Steve Briddon

Contact

• workRoom CS5 The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30089
• fax0115 82 30081
• stephen.briddon@nottingham.ac.uk

Biography

Steve is both a Principal Research Fellow at the University of Nottingham and since Sep 2024, Director of Pharmacology at Excellerate Bioscience. He has nearly 30 years' experience as a molecular pharmacologist, with a focus on the G protein-coupled receptors. His particular interest is in the spatio-temporal organisation of receptors at the subcellular level, and has spent much of the last 20 years developing advanced imaging and spectroscopy approaches to studying GPCR pharmacology in single cells. He is a Fellow of the British Pharmacological Society and the Royal Microscopical Society, as well as member of the Editorial board for Pharmacology Matters

Expertise Summary

• Molecular Pharmacology of GPCRs (Quantitative Pharmacology, NanoBRET, TR-FRET)
• Fluorescence Correlation Spectroscopy (FCS, FCCS, RICS, PCH)
• Single cell pharmacology (binding and function)
• Live cell confocal microscopy (fluorescent ligand binding, receptor trafficking)
• Advanced imaging approaches (FLIM, FRAP)
• Single cell calcium measurements
• Second messenger assays (inositol phosphates, cAMP)

Selected Publications

• KILPATRICK, LE, FRIEDMAN-OHANA, R, ALCOBIA, DC, RICHING, K, PEACH, CJ, WHEAL, AJ, BRIDDON, SJ, ROBERS, MB, ZIMMERMAN, K, MACHLEIDT, T, WOOD, KV, WOOLARD, J and HILL, SJ, 2017. Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes. Biochemical Pharmacology. 136, 62-75
• WONG K, BRIDDON SJ, HOLLIDAY ND and KERR ID, 2015. Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques. Biochimica et biophysica acta. 1863(1), 19-29
• GHERBI K, MAY LT, BAKER JG, BRIDDON SJ and HILL SJ, 2015. Negative cooperativity across β1-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(7), 2859-71
• CORRIDEN R, KILPATRICK LE, KELLAM B, BRIDDON SJ and HILL SJ, 2014. Kinetic analysis of antagonist-occupied adenosine-A3 receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism. FASEB journal. 28, 4211-4222

• View all publications

• GONDIN, ARISBEL B., HALLS, MICHELLE L., CANALS, MERITXELL and BRIDDON, STEPHEN J., 2019. GRK Mediates mu-Opioid Receptor Plasma Membrane Reorganization FRONTIERS IN MOLECULAR NEUROSCIENCE. 12,
• ADLERE, I., SUN, S., ZARCA, A., ROUMEN, L., GOZELLE, M., VICIANO, C. PERPINA, CASPAR, B., ARIMONT, M., BEBELMAN, J. P., BRIDDON, S. J., HOFFMANN, C., HILL, S. J., SMIT, M. J., VISCHER, H. F., WIJTMANS, M., DE GRAAF, C., DE ESCH, I. J. P. and LEURS, R., 2019. Structure-based exploration and pharmacological evaluation of N-substituted piperidin-4-yl-methanamine CXCR4 chemokine receptor antagonists EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. 162, 631-649
• BOUZO-LORENZO, MONICA, STODDART, LEIGH A., XIA, LIZI, IJZERMAN, ADRIAAN P., HEITMAN, LAURA H., BRIDDON, STEPHEN J. and HILL, STEPHEN J., 2019. A live cell NanoBRET binding assay allows the study of ligand-binding kinetics to the adenosine A(3) receptor PURINERGIC SIGNALLING. 15(2), 139-153
• BOSMA, REGGIE, STODDART, LEIGH A., GEORGI, VICTORIA, BOUZO-LORENZO, MONICA, BUSHBY, NICK, INKOOM, LORETTA, WARING, MICHAEL J., BRIDDON, STEPHEN J., VISCHER, HENRY F., SHEPPARD, ROBERT J., FERNANDEZ-MONTALVAN, AMAURY, HILL, STEPHEN J. and LEURS, ROB, 2019. Probe dependency in the determination of ligand binding kinetics at a prototypical G protein-coupled receptor SCIENTIFIC REPORTS. 9, 7906
• ARRUDA MA, STODDART LA, GHERBI K, BRIDDON SJ, KELLAM B and HILL SJ, 2018. A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A3 Receptor in Living Cells. Frontiers in pharmacology. 8, 908
• BRIDDON, STEPHEN J., KILPATRICK, LAURA E. and HILL, STEPHEN J., 2018. Studying GPCR Pharmacology in Membrane Microdomains: Fluorescence Correlation Spectroscopy Comes of Age TRENDS IN PHARMACOLOGICAL SCIENCES. 39(2), 158-174
• STODDART, LEIGH A., VERNALL, ANDREA J., BOUZO-LORENZO, MONICA, BOSMA, REGGIE, KOOISTRA, ALBERT J., DE GRAAF, CHRIS, VISCHER, HENRY F., LEURS, ROB, BRIDDON, STEPHEN J., KELLAM, BARRIE and HILL, STEPHEN J., 2018. Development of novel fluorescent histamine H-1-receptor antagonists to study ligand-binding kinetics in living cells SCIENTIFIC REPORTS. 8, 1572
• GHERBI, KAROLINA, BRIDDON, STEPHEN J. and CHARLTON, STEVEN J., 2018. Micro-pharmacokinetics: Quantifying local drug concentration at live cell membranes SCIENTIFIC REPORTS. 8,
• LAM, RAYMOND, GONDIN, ARISBEL B., CANALS, MERITXELL, KELLAM, BARRIE, BRIDDON, STEPHEN J., GRAHAM, BIM and SCAMMELLS, PETER J., 2018. Fluorescently Labeled Morphine Derivatives for Bioimaging Studies JOURNAL OF MEDICINAL CHEMISTRY. 61(3), 1316-1329
• KILPATRICK, LE, FRIEDMAN-OHANA, R, ALCOBIA, DC, RICHING, K, PEACH, CJ, WHEAL, AJ, BRIDDON, SJ, ROBERS, MB, ZIMMERMAN, K, MACHLEIDT, T, WOOD, KV, WOOLARD, J and HILL, SJ, 2017. Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes. Biochemical Pharmacology. 136, 62-75
• LOCHRAY, J., GHERBI, K., SYKES, D. A., BRIDDON, S. and CHARLTON, S. J., 2017. MEASURING LIGAND CONCENTRATION WHERE IT MATTERS: ASSESSING THE "MICRO PK/PD" OF ADENOSINE A2A RECEPTOR LIGANDS BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY. 121, 17-17
• SCHEMBRI LS, STODDART LA, BRIDDON SJ, KELLAM B, CANALS M, GRAHAM B and SCAMMELLS PJ, 2015. Synthesis, Biological Evaluation, and Utility of Fluorescent Ligands Targeting the μ-Opioid Receptor. Journal of medicinal chemistry. 58(24), 9754-67
• STODDART LA, VERNALL AJ, BRIDDON SJ, KELLAM B and HILL SJ, 2015. Direct visualisation of internalization of the adenosine A3 receptor and localization with arrestin3 using a fluorescent agonist. Neuropharmacology. 98, 68-77
• STODDART LA, KILPATRICK LE, BRIDDON SJ and HILL SJ, 2015. Probing the pharmacology of G protein-coupled receptors with fluorescent ligands. Neuropharmacology. 98, 48-57
• GHERBI K, MAY LT, BAKER JG, BRIDDON SJ and HILL SJ, 2015. Negative cooperativity across β1-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(7), 2859-71
• WONG K, BRIDDON SJ, HOLLIDAY ND and KERR ID, 2015. Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques. Biochimica et biophysica acta. 1863(1), 19-29
• GHERBI K, BRIDDON SJ and HILL SJ, 2014. Detection of the secondary low affinity β1 -adrenoceptor site in living cells using the fluorescent CGP 12177 derivative BODIPY-TMR-CGP. British journal of pharmacology. 171, 5431-5445
• CORRIDEN R, KILPATRICK LE, KELLAM B, BRIDDON SJ and HILL SJ, 2014. Kinetic analysis of antagonist-occupied adenosine-A3 receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism. FASEB journal. 28, 4211-4222
• STODDART LA, KELLAM B, BRIDDON SJ and HILL SJ, 2014. Effect of a toggle switch mutation in TM6 of the human adenosine A₃ receptor on Gi protein-dependent signalling and Gi-independent receptor internalization. British journal of pharmacology. 171(16), 3827-44
• ARRUDA, MARIA AUGUSTA, STODDART, LEIGH A., BRIDDON, STEPHEN J., KELLAM, BARRIE and HILL, STEPHEN J., 2014. Monitoring ligand-binding to A(3)AR in living cells using fluorescent ligands: Application to high throughput screening of chemical libraries PURINERGIC SIGNALLING. 10(4), 754-754
• CORRIDEN, R., SELF, T., AKONG-MOORE, K., NIZET, V., KELLAM, B., BRIDDON, S.J. and HILL, S.J., 2013. Adenosine-A₃ receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes EMBO Reports. 14(8), 726-732
• VERNALL AJ, STODDART LA, BRIDDON SJ, NG HW, LAUGHTON CA, DOUGHTY SW, HILL SJ and KELLAM B, 2013. Conversion of a non-selective adenosine receptor antagonist into A3-selective high affinity fluorescent probes using peptide-based linkers. Organic & biomolecular chemistry. 11(34), 5673-82
• STODDART LA, BRIDDON SJ and HILL SJ, 2013. Fluorescent ligands for G protein-coupled receptors: illuminating receptor-ligand interactions for drug discovery. Future medicinal chemistry. 5(12), 1367-9
• ROSE, R.H., BRIDDON, S.J. and HILL, S.J., 2012. A novel fluorescent histamine H₁ receptor antagonist demonstrates the advantage of using fluorescence correlation spectroscopy to study the binding of lipophilic ligands British Journal of Pharmacology. 165(6), 1789-1800
• VERNALL, A.J., STODDART, L.A., BRIDDON, S.J., HILL, S.J. and KELLAM, B., 2012. Highly potent and selective fluorescent antagonists of the human adenosine A₃ receptor based on the 1,2,4-triazolo[4,3-α]quinoxalin-1-one scaffold Journal of Medicinal Chemistry. 55(4), 1771-1782
• STODDART, L.A., VERNALL, A.J., DENMAN, J.L., BRIDDON, S.J., KELLAM, B. and HILL, S.J., 2012. Fragment screening at adenosine-A₃ receptors in living cells using a fluorescence-based binding assay Chemistry & Biology. 19(9), 1105-1115
• AYLING LJ, BRIDDON SJ, HALLS ML, HAMMOND GR, VACA L, PACHECO J, HILL SJ and COOPER DM, 2012. Adenylyl cyclase AC8 directly controls its micro-environment by recruiting the actin cytoskeleton in a cholesterol-rich milieu. Journal of cell science. 125(Pt 4), 869-86
• BRIDDON, STEPHEN J, KELLAM, BARRIE and HILL, STEPHEN J, 2011. Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells. Methods in Molecular Biology. 746, 211-36
• MAY, L.T., BRIDGE, L.J., STODDART, L.A., BRIDDON, S.J. and HILL, S.J., 2011. Allosteric interactions across native adenosine-A₃ receptor homodimers: quantification using single-cell ligand-binding kinetics FASEB Journal. 25(10), 3465-3476
• BAKER, J.G., MIDDLETON, R., ADAMS, L., MAY, L.T., BRIDDON, S.J., KELLAM, B. and HILL, S.J., 2010. Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A₁ receptor ligands British Journal of Pharmacology. 159(4), 772-786
• KILPATRICK, L.E., BRIDDON, S.J., HILL, S.J. and HOLLIDAY, N.D., 2010. Quantitative analysis of neuropeptide Y receptor association with β-arrestin2 measured by bimolecular fluorescence complementation British Journal of Pharmacology. 160(4), 892-906
• MAY, L T, BRIDDON, S J and HILL, S J, 2010. Antagonist selective modulation of adenosine A1 and A3 receptor pharmacology by the food dye Brilliant Black BN: evidence for allosteric interactions. Molecular Pharmacology. 77(4), 678-86
• MAY, L.T., SELF, T.J., BRIDDON, S.J. and HILL, S.J., 2010. The effect of allosteric modulators on the kinetics of agonist-G protein-coupled receptor interactions in single living cells Molecular Pharmacology. 78(3), 511-523
• ROSE, RH, BRIDDON, SJ and HOLLIDAY, ND, 2010. Bimolecular Fluorescence Complementation:Lighting Up Seven Transmembrane Domain Receptor Signalling Networks British Journal of Pharmacology. 738-750
• HOBBS, H., BRIDDON, S.J. and LESTER, E., 2009. The synthesis and fluorescent properties of nanoparticulate ZrO₂doped with Eu using continuous hydrothermal synthesis Green Chemistry. 11(4), 484-491
• COUPLAND, P.G., BRIDDON, S.J. and AYLOTT, J.W., 2009. Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery Integrative Biology. 1(4), 318-323
• CORDEAUX, Y., BRIDDON, S.J., ALEXANDER, S.P.H., KELLAM, B. and HILL, S.J., 2008. Agonist-occupied A₃ adenosine receptors exist within heterogeneous complexes in membrane microdomains of individual living cells FASEB Journal. 22(3), 850-860
• BRIDDON, S.J., GANDÍA, J., AMARAL, O.B., FERRÉ, S., LLUÍS, C., FRANCO, R., HILL, S.J. and CIRUELA, F., 2008. Plasma membrane diffusion of g protein-coupled receptor oligomers Biochimica et Biophysica Acta: Molecular Cell Research. 1783(12), 2262-2268
• MIDDLETON,R, BRIDDON,S.J., CORDEAUX,Y., YATES,A.S., DALE,C.L., GEORGE,M., BAKER,J.G., HILL,S.J. and KELLAM,B., 2007. New Fluorescent Adenosine A(1)-Receptor Agonists That Allow Quantification of Ligand-Receptor Interactions in Microdomains of Single Living Cells. Journal of Medicinal Chemistry. 50(4), 782-793
• BRIDDON, and HILL,, 2007. Pharmacology under the microscope: the use of fluorescence correlation spectroscopy to determine the properties of ligand-receptor complexes. Trends in Pharmacological Sciences. 28, 637-645
• BRIDDON, S.J., MIDDLETON, R.J., CORDEAUX, Y., FLAVIN, F.M., WEINSTEIN, J.A., GEORGE, M.W., KELLAM, B. and HILL, S.J., 2004. Quantitative analysis of the formation and diffusion of A1-adenosine receptor-antagonist complexes in single living cells Proceedings of the National Academy of Sciences of the United States of America. 101(13), 4673-4678
• BRIDDON, S.J., MIDDLETON, R.J., YATES, A.S., GEORGE, M.W., KELLAM, B. and HILL, S.J., 2004. Application of fluorescence correlation spectroscopy to the measurement of agonist binding to a G-protein coupled receptor at the single cell level Faraday Discussions. 126, 197-207
• WEISSHART, K., JUNGEL, V. and BRIDDON, S. J., 2004. The LSM 510 META - ConfoCor 2 System: An Integrated Imaging and Spectroscopic Platform for Single-Molecule Detection Current Pharmaceutical Biotechnology. 5(2), 135-154
• BAUER, M, MASCHBERGER, P, QUEK, L, BRIDDON, SJ, DASH, D, WEISS, M, WATSON, SP and SIESS, W, 2001. Genetic And Pharmacological Analyses Of Involvement Of Src-Family, Syk And Btk Tyrosine Kinases In Platelet Shape Change - Src-Kinases Mediate Integrin Alpha(Iib)Beta(3) Inside-Out Signaling During Shape Change Thrombosis And Haemostasis. 85(2), 331-340
• CORDEAUX, Y, BRIDDON, S J, MEGSON, A E, MCDONNELL, J, DICKENSON, J M and HILL, S J, 2000. Influence of receptor number on functional responses elicited by agonists acting at the human adenosine A(1) receptor: evidence for signaling pathway-dependent changes in agonist potency and relative intrinsic activity. Molecular Pharmacology. 58(5), 1075-84
• BRIDDON, S J and WATSON, S P, 1999. Evidence for the involvement of p59fyn and p53/56lyn in collagen receptor signalling in human platelets. Biochemical Journal. 338 ( Pt 1), 203-9
• BRIDDON, S J, MELFORD, S K, TURNER, M, TYBULEWICZ, V and WATSON, S P, 1999. Collagen mediates changes in intracellular calcium in primary mouse megakaryocytes through syk-dependent and -independent pathways. Blood. 93(11), 3847-55
• GIBBINS, J. M., BRIDDON, S., SHUTES, A., VAN VUGT, M. J., VAN DE WINKEL, J. G. J., SAITO, T. and WATSON, S. P., 1998. The p85 subunit of phosphatidylinositol 3-kinase associates with the Fc receptor ç-chain and linker for activator of T cell (LAT) in platelets stimulated by collagen and convulxin Journal of Biological Chemistry. VOL 273(NUMBER 51), 34437-34443
• BRIDDON, S J, LESLIE, R A and ELLIOTT, J M, 1998. Comparative desensitization of the human 5-HT2A and 5-HT2C receptors expressed in the human neuroblastoma cell line SH-SY5Y. British Journal of Pharmacology. 125(4), 727-34
• MELFORD, S K, TURNER, M, BRIDDON, S J, TYBULEWICZ, V L and WATSON, S P, 1997. Syk and Fyn are required by mouse megakaryocytes for the rise in intracellular calcium induced by a collagen-related peptide. Journal of Biological Chemistry. 272(44), 27539-42
• ELLIOTT, J M, NEWBERRY, N R, CHOLEWINSKI, A J, BARTRUP, J T, BRIDDON, S J, CAREY, J E, FLANIGAN, T P, NEWTON, R A, PHIPPS, S L and REAVLEY, A C, 1995. Characterization of the 5-hydroxytryptamine2A receptor-activated cascade in rat C6 glioma cells. Neuroscience. 69(4), 1119-31
• BRIDDON, S, BECK, S A and TISDALE, M J, 1991. Changes in activity of lipoprotein lipase, plasma free fatty acids and triglycerides with weight loss in a cachexia model. Cancer Letters. 57(1), 49-53
• AYLING, LAURA J., BRIDDON, STEPHEN J., HALLS, MICHELLE L., HAMMOND, GERALD R. V., VACA, LUIS, PACHECO, JONATHAN, HILL, STEPHEN J. and COOPER, DERMOT M. F., Adenylyl cyclase AC8 directly controls its micro-environment by recruiting the actin cytoskeleton in a cholesterol-rich milieu JOURNAL OF CELL SCIENCE. 125(4), 869-886