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Paddy Tighe

Associate Professor, Faculty of Medicine & Health Sciences

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Biography

My lab has interests in rare autoinflammatory conditions and systemic autoimmune diseases and I has spent many years investigating the molecular mechanisms associated with defects in the TNFR1 receptor which give rise to TNF-receptor-associated periodic syndrome (TRAPS). Over this time I have developed a number of methodologies for high-thoughput, high-content screening to enable wide-scale analysis of intrasignalling pathways within cells and serum-based assays (autoantibodies, pathogen-specific antibodies and other serum markers). This has lead to successful MRC applications in the area of drug repurposing in the context of the autoinflammatory diseases TRAPS, and subsequently to funding and collaborations applying these technologies for cancer early diagnosis (autoantibody detection) and alternatives to TMA tumour staining (tumour antigen detection), analysis and drug repurposing in systemic lupus erythematosus and ongoing projects in immune response surveillance in relation to influenza vaccine administration and a range of conditions including rheumatoid arthritis, Myasthenia gravis, H. pylori and C. difficile infections. I have current collaborations with both optical and electronics engineers in terms of label-free immunosensing methodologies.

Expertise Summary

I am an immunologist with many years experience of molecular cloning technologies, Molecular Immunology, T cell receptor analysis, DNA fragment analysis (AFLP, RAPD, SNP analysis, microsatellites, DNA sequencing), fluorescent methods and real-time quantification, microarray and macroarray printing capabilities; automated colony picking; liquid handling robotics; lab-on-a-chip technologies; microarray probe generation and quality control; automated stations for moderate/high-throughput protein microarray analysis etc.

Teaching Summary

I am interested in distance learning technologies and virtual learning environments and how to enhance the interaction of students with materials on VLEs. I am a keen supporter of mixed media… read more

Research Summary

molecular mechanisms of (Auto)inflammatory disease and early diagnostics

We have spent many years studying Tumour necrosis factor receptor I (TNFRSF1A) mutations and the dominantly inherited autoinflammatory syndrome, TRAPS (TNF receptor-associated periodic syndrome). Our research encompasses mechanistic alterations within cells carrying the mutant receptor, the receptor mutants as a protein conformational disorder and investigation of novel treatments for TRAPS. This research encompasses a wide range of technology platforms including flow cytometry, Expression microarrays, quantitative PCR and extensive expression cloning and protein tagging methodologies. The R92Q polymorphism of TNFR1 is over-represented in numerous conditions including atherosclerosis, Rheumatoid arthritis and Behcets Disease. We are examining its influence at the molecular level in these diseases, especially atherosclerosis.

Allied to this is investigations into diagnosis and stratification of patients based upon montioring peripheral blood inflammatory markers which has since extended into early screening methods for autoantibody detection, in both systemic autoimmune diseases and secondarily for cancer early detection.

Selected Publications

I am interested in distance learning technologies and virtual learning environments and how to enhance the interaction of students with materials on VLEs. I am a keen supporter of mixed media podcasting and am involved in University funded video/screen podcasting developments.

Past Research

Autoimmunity (Autoimmune uveitis, SLE) peptide immunotherapeutics, phage display of peptides

Future Research

We aim to further explore TRAPS as a prototypic autoinflammatory disease, especially perturbations of heat shock proteins and ubiquitiination status associated with the pro-inflammatory signalling cascades

Secondarily we are developing our interests in novel detection methods ( quantum dot based) and microfluidics approaches to comprehensive diagnositic testing methods

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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