Facilities at the BBSRC Nottingham Wheat Research Centre
The BBSRC Nottingham Wheat Research Centre has three purpose built glasshouses which have temperature, light, and irrigation control. These were named the Gustafson glasshouses after Perry Gustafson at the opening of the Nottingham BBSRC Wheat Research Centre in 2016 with Jeanie Borlaug.
A purpose built seed store at the BBSRC Nottingham Wheat Research Centre from Pitkin and Ruddock with temperature and humidity control.
Zeiss Axio Imager Z2 upright epifluorescence microscope
The Zeiss Axio Imager microscope (Carl Zeiss Ltd, Oberkochen, Germany), is used for analysis of chromosome spreads which visualise our introgressed segments using either Genomic in situ hybridisation (GISH) or Fluorescent in situ hybridisation (FISH) techniques. The microscope has a MetaSystems Coolcube 1m CCD camera attached for photographs and slides can be further analysed using Meta Systems ISIS and Metafer software (Metasystems GmbH, Altlussheim, Germany).
The SNP genotyping pipeline allows the screening of lines for introgressions from the wild relatives. The pipeline enables;
- Automatic liquid handling
- Thermal cycling (PCR machines)
- Detection and data analysis (qPCR)