BSc in Biological Sciences (Molecular Biology) at the University of Exeter, 1996
DPhil in Medical Molecular Microbiology at the University of Oxford, 2000
Postdoctoral Researcher at the MRC, National Institute for Medical Research, London, 2000-2003
Postdoctoral Researcher at Imperial College, London, 2003-2007 (PI of NIH grant at the affiliated TB group at AHVLA 2005-2007)
Career break raising family 2007-2013
Postdoctoral Researcher conducting independent research (0.5 FTE), Kingston University, 2013-2015
Lecturer, School of LIfe Sciences, Kingston University, 2015-2017
Assistant Professor, School of Life Sciences, The University of Nottingham, 2017.
Ruth Griffin is an Assistant Professor at the BBSRC/EPSRC Synthetic Biology Research Centre (SBRC) at the University of Nottingham. Ruth supports Professor Nigel Minton's area of Health Research in developing new therapies for Clostridium difficile and is drawing from her expertise in vaccine development to manufacture novel lipoprotein-based vaccines against this organism.
Her passion for vaccine research began during her MRC-funded DPhil at the University of Oxford. Under the supervision of Professor Richard Moxon, she evaluated inner core lipopolysaccharide of Haemophilus influenzae as a potential vaccine antigen and discovered the role of a key locus, lex2 involved in antigenic variation of a major epitope that conferred resistance to serum killing. She then undertook a post-doctoral position at the National Institute for Medical Research in Mill Hill, London, to work on improving the immunogenicity of the BCG vaccine by generating recombinant strains expressing Mycobacterium tuberculosis antigens. In addition, she characterised the molecular pathway for the biosynthesis of p-hydroxy benzoic acid derivatives of M. tuberculosis and researched lipoarabinomannan biosynthesis. Ruth continued this work at Imperial College working with Professor Douglas Young and established a collaboration with Professor Mary Jackson at the Institut Pasteur. With further collaborations built with Professor Patrick Brennan at Colorado State University, an NIH collaborative grant was awarded and as joint PI, Ruth continued her research on Mycobacteria species at the Animal Health Veterinary Laboratories Agency.
Following a career break for several years raising her family, Ruth returned to research part time and as PI at her local University, Kingston, she evaluated Factor H binding protein as a vaccine antigen against meningococcal serogroup B disease. After 2 years, she became a full time Lecturer and secured funding from the Brazilian Foundation, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to fund a PhD student. Now, keen to apply her knowledge to other bacteria for which vaccines are urgently needed, Ruth is developing a lipoprotein-based vaccine platform to initially target C. difficile.
I previously taught undergraduates including first year students Molecular Genetics and delivered practicals on laboratory skills. I led practicals for second year students in enzyme kinetics and… read more
I am currently developing a vaccine to target Clostridium difficile for which there is no approved prophylactic. This bacterium is responsible for approximate 30, 000 deaths from the half a million… read more
GRIFFIN R and MINTON NP, 2017. Exposing hidden putative lipoproteins in Clostridium difficile In: ClostPath 10th International Conference on the Molecular Biology and Pathogenesis of the Clostridia.
DA SILVA RAG, CHURCHWARD CP, KARLYSHEV AV, ELEFTHERIADOU O, SNABAITIS AK, LONGMAN MR, RYAN A and GRIFFIN R, 2017. The role of apolipoprotein N-acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target. British journal of pharmacology. 174(14), 2247-2260
THOLE J, GRIFFIN R and YOUNG DB, 2009. The Future of Tuberculosis Vaccinology. In: RAVIGLIONE MC, ed., Lung Biology in Health and Disease Tuberculosis Fourth Edition The Essentials. 237. Informa Healthcare USA Inc., New York.
I previously taught undergraduates including first year students Molecular Genetics and delivered practicals on laboratory skills. I led practicals for second year students in enzyme kinetics and taught third year students Prokaryotic Genetics and Bioinformatics. I supervised final year project students and ran a first year module, Genes, Cells and Tissues.
I also taught Masters students, running practical classes on molecular methods to diagnose acute promyelocytic leukemia and supervising their research projects.
I am currently developing a vaccine to target Clostridium difficile for which there is no approved prophylactic. This bacterium is responsible for approximate 30, 000 deaths from the half a million infected individuals each year in the US alone costing around $5 billion/year. Over the last year in England and Wales there have been up to 13, 000 cases of C. difficile infection (CDI). With the rise in antimicrobial-resistant strains and hyper-virulent isolates, CDI has become a global health threat.
I an using reverse vaccinology (in silico approaches including genome mining and applying predictive algorithms), to identify conserved antigens that are likely to be surface-exposed. The genes encoding the selected antigens will be cloned and over-expressed in Escherichia coli then purified and their vaccine potential tested in vivo. My PhD student, Cansu Karyal, funded by the School of Life Sciences, will be conducting this research.
I am also continuing with the research I conducted at Kingston University investigating the molecular pathway for the lipidation and export of the meningococcal vaccine antigen, Factor H binding protein (FHbp) In particular my focus has been on identifying the reason why some strains are not capable of exporting this antigen to the cell surface thus escape immune recognition and are therefore non-vaccine preventable by FHbp-based vaccines. This knowledge is important for generating improved vaccines. My PhD student, Ronni da Silva who is funded from the Brazilian Science without Borders program, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) has been working on this project for the past 2 years at Kingston University and will be transferring to The University of Nottingham to continue his PhD with me.
My interest in developing vaccines against pathogenic bacteria began during my DPhil at the University of Oxford. Here I investigated the conservancy of lipopolysaccharide of Haemophilus influenzae, a candidate being tested at the time. With the breakthrough of the Hib vaccine successfully combating childhood meningitis caused by this bacterium, I went on to research a more life threatening bacterium, Mycobacterium tuberculosis for which improved vaccines were urgently needed. At the National Institute for Medical Research I investigated lipoarabinonmannan as a possible vaccine antigen. In addition, I generated recombinant Mycobacterium bovis BCG over-expressing potent M. tuberculosis antigens to improve its immunogenicity. After securing funding from the NIH from a collaboration with Colorado State University and Institut Pasteur, I worked as PI at Imperial College then at the affiliated TB group at the AHVLA.
Following a career break raising my family, I chose to research Neisseria meningitidis, another bacterium that causes meningitis and septicaemia, at my local University, Kingston London. .
The research I have conducted on each bacterium has involved identifying the genes involved in the biosynthesis, assembly and export of vaccine antigens. A fundamental understanding of the molecular mechanism of expression of surface antigens moreover inefficiencies or failure to do so by certain strains enables improved prediction of vaccine efficacy as well as more robust evaluation of clinical trial data.
Upon success of developing an effective vaccine against C. difficile, I would like to expand my vaccine research to target other bacterial pathogens including Neisseria gonorrhoeae.