2006; Ryan et al Cell Cycle 2006) but also the function of MOZ/KAT6A histone acetyltransferase in oncogeneic fusions in AML (Kindle et al Mol Cell Biol 1995; Collins et al, JBC 1996). We have continued these research themes at the School of Pharmacy Nottingham (e.g. Kindle et al, Leukemia 2010; Chan et al NAR 2013, Wadelin et al PLOS One 2013; Dreveny et al NAR 2014; Fulton et al, CDDis 2017; Garvin et al NAR 2019).
We enjoy a wide network of collaborators at Nottingham (Paula Moran; Nigel Mongan; Anna Piccinini, Simon Johnston; Alan McIntyre, Cameron Alexander, Pavel Gershkovich; Franco Falcone); and internationally (Jenny Persson, Umea; Lukas Kenner, Vienna).
Expertise Summary
Molecular & Cell Biology
CRISPR CAS9 Genome Editing
Protein-Protein & Protein-DNA Interactions
Nuclear Receptor Signaling; Transcription Factors & Coactivators
Histone Modifications; Chromatin Recognition, Oncogenic Fusion Proteins
Cancer- Leukemia; Prostate Cancer; Breast Cancer
Confocal Microscopy, Cell-based Assays, RNA Seq, Chromatin -IP,
Protein Expression for Structure/ Function Studies
MYST Acetyltransferases
PIP5K1A Function
Teaching Summary
I teach on a number of modules across several of the degree courses offered by the School of Pharmacy, the details of which are outlined below. The topics covered fall largely within my research… read more
Research Summary
Prof. David Heery's research team is housed within the Gene Regulation & RNA Biology Laboratory (GRRB) located in the Psychology/Pharmacy Building Science Road, Main Campus. Our research is… read more
Selected Publications
KAUR, I., SCHRAMM, G., EVERTS, B., SCHOLZEN, T., KINDLE, K.B., BEETZ, C., MONTIEL-DUARTE, C., BLINDOW, S., JONES, A.T., HAAS, H., STOLNIK, S., HEERY, D.M. and FALCONE, F.H., 2011. Interleukin-4 inducing principle from Schistosoma mansoni eggs contains a functional C-terminal nuclear localization signal necessary for nuclear translocation in mammalian cells but not for its uptake Infection and Immunity. 79(4), 1779-1788 RYAN, COLM M, KINDLE, KARIN B, COLLINS, HILARY M and HEERY, DAVID M, 2010. SUMOylation regulates the nuclear mobility of CREB binding protein and its association with nuclear bodies in live cells. Biochemical and biophysical research communications. 391(1), 1136-41 KINDLE, K.B., COLLINS, H.M. and HEERY, D.M., 2010. MOZ-TIF2-mediated destruction of CBP/p300 is blocked by calpain inhibitor 2 Leukemia. 24(7), 1359-1361 CANNELL, I.G., KONG, Y.W., JOHNSTON, S.J., CHEN, M.L., COLLINS, H.M., DOBBYN, H.C., ELIA, A., KRESS, T.R., DICKENS, M., CLEMENS, M.J., HEERY, D.M., GAESTEL, M., EILERS, M., WILLIS, A.E. and BUSHELL, M., 2010. p38 MAPK/MK2-mediated induction of miR-34c following DNA damage prevents Myc-dependent DNA replication Proceedings of the National Academy of Sciences of the United States of America. 107(12), 5375-5380
Current Group Members
Prof. David M Heery - Group Leader
Dr. Hilary M Collins -Senior Experimental Officer/ formerly CRUK PDRA
Christopher Roberts BBSRC DTP
Mitchell Masterson BBSRC DTP (with Prof PM Moran)
Jonas Schoenfeld MRes
Ryan Morris BBSRC DTP
Ruby Chrisp BBSRC DTP (with Prof PM Moran)
Barbara Rampersad Technician
ALUMNI from the DMH Lab
Dr Jonathan Whitchurch BBSRC DTP
Dr Cintia Monteiro CAPES Science without Borders
Dr. Bismoy Mazumder Vice Chancellor Scholarship
Dr. Miguel Hernandez MRes
Dr Maria Clemente MRes
Dr Amit Bhattacharrya MRes
Dr Neetu Bharti MRes
Dr. Joel Fulton - RPSGB scholar; CRUK Research Associate
Dr. Chun Chan Leukemia Research Gordon Pillar scholarship
Olaide Oyegbami PhD Student (with Prof PM Moran)
Dr Nikolaos Tertipis - MSc student
Dr. Sian Deeves - BBSRC scholarship
Dr. Magdy K Abdelghany PhD Scholarship (Egyptian Govt.)
Dr. Baigong Yue MRC Postdoctoral Fellow
Dr. Frances Wadelin Leukaemia Research- Clinical Training Fellow
Dr. Marie Messmer CRUK Postdoctoral Fellow
Dr. Karin Kindle LLR/AICR/Wellcome Trust Postdoctoral Fellow
Dr. Cristina Montiel-Duarte LLR/AICR Postdoctoral Fellow
Dr. Colm Ryan BBSRC Studentship
Dr. Chun Ming Chan LLR Gordon Piller PhD Studentship
Dr. Cecilie Bay-Richter PhD Student (with Prof P Moran)
Dr. Nijjareeya Sirisriro PhD student
Dr. Maria Viskaduraki LLR Postdoctoral Fellow
Hao Liu MSc student
Dr. Lorna Waters BBSRC PhD Student (with Dr Mark Carr)
Dr. Sachiko Matsuda Wellcome Trust RA/ PhD Student
Dr. Janet Bramham Wellcome Trust Postdoctoral Fellow
Dr. Victoria Coulthard Wellcome Prize PhD Student
Dr. Sagair Hussain MSc Student
Dr. Phil Troke MRC PhD scholarship
Dr. Hilary Sheppard Wellcome Trust Postdoctoral Fellow
Ms. Jan Harries Wellcome Trust Research Assistant
I teach on a number of modules across several of the degree courses offered by the School of Pharmacy, the details of which are outlined below. The topics covered fall largely within my research expertise, including recombinant DNA technology, gene regulation, endocrinology and cancer, thus enabling research-led teaching.
TEACHING & MODULE MANAGEMENT
Degree: M.Pharm. Pharmacy
PHAR2004 B32RED Renal & Endocrine Disease: Module Convenor
PHAR2001 B32GIL GI & Liver Disorders
PHAR4020 B34PLM Professional Leadership & Management
PHAR3005 B33RPJ Research Projects
PHAR1006 B31PRO /B31PC1 Professional Competencies
PHAR2008 B32PRO /B32PC2 Professional Competencies
PHAR3004 B33PRO /B33PC3 Professional Competencies
PHAR4022 B34PRO /B34PC4 Professional Competencies
Degree : BSc International Pharmacy
PHAR2027 B32RAE Renal & Endocrine Disease: Module Convenor
PHAR2025 B32GLD GI & Liver Disorders
Degree: M.Pharm Sci
PHAR1019 B31EAM Endocrine & Metabolism
PG Training Module:
'Introduction to Gene Regulation'
Current Research
Prof. David Heery's research team is housed within the Gene Regulation & RNA Biology Laboratory (GRRB) located in the Psychology/Pharmacy Building Science Road, Main Campus. Our research is focussed on molecular mechanisms in gene regulation, chromatin modification and cell signalling pathways, in the context of cancer and other human diseases. Our lab infrastructure has facilities for bacterial, yeast and mammalian cell culture, biochemistry, molecular and cell biology and confocal microscopy. My team has expertise in protein, DNA and RNA analysis, molecular cloning, bacterial expression, RT-qPCR, enzyme and reporter assays, chromatin IP, transcription profiling, histone modifications, protein-protein and protein-nucleic acid interactions including yeast two-hybrid, GST pulldown, co-immunoprecipitations, EMSA and FRET.
CURRENT PROJECTS:
STRUCTURE / FUNCTION OF NUCLEAR RECEPTOR COMPLEXES
I have a long-standing interest in transcription factors, and in particular the Nuclear Receptor family of ligand regulated TFs. We have investigated protein-DNA and protein-protein interactions of NRs, identifying novel heterodimer complexes and discovering short conserved motifs such as the LXXLL in NR coactivators (Heery et al., PNAS 1992; Heery et al NAR 1994; Heery et al., Nature 1997; Heery et al JBC 2001; Kalkhoven et al., 1998 EMBO J; Bevan et al., Mol Cell Biol 1999; Coulthard et al JBC 2003; Matsuda et al., JBC 2004;Waters et al., JBC 2006; Chan et al NAR 2013; Fulton et al, Cell Death Disease 2017). Recent efforts have focussed on androgen receptor function in collaboration with Prof Lukas Kenner at the Ludwig Boltzmann Institute for Cancer Research, Vienna (Aksoy et al., 2019 submitted).
HISTONE ACETYLTRANSFERASES IN CANCER AND DEVELOPMENTAL DISORDERS
Another major interest is how 'Epigenetic regulators' engage with histones and chromatin to regulate gene expression. Having studied NR coactivators such as RIP140, SRC1, CBP, p300 and BCL11A, we are currently focussed on investigating the structure and function of the MYST acetyltransferase proteins MOZ and MORF that are encoded by the KAT6A and KAT6B genes. These are important for the development of blood, brain and other tissues and function as chromatin regulators, and we have previously studied their role as oncogenic fusion proteins in leukemia (Kindle et al., 2005 MCB; Collins et al., 2006 JBC; Kindle et al., 2010 Leukaemia 2010).
Our Breakthrough paper published in Nucleic Acids Research showed that the Double PHD finger domain recognises specific modifications of lysines and arginines in the Histone H3 tail. Moreover, crystal structures of the DPF in complex with acetylated H3 peptides revealed for the first time that chromatin regulators can induce helical structure in Histone N-terminal tails (Dreveny, Deeves et al, NAR 2014). We are currently using CRISPR CAS9 genome editing and RNA Seq to identify MOZ gene targets and characterise MOZ functional domains important for gene transcription and leukemogenesis, or to recapitulate mutations associated with KAT6A global developmental delay syndrome.
UNDERSTANDING GENE FUNCTION THROUGH GENOME EDITING
We have a number of ongoing collaborations involving CRISPR CAS9 editing of selected genes in cancer cell lines including with Dr Alan McIntyre (BET proteins in Breast Cancer- funded by Breast Cancer Now); Prof Nigel Mongan (KDM6A- funded by Prostate Cancer UK; and METTL3 function in prostate cancer); Dr. Anna Piccinini (TNC gene in macrophages); Prof Paula Moran (circadian regulation by CLOCK proteins). We are also using genome editing and RNA Seq to study the role of PIP2 signalling in prostate cancer with a view to understanding how it intersects with Androgen Receptor function. This is part of a wider collaborative effort to identify novel targets in prostate cancer in collaboration with Prof. J Persson Umea, Sweden and others (Miftakhova et al., Cancer Research 2016; Sarwar et al., Oncotarget 2016).
OTHER PROJECTS:
Other recent studies include the characterisation of human cell nucleus infiltration by proteins secreted from schistosome eggs with Franco Falcone (Kaur et al., Infection & Immunity, 2014; Pennington et al., Infection & Immunity, 2017) , functional analysis of AR mutations in DSD patients with Prof Ieuan Hughes, Cambridge (Lek et al., EBioMedicine 2019 and circadian histone acetylation and dopamine receptorswith Prof Paula Moran (O'Callaghan et al. J. Psychopharm 2014; Oyegbami et al., Current Alzheimers Research 2017). A new collaboration with Dr Simon Johnston (NRF fellow) and colleagues will investigate FOXA1 mutations in invasive lobular breast cancer using RIME analysis of human tumours (funded by AMS).
EXTERNAL FUNDING:
Our research activities have been funded by grants, fellowships and studentships awarded by the Wellcome Trust Cancer Research UK, MRC, Leukaemia Lymphoma Research, AICR, BBSRC, RPSGB, Breast Cancer Now and Prostate Cancer UK.
GARVIN AJ, KHALAF AHA, RETTINO A, XICLUNA J, BUTLER L, MORRIS JR, HEERY DM and CLARKE NM, 2019. GSK3β-SCFFBXW7α mediated phosphorylation and ubiquitination of IRF1 are required for its transcription-dependent turnover. Nucleic acids research. 47(9), 4476-4494 LEE JB, ZGAIR A, MALEC J, KIM TH, KIM MG, ALI J, QIN C, FENG W, CHIANG M, GAO X, VORONIN G, GARCES AE, LAU CL, CHAN TH, HUME A, MCINTOSH TM, SOUKARIEH F, AL-HAYALI M, CIPOLLA E, COLLINS HM, HEERY DM, SHIN BS, YOO SD, KAGAN L, STOCKS MJ, BRADSHAW TD, FISCHER PM and GERSHKOVICH P, 2018. Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system. Journal of controlled release : official journal of the Controlled Release Society. 286, 10-19 LEK, NGEE, TADOKORO-CUCCARO, RIEKO, WHITCHURCH, JONATHAN B., MAZUMDER, BISMOY, MILES, HARRIET, PRENTICE, PHILIPPA, BUNCH, TREVOR, ZIELINSKA, KAROLINA, METZLER, VERONIKA, MONGAN, NIGEL P., HEERY, DAVID M. and HUGHES, IEUAN A., 2018. Predicting puberty in partial androgen insensitivity syndrome: Use of clinical and functional androgen receptor indices EBIOMEDICINE. 36, 401-409 METZLER VM, DE BROT S, ROBINSON RS, JEYAPALAN JN, RAKHA E, WALTON T, GARDNER DS, LUND EF, WHITCHURCH J, HAIGH D, LOCHRAY JM, ROBINSON BD, ALLEGRUCCI C, FRAY RG, PERSSON JL, ØDUM N, MIFTAKHOVA RR, RIZVANOV AA, HUGHES IA, TADOKORO-CUCCARO R, HEERY DM, RUTLAND CS and MONGAN NP, 2017. Androgen dependent mechanisms of pro-angiogenic networks in placental and tumor development. Placenta. S0143-4004(17), 30164-9 FULTON J, MAZUMDER B, WHITCHURCH JB, MONTEIRO CJ, COLLINS HM, CHAN CM, CLEMENTE MP, HERNANDEZ-QUILES M, STEWART EA, AMOAKU WM, MORAN PM, MONGAN NP, PERSSON JL, ALI S and HEERY DM, 2017. Heterodimers of photoreceptor-specific nuclear receptor (PNR/NR2E3) and peroxisome proliferator-activated receptor-γ (PPARγ) are disrupted by retinal disease-associated mutations. Cell death & disease. 8(3), e2677 OYEGBAMI O, COLLINS HM, PARDON MC, EBLING FJ, HEERY DM and MORAN PM, 2017. Abnormal clock gene expression and locomotor activity rhythms in two month-old female APPSwe/PS1dE9 mice. Current Alzheimer research. 14, 850-860 ATIOMO W, SHAFIEE MN, CHAPMAN C, METZLER VM, ABOUZEID J, LATIF A, CHADWICK A, KITSON S, SIVALINGAM VN, STRATFORD IJ, RUTLAND CS, PERSSON JL, ØDUM N, FUENTES-UTRILLIA P, JEYAPALAN JN, HEERY DM, CROSBIE EJ and MONGAN NP, 2017. Expression of NAD(P)H quinone dehydrogenase 1 (NQO1) is increased in the endometrium of women with endometrial cancer and women with Polycystic Ovary Syndrome. Clinical endocrinology. MIFTAKHOVA R, HEDBLOM A, SEMENAS J, ROBINSON B, SIMOULIS A, MALM J, RIZVANOV A, HEERY DM, MONGAN NP, MAITLAND NJ, ALLEGRUCCI C and PERSSON JL, 2016. Cyclin A1 and P450 aromatase promote metastatic homing and growth of stem-like prostate cancer cells in the bone marrow Cancer Research. 76(8), 2453-64 SARWAR M, SEMENAS J, MIFTAKHOVA R, SIMOULIS A, ROBINSON BD, WINGREN AG, MONGAN NP, HEERY DM, JOHNSSON H, ABRAHAMSSON PA, DIZEYI N, LUO J and PERSSON JL, 2016. Targeted suppression of AR-V7 using PIP5K1α inhibitor overcomes enzalutamide resistance in prostate cancer cells. Oncotarget. (In Press.)
DE BROT S, NTEKIM A, CARDENAS R, JAMES V, ALLEGRUCCI C, HEERY D, BATES DO, ODUM N, PERSSON JL and MONGAN NP, 2015. Regulation of vascular endothelial growth factor (VEGF) in prostate cancer. Endocrine-related cancer. 22(3), R107-23 NILSSON EM, LAURSEN KB, WHITCHURCH J, MCWILLIAM A, ODUM N, PERSON JL, HEERY DM, GUDAS LJ and MONGAN NP, 2015. MIR137 is an androgen regulated repressor of an extended network of transcriptional coregulators Oncotarget. 6(34), 35710-25 CHOONG SS, FULTON J,, EMES RD, YON L, HEERY DM and MONGAN NP, 2015. Retinoids: Nutritional, Cellular and Pharmacological roles of the Vitamin A derivatives Reference Module in Biomedical Sciences.
DREVENY, I., DEEVES, S.E., FULTON, J., YUE, B., MESSMER, M., BHATTACHARYA, A., COLLINS, H.M. and HEERY, D.M., 2014. The double PHD finger domain of MOZ/MYST3 induces α-helical structure of the histone H3 tail to facilitate acetylation and methylation sampling and modification Nucleic Acids Research. 42(2), 822-835 COLLINS HM, ABDELGHANY MK, MESSMER M, YUE B, DEEVES SE, KINDLE KB, MANTELINGU K, ASLAM A, WINKLER GS, KUNDU TK and HEERY DM, 2013. Differential effects of garcinol and curcumin on histone and p53 modifications in tumour cells. BMC cancer. 13(1), 37 BAY-RICHTER, C., O'CALLAGHAN, M.J., MATHUR, N., O'TUATHAIGH, C.M.P., HEERY, D.M., FONE, K.C.F, WADDINGTON, J.L. and MORAN, P.M., 2013. D-amphetamine and antipsychotic drug effects on latent inhibition in mice lacking dopamine D2 receptors Neuropsychopharmacology. 38(8), 1512-1520 CHAN, C.M., FULTON, J., MONTIEL-DUARTE, C., COLLINS, H.M., BHARTI, N., WADELIN, F.R., MORAN, P.M., MONGAN, N.P. and HEERY, D.M., 2013. A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors Nucleic Acids Research. 41(21), 9663-9679 KAUR, I., SCHRAMM, G., EVERTS, B., SCHOLZEN, T., KINDLE, K.B., BEETZ, C., MONTIEL-DUARTE, C., BLINDOW, S., JONES, A.T., HAAS, H., STOLNIK, S., HEERY, D.M. and FALCONE, F.H., 2011. Interleukin-4 inducing principle from Schistosoma mansoni eggs contains a functional C-terminal nuclear localization signal necessary for nuclear translocation in mammalian cells but not for its uptake Infection and Immunity. 79(4), 1779-1788 RYAN, COLM M, KINDLE, KARIN B, COLLINS, HILARY M and HEERY, DAVID M, 2010. SUMOylation regulates the nuclear mobility of CREB binding protein and its association with nuclear bodies in live cells. Biochemical and biophysical research communications. 391(1), 1136-41 KINDLE, K.B., COLLINS, H.M. and HEERY, D.M., 2010. MOZ-TIF2-mediated destruction of CBP/p300 is blocked by calpain inhibitor 2 Leukemia. 24(7), 1359-1361 CANNELL, I.G., KONG, Y.W., JOHNSTON, S.J., CHEN, M.L., COLLINS, H.M., DOBBYN, H.C., ELIA, A., KRESS, T.R., DICKENS, M., CLEMENS, M.J., HEERY, D.M., GAESTEL, M., EILERS, M., WILLIS, A.E. and BUSHELL, M., 2010. p38 MAPK/MK2-mediated induction of miR-34c following DNA damage prevents Myc-dependent DNA replication Proceedings of the National Academy of Sciences of the United States of America. 107(12), 5375-5380 WADELIN, F., FULTON, J., MCEWAN, P.A., SPRIGGS, K.A., EMSLEY, J. and HEERY, D.M., 2010. Leucine-rich repeat protein PRAME: expression, potential functions and clinical implications for leukaemia Molecular Cancer. 27(9), 226 ELSHEIKH, S.E., GREEN, A.R., RAKHA, E.A., POWE, D.G., AHMED, R.A., COLLINS, H.M., SORIA, D., GARIBALDI, J.M., PAISH, C.E., AMMAR, A.A., GRAINGE, M.J., BALL, G.R., ABDELGHANY, M.K., MARTINEZ-POMARES, L., HEERY, D.M. and ELLIS, I.O., 2009. Global histone modifications in breast cancer correlate with tumor phenotypes, prognostic factors, and patient outcome Cancer Research. 69(9), 3802-3809 BAY-RICHTER, C., O'TUATHAIGH, C.M.P., O'SULLIVAN, G., HEERY, D.M., WADDINGTON, J.L. and MORAN, P.M., 2009. Enhanced latent inhibition in dopamine receptor- deficient mice is sex-specific for the D1 but not D2 receptor subtype: implications for antipsychotic drug action International Journal of Neuropsychopharmacology. 12(3), 403-414 CRISTIAN BELLODI, KARIN KINDLE, FRANCESCA BERNASSOLA, ANDREA COSSARIZZA,DAVID DINSDALE, GERRY MELINO, DAVID M. HEERY AND PAOLO SALOMONI, 2007. A cytoplasmic PML mutant inhibits p53 function. Cell Cycle. 5(22), 2688-2692 EL-SHEIKH, S., GREEN, A.R., RAKHA, E.A., AHMED, R.A., PAIS, E.C., GRAINGE, M.J., BALL, G.R., ROBERTSON, J.F., HEERY, D.M. and ELLIS, I.O., 2007. Global histone modifications in breast cancer tissue correlate with tumor phenotype, prognostic factors and patient outcome EJC Supplements. 5(3), 14-14 EL-SHEIKH, S.E., GREEN, A.R., RAKHA, E.A., PAISH, E.C., HEERY, D.M. and ELLIS, I.O., 2007. Global histone modifications in breast cancer and their prognostic significance Modern Pathology. 20, 29A-30A
LOPEZ-GARCI, J, THOMAS R, PERIYASAMY M and CHRISTIAN M, LEAO M, JAT P, KINDLE KB, HEERY DM, PARKER MG, BULUWELA L, KAMALATI T & ALI S, 2007. ZNF366 is a Novel Transcriptional Co-repressor that Regulates Estrogen Receptor Activity Through the Recruitment of CtBP and Histone Deacetylases. Nucleic Acids Research. 33(19), 6393-6404
EL-SHEIKH, S.E., GREEN, A.R., RAKHA, E.A., PAISH, E.C., HEERY, D.M. and ELLIS, I.O., 2007. Global histone modifications in breast cancer and their prognostic significance Laboratory Investigation. 87, 29A-30A
FAHEY, A.J., ROBINS, R.A., KINDLE, K.B., HEERY, D.M. and CONSTANTINESCU, C.S., 2006. Effects of glucocorticoids on STAT4 activation in human T cells are stimulus-dependent Journal of Leukocyte Biology. 80(1), 133-144 COLLINS, H.M., KINDLE, K.B., MATSUDA, S., RYAN, C., TROKE, P.J.F., KALKHOVEN, E. and HEERY, D.M., 2006. MOZ-TIF2 alters cofactor recruitment and histone modification at the RARβ2 promoter: differential effects of MOZ fusion proteins on CBP- and MOZ-dependent activators Journal of Biological Chemistry. 281(25), 17124-17133 RYAN, COLM M, HARRIES, JANET C, KINDLE, KARIN B, COLLINS, HILARY M and HEERY, DAVID M, 2006. Functional interaction of CREB binding protein (CBP) with nuclear transport proteins and modulation by HDAC inhibitors. Cell Cycle. 5(18), 2146-52
TROKE, PHILIP J F, KINDLE, KARIN B, COLLINS, HILARY M and HEERY, DAVID M, 2006. MOZ fusion proteins in acute myeloid leukaemia. Biochemical Society symposium.. 23-39
BELLODI, CRISTIAN, KINDLE, KARIN, BERNASSOLA, FRANCESCA, DINSDALE, DAVID, COSSARIZZA, ANDREA, MELINO, GERRY, HEERY, DAVID and SALOMONI, PAOLO, 2006. Cytoplasmic function of mutant promyelocytic leukemia (PML) and PML-retinoic acid receptor-alpha. Journal of Biological Chemistry. 281(20), 14465-73 WATERS, L., YUE, B., VEVERKA, V., RENSHAW, P., BRAMHAM, J., MATSUDA, S., FRENKIEL, T., KELLY, G., MUSKETT, F., CARR, M. and HEERY, D.M., 2006. Structural diversity in p160/CREB-binding protein coactivator complexes Journal of Biological Chemistry. 281(21), 14787-95 GREEN, A.R., EL-SHEIKH, S., RAKHA, E.A., PAISH, E.C., HEERY, D.M. and ELLIS, I.O., 2006. Global histone modifications in breast cancer and their prognostic significance Breast Cancer Research. 8, S16-S16 BELLODI, C., KINDLE, K.B., BERNASSOLA, F., DINSDALE, D., COSSARIZZA, A., MELINO, G., HEERY, D.M. and SALOMONI, P., 2006. Cytoplasmic PML proteins form novel cytoplasmic bodies and inhibit p53- and retinoic acid-dependent transcription. Journal of Biological Chemistry. 281, 14465-14473 (In Press.)
EL-SHEIKH, S., RAKHA, E.A., PAISH, E.C., HEERY, D.M. and SALOMONI, P., 2006. Global histone modifications in breast cancer and their prognostic significance Journal of Pathology. 210, 1-1
BELLODI, C., KINDLE, K., BERNASSOLA, F., DINSDALE, D., COSSARIZZA, A., MELINO, G., HEERY, D. and SALOMONI, P., 2006. Cytoplasmic function of mutant PML and PML-RARalpha British Journal of Haematology. 133, 27-27
BELLODI, C., KINDLE, K., BERNASSOLA, F., DINSDALE, D., COSSARIZZA, A., MELINO, G., HEERY, D. and SALOMONI, P., 2006. Role of cytoplasmic forms of PML and PML-RARa in the response to therapy TOXICOLOGY -SHANNON-. VOL 226(NUMBER 1), 46-47
KINDLE, K.B., TROKE, P.J.F., COLLINS, H.M., MATSUDA, S., BOSSI, D., BELLODI, C., KALKHOVEN, E., SALOMONI, P., PELICCI, P.G., MINUCCI, S. and HEERY, D.M., 2005. MOZ-TIF2 inhibits transcription by nuclear receptors and p53 by impairment of CBP function Molecular and Cellular Biology. 25(3), 988-1002 LUCEY, MARIE J, CHEN, DONGSHENG, LOPEZ-GARCIA, JORGE, HART, STEPHEN M, PHOENIX, FLADIA, AL-JEHANI, RAJAI, ALAO, JOHN P, WHITE, ROGER, KINDLE, KARIN B, LOSSON, RéGINE, CHAMBON, PIERRE, PARKER, MALCOLM G, SCHäR, PRIMO, HEERY, DAVID M, BULUWELA, LAKJAYA and ALI, SIMAK, 2005. T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif. Nucleic Acids Research. 33(19), 6393-404 MATSUDA, S., HARRIES, J.C., VISKADURAKI, M., TROKE, P.J.F., KINDLE, K.B., RYAN, C. and HEERY, D.M., 2004. A conserved α-helical motif mediates the binding of diverse nuclear proteins to the SRC1 interaction domain of CBP Journal of Biological Chemistry. 279(14), 14055-14064 BELLODI, C., KINDLE, K., BERNASSOLA, F., DINSDALE, D., MELINO, G., COSSARIZZA, A., HEERY, D. and SALOMONI, P., 2004. Mutant PML Proteins Form Novel Cytoplasmic Bodies. (Poster # 764-III) BLOOD -NEW YORK-. VOL 104(NUMB 11), 3494
SHEPPARD, H M, MATSUDA, S, HARRIES, J C, KINDLE, K B and HEERY, D M, 2003. Transcriptional activation by estrogen receptor (ERalpha) and steroid receptor coactivator (SRC1) involves distinct mechanisms in yeast and mammalian cells. Journal of Molecular Endocrinology. 30(3), 411-22 COULTHARD, V. H., MATSUDA, S. and HEERY, D. M., 2003. An Extended LXXLL Motif Sequence Determines the Nuclear Receptor Binding Specificity of TRAP220 Journal of Biological Chemistry. VOL 278(PART 13), 10942-10951 GIRAUD, SANDRINE, BIENVENU, FRéDéRIC, AVRIL, SYLVIE, GASCAN, HUGUES, HEERY, DAVID M and COQUERET, OLIVIER, 2002. Functional interaction of STAT3 transcription factor with the coactivator NcoA/SRC1a. Journal of Biological Chemistry. 277(10), 8004-11 HEERY, D.M., HOARE, S., HUSSAIN, S., PARKER, M.G. and SHEPPARD, H., 2001. Core LXXLL motif sequences in CREB-binding protein, SRC1, and RIP140 define affinity and selectivity for steroid and retinoid receptors Journal of Biological Chemistry. 276(9), 6695-6702 SHEPPARD, H.M., HARRIES, J.C., HUSSAIN, S., BEVAN, C. and HEERY, D.M., 2001. Analysis of the steroid receptor coactivator 1 (SRC1)-CREB binding protein interaction interface and its importance for the function of SRC1 Molecular and Cellular Biology. 21(1), 39-50 LE DOUARIN, B, HEERY, D M, GAUDON, C, VOM BAUR, E and LOSSON, R, 2001. Yeast two-hybrid screening for proteins that interact with nuclear hormone receptors. Methods in Molecular Biology. 176, 227-48
BEVAN, C.L., CLAESSENS, F., HOARE, S., HEERY, D.M. and PARKER, M.G., 1999. The AF1 and AF2 domains of the Androgen Receptor interact with distinct regions of SRC1. Molecular & Cellular Biology. 19, 8383-8392
WHITE, R., MAK, H-Y., HEERY, D.M., KALKHOVEN, E., VALENTINE, J., BEVAN, C. and PARKER, M.G., 1999. Coactivators and hormone action European Journal of Cancer. 35, 468 LE DOUARIN, B., VOM BAUR, E., ZECHEL,C., HEERY, D.M., HEINE, M., VIVAT, V., GRONEMEYER, H., LOSSON, R. and CHAMBON, P., 1996. Ligand-dependent interaction of nuclear receptors with potential transcriptional intermediary factors (mediators) Philosophical Transactions of the Royal Society of London. (Part B). 351, 569-578 PARKER, M.G., KALKHOVEN, E., WHITE, R., HENTTU, P., SJOBERG, P., HEERY, D.M. and , 1996. Transcriptional activation by oestrogen receptors Immunology. 89, SP57-SP57
L'HORSET.F., DAUVOIS. S., HEERY. D.M., CAVAILLES. V. and PARKER. M.G., 1996. RIP140 interacts with multiple nuclear receptors by means of two distinct sites Molelcular & Cellular Biology. 16, 6029-6036 (In Press.)
VOM BAUR, E., ZECHEL, C., HEERY, D.M., HEINE, M., GARNIER, J.M., VIVAT, V., LE DOUARIN, B., GRONEMEYER, H., CHAMBON, P. and LOSSON, R., 1996. Differential ligand-dependent interactions between the AF2 activating domain of nuclear receptors and the putative transcriptional intermediary factors mSUG1 and TIF1 EMBO Journal. 15, 110-124
LE DOUARIN, B., ZECHEL, C., GARNIER, J.M., LUTZ, Y., TORA, L., PIERRAT, B., HEERY, D.M., GRONEMEYER, H., CHAMBON, P. and LOSSON, R., 1995. The N-terminal part of TIF1, a Putative Mediator of the Ligand-Dependent Activation Function (AF-2) of Nuclear Receptors, is Fused to B-Raf in the Oncogenic Protein T18 EMBO Journal. 14, 2020-2033 HEERY, D.M., PIERRAT, B., GRONEMEYER, H., CHAMBON, P. and LOSSON, R., 1994. Homo- and Heterodimers of the Retinoid X Receptor (RXR) Activate Transcription in Yeast Nucleic Acids Research. 22, 726-731 FITZPATRICK, R., O'DONAHUE, M., JOY, J., HEERY, D.M. and DUNICAN, L.K., 1994. Construction and Characterisation of recA Deficient Strains of Corynebacterium glutamicum and Brevibacterium lactofermentum Journal of Applied Microbiology & Biotechnology. 42, 575-580 HEERY, D.M., ZACHAREWSKI, T., PIERRAT, B., GRONEMEYER, H. and CHAMBON, P., 1993. Efficient Transactivation by Retinoic Acid Receptors in Yeast requires Retinoid X Receptors Proceedings of the National Academy of Sciences of the United States of America. 90, 4281-4285 GILBERT, D.M., HEERY, D.M., LOSSON, R., LEMOINE, Y. and CHAMBON, P., 1993. Estradiol-Inducible Squelching and Cell Growth Arrest by a Chimeric VP-16-Estrogen Receptor Expressed in Saccharomyces cerevisiae:suppression by Allele of PDR1 Molecular and Cellular Biology. 13, 462-472 HEERY, D.M. and DUNICAN, L.K., 1993. Cloning of the trp Gene Cluster from a Tryptophan-Hyperproducing Strain of Corynebacterium glutamicum: Identification of a Mutation in the trp Leader Sequence. Applied and Environmental Microbiology. 59, 791-799
HINEY, M., DAWSON, M., HEERY, D.M., SMITH, P., GANNON, F. and POWELL, R., 1992. A DNA probe for aeromonas salmonicida Applied & Environmental Microbiology. 58, 1039-1042
BARRY,S., GEARY, S., HANNIFY, C., MACGEARAILT, M., HEERY, D.M., GANNON, F. and POWELL, R., 1992. Rapid Mini-Preparations of Total RNA from Bacteria Nucleic Acids Research. 20, 4940 HEERY, D.M. and DUNICAN, L.K., 1990. Nucleotide Sequence of the Corynebacterium glutamicum trpE Gene Nucleic Acids Research. 23, 7138
POWELL, R., HEERY, D.M., GANNON, F. and DUNICAN, L.K., 1990. Electrotransfection of Escherichia coli with λgt10 DNA FEMS Microbiology Letters. 72, 225-228 POWELL, R., HEERY, D.M., DAWSON, M., DUNICAN, L.K. and GANNON, F., 1990. Skimmed Milk as an Alternative to X-Gal for Visual Discrimination of Background in λgt11 Libraries Nucleic Acids Research. 18, 2190 HEERY, D.M., GANNON, F. and POWELL, R., 1990. A Simple Method for Subcloning DNA Fragments from Gel Slices Trends in Genetics. 6, 173
HEERY, D.M, and DUNICAN, L.K., 1989. Improved M13 Cloning using Electroporation Nucleic Acids Research. 17, 8006 HEERY, D.M., POWELL, R., GANNON, F. and DUNICAN, L.K., 1989. Curing of a Plasmid from E.coli using High Voltage Electroporation Nucleic Acids Research. 17, 13031 NEILAN, J., HEERY, D.M. and DUNICAN, L.K., 1985. Isolation of Repliation Loci of Plasmid Origin from Rhizobium trifolii Biochemical Sociey Transactions. 14, 478