Contact

• workRoom B207b Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 7485211
• Kenton.Arkill@nottingham.ac.uk

Research Summary

Being able to image the microcirculation is a key technology in the determination of vascular physiology. My research focuses on developing 3D light and electron microscopy techniques in order to… read more

Recent Publications

• DALWADI, MOHIT P., KING, JOHN R., DYSON, ROSEMARY J. and ARKILL, KENTON P., 2020. Mathematical model to determine the effect of a sub-glycocalyx space PHYSICAL REVIEW FLUIDS. 5(4),
• ALLEN, CLAIRE L, MALHI, NASEEB K, WHATMORE, JAQUELINE D, BATES, DAVID O and ARKILL, KENTON P, 2020. Non-invasive measurement of retinal permeability in a diabetic rat model. Microcirculation (New York, N.Y. : 1994).
• OWEN-WOODS, CHARLOTTE, JOULIA, REGIS, BARKAWAY, ANNA, ROLAS, LOIC, MA, BIN, NOTTEBAUM, ASTRID FEE, ARKILL, KENTON P, STEIN, MONJA, GIRBL, TAMARA, GOLDING, MATTHEW, BATES, DAVID O, VESTWEBER, DIETMAR, VOISIN, MATHIEU-BENOIT and NOURSHARGH, SUSSAN, 2020. Local microvascular leakage promotes trafficking of activated neutrophils to remote organs. The Journal of clinical investigation. 130(5), 2301-2318
• ASHWORTH, J. C., THOMPSON, J. L., JAMES, J. R., SLATER, C. E., PIJUAN-GALITO, S., LIS-SLIMAK, K., HOLLEY, R. J., MEADE, K. A., THOMPSON, A., ARKILL, K. P., TASSIERI, M., WRIGHT, A. J., FARNIE, G. and MERRY, C. L. R., 2020. Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro MATRIX BIOLOGY. 85-86, 15-33

• View all publications

Fellow of the Royal Microscopy Society

Member of the Institute of Physics

Member of the British Microcirculation Society (Committee 2015-2018)

Current Research

Being able to image the microcirculation is a key technology in the determination of vascular physiology. My research focuses on developing 3D light and electron microscopy techniques in order to better describe the microcirculation.

Funded Research

2018-2021 Newton Mobility Grant (The Royal Society), FRGS (Malaysian Research Council)

Understanding the Effect of Radiation on Biolubrication which Contributes to Joint Pain Post Cancer Radiation Treatment

This project is particularly interested in the proteoglycan lubricin and how its lipid and hyaluronan interactions change due to radiation therapy. Damage may be the route cause of arthritic pain. This as a new project area in collaboration with Dr Irman Rahman, and Dr Megan Lord.

2017-2020 Medical Research Council - New Investigator Research Grant

Variation in Glycocalyx Structure in Diabetes

Diabetic Retinopathy and Diabetic Nephropathy cause blindness and kidney failure associated by leaky blood vessels. The proposed research is focused on the endothelial glycocalyx. This is a specialised layer that covers blood vessel walls and acts as a molecular filter so the correct molecules transverse blood vessel walls. In normal physiology this filter is a layer of fibres spaced regularly at 20nm apart. The systemic disruption of this layer is well established in diabetes but what the disruption is, and what mechanism causes it, is totally unknown.

The overarching aim is to determine the basis of the filtration functionality by understanding the glycocalyx fibre organisational structure and how this is changed in diabetic model systems. The proposal will use state-of-the-art three dimensional, structural, and analytical electron microscopy techniques to determine structure whilst systematically altering the constituent components and functionality (permeability) of the glycocalyx.

PDRA: Dr Claire Allen

Main Collaborators: Prof. Dave Bates, Dr Cathy Merry, Dr Roland Fleck, Prof. Klaus Qvortrup.

Past Research

2016-2017 Facility for Environmental Nanoscience Characterisation and Analysis

Determining Dynamic Nanoparticle Uptake On Subcellular Scale Using Correlative light Electron Microscopy

Environmentally relevant nanoparticles (e.g. Silver used in industrial processes) have similar issues to medical nanoparticles used in drug delivery when it comes to detecting where in tissue they have gone. This project is to combine the knowledge of the medical field with the environmental field to create a combined workflow within the FENAC facility.

Main Collaborators: FENAC, Prof. Paul Verkade, Dr Lorna Hodgson and Mrs Judith Mantell

2015-2017 British Heart Foundation

Novel nanosensors for real time determination of shear stress experienced by the endothelial surface layer

The wall shear stress is the force of a fluid on a surface, and in blood vessels this force changes how the walls of the blood vessels behave. In diseases such as cancer, atherosclerosis and diabetes changes in the physical and biochemical behaviour are linked to the wall shear stress yet it cannot be measured accurately.

Myself, with collaborators from several universities, have designed a semi-rigid bio-synthetic nanoparticle that is shaped as a piece of string. It is 900nm long but only 7nm wide. We attach this to any surface using a surface dependent molecule and image how the particle reacts to fluids. The current work is to alter the design the particle to work in physiological environments.

Main Collaborators: Prof. Tim Dafforn, Prof. Dave Bates, Dr Dave Smith

2015-2017 Bizkaia Talent Fellowship - (University of the Basque Country)

Regulation of Nuclear Envelope Architecture: A Hallmark in Ageing

The internal membranes around the nucleus extend out into the whole cell. As tissues age the processes within cells dividing degrades. The membranes are made of a concoction of lipid bilayers that have different abilities to curve. This project aims to determine the curvature of lipids, hence their make-up and function, using a combination of 3D Correlative Light Electron Microscopy techniques.

Main Collaborators: Prof. Banafshe Larijani, Dr Lucy Collinson, Prof. Paul Verkade, Prof. Dominic Poccia

Future Research

Funded PhD Studentships Available

Biological Mathematics (with Dr Reuben O'Dea): Blood Vessel Permeability

Check out the BBSRC Doctoral Training program

• DALWADI, MOHIT P., KING, JOHN R., DYSON, ROSEMARY J. and ARKILL, KENTON P., 2020. Mathematical model to determine the effect of a sub-glycocalyx space PHYSICAL REVIEW FLUIDS. 5(4),
• ALLEN, CLAIRE L, MALHI, NASEEB K, WHATMORE, JAQUELINE D, BATES, DAVID O and ARKILL, KENTON P, 2020. Non-invasive measurement of retinal permeability in a diabetic rat model. Microcirculation (New York, N.Y. : 1994).
• OWEN-WOODS, CHARLOTTE, JOULIA, REGIS, BARKAWAY, ANNA, ROLAS, LOIC, MA, BIN, NOTTEBAUM, ASTRID FEE, ARKILL, KENTON P, STEIN, MONJA, GIRBL, TAMARA, GOLDING, MATTHEW, BATES, DAVID O, VESTWEBER, DIETMAR, VOISIN, MATHIEU-BENOIT and NOURSHARGH, SUSSAN, 2020. Local microvascular leakage promotes trafficking of activated neutrophils to remote organs. The Journal of clinical investigation. 130(5), 2301-2318
• ASHWORTH, J. C., THOMPSON, J. L., JAMES, J. R., SLATER, C. E., PIJUAN-GALITO, S., LIS-SLIMAK, K., HOLLEY, R. J., MEADE, K. A., THOMPSON, A., ARKILL, K. P., TASSIERI, M., WRIGHT, A. J., FARNIE, G. and MERRY, C. L. R., 2020. Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro MATRIX BIOLOGY. 85-86, 15-33
• CHUNG, GARY HONG CHUN, DOMART, MARIE-CHARLOTTE, PEDDIE, CHRISTOPHER, MANTELL, JUDITH, MCLAVERTY, KIERAN, ARABIOTORRE, ANGELA, HODGSON, LORNA, BYRNE, RICHARD D, VERKADE, PAUL, ARKILL, KENTON, COLLINSON, LUCY M and LARIJANI, BANAFSHE, 2018. cis-Golgi affects localized nuclear envelope morphology during mitosis. Journal of lipid research. 59(8), 1402-1413
• NEAL, CHRISTOPHER R., ARKILL, KENTON P., BELL, JAMES S., BETTERIDGE, KAI B., BATES, DAVID O., WINLOVE, C. PETER, SALMON, ANDREW H. J. and HARPER, STEVEN J., 2018. Novel hemodynamic structures in the human glomerulus AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY. 315(5), F1370-F1384
• TRIDGETT, MATTHEW, LOZANO, LUCIA, PASSARETTI, PAOLO, DESAI, NIMAI R., PROCTOR, TOBY J., LITTLE, HAYDN A., LOGAN, RICHARD T., ARKILL, KENTON P., OPPENHEIMER, POLA GOLDBERG and DAFFORN, TIMOTHY R., 2018. Dye Aggregate-Mediated Self-Assembly of Bacteriophage Bioconjugates BIOCONJUGATE CHEMISTRY. 29(11), 3705-3714
• MERRIFIELD RC, ARKILL KP, PALMER RE and LEAD JR, 2017. A High Resolution Study of Dynamic Changes of Ce2O3 and CeO2 Nanoparticles in Complex Environmental Media. Environmental science & technology. 51(14), 8010-8016
• BETTERIDGE KB, ARKILL KP, NEAL CR, HARPER SJ, FOSTER RR, SATCHELL SC, BATES DO and SALMON AHJ, 2017. Sialic acids regulate microvessel permeability, revealed by novel in vivo studies of endothelial glycocalyx structure and function. The Journal of physiology. 595(15), 5015-5035
• GALLAGHER MT, NEAL CV, ARKILL KP and SMITH DJ, 2017. Model-based image analysis of a tethered Brownian fibre for shear stress sensing J. R. Soc Interface. 14:20170564
• MICHEL, C. CHARLES, ARKILL, KENTON P. and CURRY, FITZROY E., 2016. The Revised Starling Principle and Its Relevance to Perioperative Fluid Management. In: FARAG, EHAB and KURZ, ANDREA, eds., Perioperative Fluid Management Springer International Publishing. 31-74
• CURRY, FITZROY E., ARKILL, KENTON P. and MICHEL, C. CHARLES, 2016. The Functions of Endothelial Glycocalyx and Their Effects on Patient Outcomes During the Perioperative Period. A Review of Current Methods to Evaluate Structure-Function Relations in the Glycocalyx in Both Basic Research and Clinical Settings. In: FARAG, EHAB and KURZ, ANDREA, eds., Perioperative Fluid Management Springer International Publishing. 75-116
• DOGRA, YUKTEE, ARKILL, KENTON P., ELGY, CHRISTINE, STOLPE, BJORN, LEAD, JAMIE, VALSAMI-JONES, EUGENIA, TYLER, CHARLES R. and GALLOWAY, TAMARA S., 2016. Cerium oxide nanoparticles induce oxidative stress in the sediment-dwelling amphipod Corophium volutator NANOTOXICOLOGY. 10(4), 480-487
• OLTEAN S, QIU Y, FERGUSON JK, STEVENS M, NEAL C, RUSSELL A, KAURA A, ARKILL KP, HARRIS K, SYMONDS C, LACEY K, WIJEYARATNE L, GAMMONS M, WYLIE E, HULSE RP, ALSOP C, COPE G, DAMODARAN G, BETTERIDGE KB, RAMNATH R, SATCHELL SC, FOSTER RR, BALLMER-HOFER K, DONALDSON LF, BARRATT J, BAELDE HJ, HARPER SJ, BATES DO and SALMON AHJ, 2015. Vascular Endothelial Growth Factor-A165b Is Protective and Restores Endothelial Glycocalyx in Diabetic Nephropathy. Journal of the American Society of Nephrology : JASN. 26(8), 1889-1904
• BAALOUSHA, M., ARKILL, K. P., ROMER, I., PALMER, R. E. and LEAD, J. R., 2015. Transformations of citrate and Tween coated silver nanoparticles reacted with Na2S SCIENCE OF THE TOTAL ENVIRONMENT. 502, 344-353
• ARKILL KP, MANTELL JM, PLANT SR, VERKADE P and PALMER RE, 2015. Using size-selected gold clusters on graphene oxide films to aid cryo-transmission electron tomography alignment. Scientific reports. 5, 9234
• PAYNE, L, ZORINIANTS, G, MASIA, F, ARKILL, KP, VERKADE, P, ROWLES, D, LANGBEIN, W and BORRI, P, 2015. Optical micro-spectroscopy of single metallic nanoparticles: quantitative extinction and transient resonant four-wave mixing: Faraday discussions Faraday discussions.
• LOBO, DANIELA P, WEMYSS, ALAN M, SMITH, DAVID J, STRAUBE, ANNE, BETTERIDGE, KAI B, SALMON, ANDREW HJ, FOSTER, REBECCA R, ELHEGNI, HESHAM E, SATCHELL, SIMON C, LITTLE, HAYDN A, PACHECO-GÓMEZ, RAÚL, SIMMONS, MARK J, HICKS, MATHEW R, BATES, DAVID O, RODGER, ALISON, DAFFORN, TIMOTHY R and ARKILL, KENTON P, 2015. Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle: Nano Research Nano Research. 8(10), 3307-3315
• ARKILL, KENTON P., QVORTRUP, KLAUS, STARBORG, TOBIAS, MANTELL, JUDITH M., KNUPP, CARLO, MICHEL, C. CHARLES, HARPER, STEVE J., SALMON, ANDY HJ, SQUIRE, JOHN M., BATES, DAVE O. and NEAL, CHRIS R., 2014. Resolution of the three dimensional structure of components of the glomerular filtration barrier BMC NEPHROLOGY. 15,
• CUDDY, MARTIN J., ARKILL, KENTON P., WANG, ZHI WEI, KOMSA, HANNU-PEKKA, KRASHENINNIKOV, ARKADY V. and PALMER, RICHARD E., 2014. Fabrication and atomic structure of size-selected, layered MoS2 clusters for catalysis NANOSCALE. 6(21), 12463-12469
• SCHULTE, KEVIN, BERGER, KATJA, BOOR, PETER, JIRAK, PEGGY, GELMAN, IRWIN H., ARKILL, KENTON P., NEAL, CHRISTOPHER R., KRIZ, WILHELM, FLOEGE, JUERGEN, SMEETS, BART and MOELLER, MARCUS J., 2014. Origin of Parietal Podocytes in Atubular Glomeruli Mapped by Lineage Tracing JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY. 25(1), 129-141
• ARKILL, KENTON P., NEAL, CHRIS R., MANTELL, JUDITH M., MICHEL, CHARLES C., QVORTRUP, KLAUS, ROSTGAARD, JORGEN, BATES, DAVE O., KNUPP, CARLO and SQUIRE, JOHN M., 2012. 3D Reconstruction of the Glycocalyx Structure in Mammalian Capillaries using Electron Tomography MICROCIRCULATION. 19(4), 343-351
• MANTELL, JUDITH M., VERKADE, PAUL and ARKILL, KENTON P., 2012. Quantitative biological measurement in Transmission Electron Tomography ELECTRON MICROSCOPY AND ANALYSIS GROUP CONFERENCE 2011 (EMAG 2011). 371,
• ARKILL, K. P., KNUPP, C., MICHEL, C. C., NEAL, C. R., QVORTRUP, K., ROSTGAARD, J. and SQUIRE, J. M., 2011. Similar Endothelial Glycocalyx Structures in Microvessels from a Range of Mammalian Tissues: Evidence for a Common Filtering Mechanism? BIOPHYSICAL JOURNAL. 101(5), 1046-1056
• ARKILL, KENTON P., MOGER, JULIAN and WINLOVE, C. PETER, 2010. The structure and mechanical properties of collecting lymphatic vessels: an investigation using multimodal nonlinear microscopy JOURNAL OF ANATOMY. 216(5), 547-555
• MOGER, C. J., ARKILL, K. P., BARRETT, R., BLEUET, P., ELLIS, R. E., GREEN, E. M. and WINLOVE, C. P., 2009. Cartilage Collagen Matrix Reorientation and Displacement in Response to Surface Loading JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME. 131(3),
• MACDONALD, A. J., ARKILL, K. P., TABOR, G. R., MCHALE, N. G. and WINLOVE, C. P., 2008. Modeling flow in collecting lymphatic vessels: one-dimensional flow through a series of contractile elements AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY. 295(1), H305-H313
• ARKILL, K. P. and WINLOVE, C. P., 2008. Solute transport in the deep and calcified zones of articular cartilage OSTEOARTHRITIS AND CARTILAGE. 16(6), 708-714
• MUTHUVELU, P., ELLIS, R. E., GREEN, E. M., ATTENBURROW, D., BARRETT, R., ARKILL, K., COLRIDGE, D. B., WINLOVE, C. P. and BRADLEY, D. A., 2007. Microvascularity, blood flow and tissue structure at the subchondral plate using an X-ray fluorescence technique JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY. 271(3), 771-775
• BRADLEY, D. A., MUTHUVELU, P., ELLIS, R. E., GREEN, E. M., ATTENBURROW, D., BARRETT, R., ARKILL, K., COLRIDGE, D. B. and WINLOVE, C. P., 2007. Characterisation of mineralisation of bone and cartilage: X-ray diffraction and Ca and SrK alpha X-ray fluorescence microscopy NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS. 263(1), 1-6
• ARKILL, KENTON PAUL and WINLOVE, C. PETER, 2006. Fatty acid transport in articular cartilage ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 456(1), 71-78