I have spent my professional life applying microscopy and preparation techniques to answer complex scientific and biological research problems. I have roles in academia and for the Medical Research Council where I have supported full time research projects alongside setting up and developing high end imaging facilities at each institution. I have act as advisor for Zeiss, Molecular Devices, Phasefocus, Nikon, BBSRC on a number of occasions.
Founder member of UK Light Microscope Facility Managers Group and UkBioImaging. I am also Chair of the University of Nottingham Facility Manager Group and organised the inaugural Zeiss Elyra Super Resolution user group meeting in 2017.
My expertise gained from 35 years working with microscopes in biomedical research spans a wide range of imaging and preparation techniques. I can determine and apply on the most appropriate preparation technique for samples to visualise and study at the highest resolution such as Electron Microscopy to more simple light microscopy. I have a particular skills in live cell and fluorescence imaging gained from the last 17 years building and developing the facilities and applications at University of nottingham. I have a long track record of peer reviewed publications that cover a wide range of scientific research areas.
My expertise has been instrumental in the development and implementation of a number of successful multi million pound proramme grants awarded by MRC, BBSRC, Wellcome and SRIF. I am instrumental in the application and development of novel technology to answer important scientific questions through microscopy.
I am an Associate Fellow of the HEA and with my team run regular workshops for staff, Doctoral Training Programmes, post graduate students, undergraduate student practical classes. I also lecture to… read more
Applying microscopy and imaging to answer complex research questions.
Developing light microscope techniques with School of Life Sciences Imaging (SLIM) to bring understanding of microscopy and research to schools and the public through outreach activities and programmes.
SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24 KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
ORIHUELA, C.J., MAHDAVI, J., THORNTON, J., MANN, B., WOOLDRIDGE, K.G., ABOUSEADA, N., OLDFIELD, N.J., SELF, T., ALA'ALDEEN, D.A.A. and TUOMANEN, E.I., 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119(6), 1638-1646
I am an Associate Fellow of the HEA and with my team run regular workshops for staff, Doctoral Training Programmes, post graduate students, undergraduate student practical classes. I also lecture to MSC Stem cell students on fundamentals of light and fluorescence microscopy. Post graduate teaching is delivered through a number of two day workshops and can be summarised:
The aims of the course are to introduce the students to fundamentals of microscopy, preparation and image analysis. The course will involve a mix of theory talks and hands-on workshops using state of the art microscopes. By the end of the course the students will understand the principles of fluorescence, resolution, operation of the microscope and be able to analyse images acquired on some of the systems available in Life Sciences. The two-day workshop with consist of a series of lectures followed by hands on practical sessions.