Joëlle Goulding (née Alcock) graduated from the University of Nottingham with a BSc (Hons) in Genetics (2001) and went on to complete a PhD within the Institute of Genetics (2005). Subsequent postdoctoral work involved investigations into adult stem cells of the cerebellum. Joëlle then joined the Cell Signalling Research Group within the University of Nottingham (2007) changing research fields and prompting a strong focus on Molecular Pharmacology. Through studies of endogenous and transgenic expression systems of the adenosine and adrenergic receptors utilising protein-tags, biosensors and fluorescent ligands, Joëlle has built up a wide technological knowledge base. In 2009 Joëlle visited the laboratory of Prof. Carsten Hoffman, Wurzberg Germany, to learn a FlAsh-based FRET technique to study GPCR activation. In 2015 she was awarded a Diploma in Advanced Pharmacology by the British Pharmacological Society. More recently Joëlle has gained an Associate Fellowship of the Higher Education Academy.
In April 2017 Joëlle took up the position of Research Fellow in Advanced Microscopy within COMPARE at Nottingham University. She will be developing research within fluorescence correlation spectroscopy alongside testing methodologies for the study of membrane receptors with the newly acquired Olympus LV200 and PhaseFocus Livecyte.
Joëlle has a passion for imaging techniques and has implemented this passion by developing and optimising strategies to study receptor function.
· Light, confocal and wide-field microscopy (Zeiss LSM 510, 710, 880, Exciter, DeltaVision Elite)
· TIRF (total internal reflection fluorescence microscopy)
· FCS (Fluorescence correlation spectroscopy)
· High-throughput microscopy (Molecular Devices Ultra and Micro confocal plate readers)
· Perfusion - as used in http://dx.doi.org/10.1124/mol.110.064493
· Data analysis - MetaXpress, Volocity, GraphPad Prism
Additional laboratory-based expertise
· Cell Signalling assays/biosensors - including cAMP, Ca2+, ERK; techniques including FLEX, FRET and BRET
· Cell culture - Generation of primary cell lines, transient transfection and generation of stable cell lines
· Radioligand binding, competition and accumulation assays
Maths in the Lab - Essential maths skills in day-to-day lab work (Graduate school course)
A13GPD - GPCR polymorphisms, disease and personalised medicine (Year 3, BMedSci)
B33PPM - GPCR based personalised medicine (Year 3, MPharm)
Joëlle is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of… read more
STODDART, LEIGH A., JOHNSTONE, ELIZABETH K. M., WHEAL, AMANDA J., GOULDING, JOELLE, ROBERS, MATTHEW B., MACHLEIDT, THOMAS, WOOD, KEITH V., HILL, STEPHEN J. and PFLEGER, KEVIN D. G., 2015. Application of BRET to monitor ligand binding to GPCRs NATURE METHODS. 12(7), 661-663 ALCOCK, J., WARREN, A.Y., GOODSON, Y.J., HILL, S.J., KHAN, R.N. and LYMN, J.S., 2010. Inhibition of tissue transglutaminase 2 attenuates contractility of pregnant human myometrium Biology of Reproduction. 84(4), 646-653
ALCOCK, JOELLE, LOWE, JAMES, ENGLAND, TIM, BATH, PHILIP and SOTTILE, VIRGINIE, 2009. Expression of Sox1, Sox2 and Sox9 is maintained in adult human cerebellar cortex. Neuroscience Letters. 450(2), 114-6
Joëlle is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of endogenously expressed receptors.
Check out https://www.imagingscientist.com/ ( https://www.imagingscientist.com/joelle-goulding.html ) for a feel for the role of the Imaging Scientist in todays scientific research.