Ed Bolt

Contact

• workRoom D55 and D58 The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30194
• fax0115 82 30142
• ed.bolt@nottingham.ac.uk
• http://www.nottingham.ac.uk/life-sciences/people/ed.bolt

Biography

University of London, UCL, St Barts & QMUL: BSc. & PhD

Wellcome Trust Research Career development Fellowship, University of Nottingham 2002-2006

Present (2023): Professor of Molecular Biology

Service on advisory boards, impact and policy committees, and funding panels for UKRI, charity and industrial/drug discovery bodies in The UK and internationally

Fellowship of HEA

Expertise Summary

Homologous recombination in DNA repair

CRISPR biology (prokaryotes)

CRISPR biotechnology

Teaching Summary

I convene lecture and practical modules about molecular biology of DNA repair, CRISPR systems and RNA biology.

Fellow of HEA

Research Summary

Further information can be seen in Orcid; https://orcid.org/0000-0002-5656-7706

and ResearchGate: https://www.researchgate.net/profile/Ed-Bolt

Selected Publications

• IVANA IVANCIC-BACE, SIMON D CASS, STEPHEN J WEARNE and EDWARD L BOLT, 2015. Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research.
• ROLLIE, CLARE, SCHNEIDER, STEFANIE, BRINKMANN, ANNA SOPHIE, BOLT, EDWARD L and WHITE, MALCOLM F, 2015. Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife. 4,
• CASS SD, HAAS KA, STOLL B, ALKHNBASHI O, SHARMA K, URLAUB H, BACKOFEN R, MARCHFELDER A and BOLT EL, 2015. The role of Cas8 in type I CRISPR interference BioScience Reports. 35(3), e00197
• GONG B, SHIN M, SUN J, JUNG CH, BOLT EL, VAN DER OOST J and KIM JS, 2014. Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proc Natl Acad Sci U S A.

• View all publications

Past Research

Please search PubMed for 'Edward_Bolt'

• BUCKLEY R, KRAMM K, COOPER CDO, GROHMANN D and BOLT EL, 2020. Mechanistic insights into Lhr helicase function in DNA repair. The Biochemical journal. 477(16), 2935-2947
• SEYS FM, ROWE P, BOLT EL, HUMPHREYS CM and MINTON NP, 2020. A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences. Genes. 11(4),
• HE L, ST JOHN JAMES M, RADOVCIC M, IVANCIC-BACE I and BOLT EL, 2020. Cas3 Protein-A Review of a Multi-Tasking Machine. Genes. 11(2),
• CHUN HANG LAU and EDWARD BOLT, 2020. Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9 Bioscience Reports.
• LAU CH, REEVES R and BOLT EL, 2019. Adaptation processes that build CRISPR immunity: creative destruction, updated. Essays in biochemistry. 63(2), 227-235
• KILLELEA T, HAWKINS M, HOWARD JL, MCGLYNN P and BOLT EL, 2018. DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase. RNA biology. PMID: 30096986
• RADOVCIC M, KILLELEA T, SAVITSKAYA E, WETTSTEIN L, BOLT EL and IVANCIC-BACE I, 2018. CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases. Nucleic acids research. PMID: 30189098
• CUBBON A, IVANCIC-BACE I and BOLT EL, 2018. CRISPR-Cas immunity, DNA repair and genome stability. Bioscience reports. 38(5), PMID: 30209206
• NORTHALL, S., BUCKLEY, R., JONES, N., PENEDO, C., SOULTANAS, P. and BOLT, E., 2017. DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain. DNA Repair (Amst). 57, 125-132
• MAJSEC K, BOLT EL and IVANČIĆ-BAĆE I, 2016. Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS. BMC microbiology. 16, 28
• NORTHALL SJ, IVANČIĆ-BAĆE I, SOULTANAS P and BOLT EL, 2016. Remodeling and Control of Homologous Recombination by DNA Helicases and Translocases that Target Recombinases and Synapsis. Genes. 7(8),
• BOLT, EDWARD L., JENKINS, TABITHA, RUSSO, VALERIA MOREIRA, AHMED, SHARLENE, CAVEY, JAMES and CASS, SIMON D., 2016. Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair BIOSCIENCE REPORTS. 36,
• CASS SD, HAAS KA, STOLL B, ALKHNBASHI O, SHARMA K, URLAUB H, BACKOFEN R, MARCHFELDER A and BOLT EL, 2015. The role of Cas8 in type I CRISPR interference BioScience Reports. 35(3), e00197
• ROLLIE, CLARE, SCHNEIDER, STEFANIE, BRINKMANN, ANNA SOPHIE, BOLT, EDWARD L and WHITE, MALCOLM F, 2015. Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife. 4,
• IVANA IVANCIC-BACE, SIMON D CASS, STEPHEN J WEARNE and EDWARD L BOLT, 2015. Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research.
• GONG B, SHIN M, SUN J, JUNG CH, BOLT EL, VAN DER OOST J and KIM JS, 2014. Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proceedings of the National Academy of Sciences of the United States of America. 111(46), 16359-64
• IVANČIĆ-BAĆE, I., RADOVČIĆ, M., BOČKOR, L., HOWARD, J.L. and BOLT, E.L., 2013. Cas3 stimulates runaway replication of a ColE1 plasmid in Escherichia coli and antagonises RNaseHI RNA Biology. 10(5), 35-43
• IVANČIĆ-BAĆE I, AL HOWARD J and BOLT EL, 2012. Tuning in to interference: R-loops and Cascade complexes in CRISPR immunity.: Pubmed 22743103 Journal of Molecular Biology.
• HOWARD, J.A.L., DELMAS, S., IVANČIĆ‑BAĆE, I. and BOLT, E.L., 2011. Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein Biochemical Journal. 439(1), 85-95
• WOODMAN, I.L., BRAMMER, K. and BOLT, E.L., 2011. Physical interaction between archaeal DNA repair helicase Hel308 and Replication Protein A (RPA) DNA Repair. 10(3), 306-313
• MATILLA, I., ALFONSO, C., RIVAS, G., BOLT, E.L., DE LA CRUZ, F. and CABEZON, E., 2010. The conjugative DNA translocase TrwB is a structure-specific DNA-binding protein The Journal of Biological Chemistry. 285(23), 17537-17544
• MACHWE,, XIAO,, LLOYD,, BOLT, and ORREN,, 2007. Replication fork regression in vitro by the Werner syndrome protein (WRN): Holliday junction formation, the effect of leading arm structure and a potential role for WRN exonuclease activity. Nucleic Acids Research. 35(17), 5729-47
• WOODMAN, ISABEL L, BRIGGS, GEOFFREY S and BOLT, EDWARD L, 2007. Archaeal Hel308 domain V couples DNA binding to ATP hydrolysis and positions DNA for unwinding over the helicase ratchet. Journal Molecular Biology. 374(5), 1139-44
• MACMASTER, R, SEDELNIKOVA, S, BAKER, P.J, BOLT, E.L, LLOYD, R.G and RAFFERTY, J.B., 2006. RusA Holliday junction resolvase: DNA complex structure—insights into selectivity and specificity Nucleic Acids Research. 34(19), 5577–5584
• GUY, C.P., HALDENBY, S., BRINDLEY, A., WALSH, D.A., BRIGGS, G.S., WARREN, M.J., ALLERS, T. and BOLT, E.L., 2006. Interactions of RadB, a DNA repair protein in archaea, with DNA and ATP Journal of Molecular Biology. 358(1), 46-56
• GUY, C.P. and BOLT, E.L., 2005. Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands Nucleic Acids Research. 33(11), 3678-3690
• BOLT, E. L., 2005. Helicases that interact with replication forks: new candidates from archaea Biochemical Society Transactions. VOL 33(PART 6), 1471-1473
• GUY, C.P., MAJERNIK, A.I., CHONG, J.P.J. and BOLT, E.L., 2004. A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system Nucleic Acids Research. 32(21), 6176-6186
• SHARPLES, G. J., CURTIS, F. A., MCGLYNN, P. and BOLT, E. L., 2004. Holliday Junction Binding and Resolution by the Rap Structure-specific Endonuclease of Phage l Journal of Molecular Biology. VOL 340(NUMBER 4), 739-751
• RAFFERTY, J.B., BOLT, E.L., MURANOVA, T.A., SEDELNIKOVA, S.E., LEONARD, P., PASQUO, A., BAKER, P.J., RICE, D.W., SHARPLES, G.J. and LLOYD, R.G., 2003. The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold Structure. 11(12), 1557-1567
• MURANOVA, T A., SEDELNIKOVA, S.E., LEONARD, P.M., PASQUO, A., BOLT, E.L., LLOYD, R.G. and RAFFERTY, J.B., 2003. Crystallization of RusA Holliday junction resolvase from Escherichia coli Acta Crystallographica. Section D: Biological Crystallography. 59(12), 2262-2264
• BOLT, E. L. and GUY, C. P., 2003. Homologous recombination in Archaea: new Holliday junction helicases Biochemical Society Transactions. VOL 31(PART 3), 703-705
• BOLT, E.L. and LLOYD, R.G., 2002. Substrate specificity of RusA resolvase reveals the DNA structures targeted by RuvAB and RecG in vivo Molecular Cell. 10(1), 187-198
• SHARPLES, G.J., BOLT, E.L. and LLOYD, R.G., 2002. RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions Molecular Microbiology. 44(2), 549-560
• BOLT, E.L., LLOYD, R.G. and SHARPLES, G.J., 2001. Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli Journal of Molecular Biology. 310(3), 577-589