Ed Bolt

Contact

• workRoom D53 The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30194
• fax0115 82 30142
• ed.bolt@nottingham.ac.uk
• http://www.nottingham.ac.uk/life-sciences/people/ed.bolt

Biography

University of London: BSc. & PhD 1989-1995

Wellcome Trust Research Career development Fellowship, University of Nottingham 2002-2006

University Lecturer/Assistant Professor, University of Nottingham 2006-present

Expertise Summary

Cancer Biology

CRISPR biology (prokaryotes)

CRISPR biotechnology

Teaching Summary

I convene modules about molecular biology of DNA repair, CRISPR systems and RNA biology more generally.

Undergraduate projects join my group for funded summer vacation studentships and final year projects investigating the biology of CRISPR systems in bacteria, and mechanisms of DNA repair.

Research Summary

My group is currently (2022) funded by BBSRC responsive-mode project grants and by drug-discovery companies to investigate cancer biology and CRISPR.

Further information can be seen in Orcid; https://orcid.org/0000-0002-5656-7706

Selected Publications

• IVANA IVANCIC-BACE, SIMON D CASS, STEPHEN J WEARNE and EDWARD L BOLT, 2015. Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research.
• ROLLIE, CLARE, SCHNEIDER, STEFANIE, BRINKMANN, ANNA SOPHIE, BOLT, EDWARD L and WHITE, MALCOLM F, 2015. Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife. 4,
• CASS SD, HAAS KA, STOLL B, ALKHNBASHI O, SHARMA K, URLAUB H, BACKOFEN R, MARCHFELDER A and BOLT EL, 2015. The role of Cas8 in type I CRISPR interference BioScience Reports. 35(3), e00197
• GONG B, SHIN M, SUN J, JUNG CH, BOLT EL, VAN DER OOST J and KIM JS, 2014. Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proc Natl Acad Sci U S A.

• View all publications

• BBSRC Pool of Experts, 2022-2025
• Scientific advisory board of Amphista Therapeutics, 2020-ongoing
• Consultant Scientist for Oxford Nanopore Technologies, 2010-2017
• Member of the Microbiology Society Impact and Influence Committee, 2019-ongoing

· Editorial board and associate editor for Portland Press, Royal Society and Frontiers Journals

· Guest editor, Genes (CRISPR) and Frontiers in Molecular Biosciences (Recombination-Repair)

· Grant application reviewer for BBSRC, MRC, Wellcome Trust, NSF (USA), ANR (France), DFF (Denmark) and ITC/ITSP (Hong Kong).

· PhD degree examiner, recently at Universities of Oxford (2021), St. Andrews (2021), Huddersfield (2020), and Sheffield (2019).

· Reviewer for, recently, Nature Communications, Nucleic Acids Research, ACS Synthetic Biology, Genes, Journal of Molecular Biology.

Past Research

Please search PubMed for 'Edward_Bolt'

• BUCKLEY R, KRAMM K, COOPER CDO, GROHMANN D and BOLT EL, 2020. Mechanistic insights into Lhr helicase function in DNA repair. The Biochemical journal. 477(16), 2935-2947
• SEYS FM, ROWE P, BOLT EL, HUMPHREYS CM and MINTON NP, 2020. A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences. Genes. 11(4),
• HE L, ST JOHN JAMES M, RADOVCIC M, IVANCIC-BACE I and BOLT EL, 2020. Cas3 Protein-A Review of a Multi-Tasking Machine. Genes. 11(2),
• CHUN HANG LAU and EDWARD BOLT, 2020. Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9 Bioscience Reports.
• LAU CH, REEVES R and BOLT EL, 2019. Adaptation processes that build CRISPR immunity: creative destruction, updated. Essays in biochemistry. 63(2), 227-235
• KILLELEA T, HAWKINS M, HOWARD JL, MCGLYNN P and BOLT EL, 2018. DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase. RNA biology. PMID: 30096986
• RADOVCIC M, KILLELEA T, SAVITSKAYA E, WETTSTEIN L, BOLT EL and IVANCIC-BACE I, 2018. CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases. Nucleic acids research. PMID: 30189098
• CUBBON A, IVANCIC-BACE I and BOLT EL, 2018. CRISPR-Cas immunity, DNA repair and genome stability. Bioscience reports. 38(5), PMID: 30209206
• NORTHALL, S., BUCKLEY, R., JONES, N., PENEDO, C., SOULTANAS, P. and BOLT, E., 2017. DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain. DNA Repair (Amst). 57, 125-132
• MAJSEC K, BOLT EL and IVANČIĆ-BAĆE I, 2016. Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS. BMC microbiology. 16, 28
• NORTHALL SJ, IVANČIĆ-BAĆE I, SOULTANAS P and BOLT EL, 2016. Remodeling and Control of Homologous Recombination by DNA Helicases and Translocases that Target Recombinases and Synapsis. Genes. 7(8),
• BOLT, EDWARD L., JENKINS, TABITHA, RUSSO, VALERIA MOREIRA, AHMED, SHARLENE, CAVEY, JAMES and CASS, SIMON D., 2016. Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair BIOSCIENCE REPORTS. 36,
• CASS SD, HAAS KA, STOLL B, ALKHNBASHI O, SHARMA K, URLAUB H, BACKOFEN R, MARCHFELDER A and BOLT EL, 2015. The role of Cas8 in type I CRISPR interference BioScience Reports. 35(3), e00197
• ROLLIE, CLARE, SCHNEIDER, STEFANIE, BRINKMANN, ANNA SOPHIE, BOLT, EDWARD L and WHITE, MALCOLM F, 2015. Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife. 4,
• IVANA IVANCIC-BACE, SIMON D CASS, STEPHEN J WEARNE and EDWARD L BOLT, 2015. Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research.
• GONG B, SHIN M, SUN J, JUNG CH, BOLT EL, VAN DER OOST J and KIM JS, 2014. Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proceedings of the National Academy of Sciences of the United States of America. 111(46), 16359-64
• IVANČIĆ-BAĆE, I., RADOVČIĆ, M., BOČKOR, L., HOWARD, J.L. and BOLT, E.L., 2013. Cas3 stimulates runaway replication of a ColE1 plasmid in Escherichia coli and antagonises RNaseHI RNA Biology. 10(5), 35-43
• IVANČIĆ-BAĆE I, AL HOWARD J and BOLT EL, 2012. Tuning in to interference: R-loops and Cascade complexes in CRISPR immunity.: Pubmed 22743103 Journal of Molecular Biology.
• HOWARD, J.A.L., DELMAS, S., IVANČIĆ‑BAĆE, I. and BOLT, E.L., 2011. Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein Biochemical Journal. 439(1), 85-95
• WOODMAN, I.L., BRAMMER, K. and BOLT, E.L., 2011. Physical interaction between archaeal DNA repair helicase Hel308 and Replication Protein A (RPA) DNA Repair. 10(3), 306-313
• MATILLA, I., ALFONSO, C., RIVAS, G., BOLT, E.L., DE LA CRUZ, F. and CABEZON, E., 2010. The conjugative DNA translocase TrwB is a structure-specific DNA-binding protein The Journal of Biological Chemistry. 285(23), 17537-17544
• MACHWE,, XIAO,, LLOYD,, BOLT, and ORREN,, 2007. Replication fork regression in vitro by the Werner syndrome protein (WRN): Holliday junction formation, the effect of leading arm structure and a potential role for WRN exonuclease activity. Nucleic Acids Research. 35(17), 5729-47
• WOODMAN, ISABEL L, BRIGGS, GEOFFREY S and BOLT, EDWARD L, 2007. Archaeal Hel308 domain V couples DNA binding to ATP hydrolysis and positions DNA for unwinding over the helicase ratchet. Journal Molecular Biology. 374(5), 1139-44
• MACMASTER, R, SEDELNIKOVA, S, BAKER, P.J, BOLT, E.L, LLOYD, R.G and RAFFERTY, J.B., 2006. RusA Holliday junction resolvase: DNA complex structure—insights into selectivity and specificity Nucleic Acids Research. 34(19), 5577–5584
• GUY, C.P., HALDENBY, S., BRINDLEY, A., WALSH, D.A., BRIGGS, G.S., WARREN, M.J., ALLERS, T. and BOLT, E.L., 2006. Interactions of RadB, a DNA repair protein in archaea, with DNA and ATP Journal of Molecular Biology. 358(1), 46-56
• GUY, C.P. and BOLT, E.L., 2005. Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands Nucleic Acids Research. 33(11), 3678-3690
• BOLT, E. L., 2005. Helicases that interact with replication forks: new candidates from archaea Biochemical Society Transactions. VOL 33(PART 6), 1471-1473
• GUY, C.P., MAJERNIK, A.I., CHONG, J.P.J. and BOLT, E.L., 2004. A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system Nucleic Acids Research. 32(21), 6176-6186
• SHARPLES, G. J., CURTIS, F. A., MCGLYNN, P. and BOLT, E. L., 2004. Holliday Junction Binding and Resolution by the Rap Structure-specific Endonuclease of Phage l Journal of Molecular Biology. VOL 340(NUMBER 4), 739-751
• RAFFERTY, J.B., BOLT, E.L., MURANOVA, T.A., SEDELNIKOVA, S.E., LEONARD, P., PASQUO, A., BAKER, P.J., RICE, D.W., SHARPLES, G.J. and LLOYD, R.G., 2003. The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold Structure. 11(12), 1557-1567
• MURANOVA, T A., SEDELNIKOVA, S.E., LEONARD, P.M., PASQUO, A., BOLT, E.L., LLOYD, R.G. and RAFFERTY, J.B., 2003. Crystallization of RusA Holliday junction resolvase from Escherichia coli Acta Crystallographica. Section D: Biological Crystallography. 59(12), 2262-2264
• BOLT, E. L. and GUY, C. P., 2003. Homologous recombination in Archaea: new Holliday junction helicases Biochemical Society Transactions. VOL 31(PART 3), 703-705
• BOLT, E.L. and LLOYD, R.G., 2002. Substrate specificity of RusA resolvase reveals the DNA structures targeted by RuvAB and RecG in vivo Molecular Cell. 10(1), 187-198
• SHARPLES, G.J., BOLT, E.L. and LLOYD, R.G., 2002. RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions Molecular Microbiology. 44(2), 549-560
• BOLT, E.L., LLOYD, R.G. and SHARPLES, G.J., 2001. Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli Journal of Molecular Biology. 310(3), 577-589