John Brameld

Contact

• workRoom 43 North Lab Bldg
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6133
• fax0115 951 6122
• john.brameld@nottingham.ac.uk

Teaching Summary

I am Module convenor for D224N2 (Principles of Human Nutrition), D224N3 (Principles of Animal Nutrition) and D23BN2 (Animal Nutrition).

I teach on the following modules throughout the 3 years of the BSc courses:

1. D23BN1 - Introduction to Nutrition
2. D22BN1 - Nutrition, Metabolism & Disease
3. D224N2 - Principles of Human Nutrition
4. D224N3 - Principles of Animal Nutrition
5. D23BN2 - Animal Nutrition
6. D23BN3 - Molecular Nutrition

I was the lead co-ordinator for the development of the E-Learning package, the Vitamin Village (http://www.nottingham.ac.uk/~cczcal/VitaminVillage/)

Research Summary

My Research interests are predominantly the study of interactions between hormones and nutrients in the regulation of growth, development and metabolism, particularly of liver, skeletal muscle and… read more

Selected Publications

• GRAHAM N.S, MAY S.T, DANIEL Z.C.T.R, EMMERSON Z.F, BRAMELD J.M and PARR T., 2011. Use of the Affymetrix Human GeneChip array and Genomic DNA hybridisation probe selection to study ovine transcriptomes Animal. 5(6), 861-866
• BRAMELD, J M, HOLD, R and BROUGHTON PIPKIN, F, 2011. Regional variation in angiotensin converting enzyme activity in the human placenta. Placenta. 32(11), 906-908
• MURPHY M, JETHWA PH, WARNER A, BARRETT P, NILAWEERA KN, BRAMELD JM and EBLING FJP, 2012. Effects Of Manipulating Hypothalamic Triiodothyronine Concentrations On Seasonal Body Weight And Torpor Cycles In Siberian Hamsters. Endocrinology. 153(1), 101-112
• BROWN DM, PARR T and BRAMELD JM, 2012. Myosin Heavy Chain Mrna Isoforms Are Expressed In Two Distinct Cohorts During C2c12 Myogenesis. Journal Of Muscle Research And Cell Motility. (In Press.)

• View all publications

Current Research

My Research interests are predominantly the study of interactions between hormones and nutrients in the regulation of growth, development and metabolism, particularly of liver, skeletal muscle and adipose tissue. I previously spent some time in the pharmaceutical industry and this has influenced the direction of my studies, which fall into 3 broad areas:

1) Nutrient-gene interactions.

These are all cell culture-based studies investigating the mechanisms by which specific nutrients influence gene expression.

• Past studies investigated the effects of glucose and amino acids on expression of genes of the growth hormone-insulin like growth factor (GH-IGF) axis. We demonstrated interactions between various hormones (insulin, thyroid hormones, glucocorticoids and growth hormone) and specific nutrients (glucose and individual amino acids) in the regulation of expression of growth hormone receptor (GHR) and IGF-I mRNA using cultured pig hepatocytes.
• Other studies in this area include i) effects of fatty acids on expression of key fat metabolism genes (e.g. stearoyl coA desaturase, SCD); ii) effects of plant-derived polyphenols (e.g. flavonoids) on vascular endothelial cell growth and gene expression, including microarray analyses; and iii) effects of fatty acids and oxidants/antioxidants on proliferation and differentiation of cultured muscle cells.
• More recent studies are investigating the effects of nuclear hormones/vitamins (e.g. vitamin A and D) on differentiation of muscle cells, including the trans-differentiation to other cell types (e.g. adipose and bone).

2) Development of body composition.

The GH-IGF axis plays a role in regulating the growth and differentiation of virtually all cell types, but particularly skeletal muscle and adipose tissue. The effects of nutrients on the GH-IGF axis may be particularly important during fetal life when skeletal myoblast and preadipocyte determination, proliferation and/or differentiation takes place.

• Past studies investigated the effects of maternal nutrition on muscle and fat cell growth and development, and the long-term consequences for growth, development and metabolism of the offspring.
• Of particular interest has been the effect of maternal nutrition on muscle fibre number and fibre type composition of the offspring, using molecular and histochemical methodologies in a variety of animal model systems.
• This has recently been extended to investigate the regulation of skeletal muscle fibre type transitions during postnatal development, using a combined transcriptomic and metabolomic approach. This then relates to the next area.

3) Energy balance.

The regulation of energy balance (intake vs expenditure) and nutrient partitioning is important both in terms of farm animals providing food, but also humans developing obesity and associated co-morbidities (insulin resistance, hypertension, etc).

• In vitro studies include the effects of hormones and nutrients (e.g. glucose and polyphenols) on fat breakdown (lipolysis) using cultured fat explants from pigs, rats and humans.
• In vivo studies are investigating the regulation of appetite (via hypothalamic neuropeptides such as TRH, AgRP and VGF) and whole body energy expenditure (oxygen consumption) using closed- and open-circuit calorimeters (e.g. CLAMS), including the effects of dietary polyphenols and plant extracts. The role of skeletal muscle characteristics (e.g. fibre number and type) in whole body energy expenditure is of particular interest, including the peripheral and central effects of nuclear hormone receptors and associated ligands (e.g. thyroid hormone, vitamin A and D and PPARs).

• MURPHY M, JETHWA PH, WARNER A, BARRETT P, NILAWEERA KN, BRAMELD JM and EBLING FJP, 2012. Effects Of Manipulating Hypothalamic Triiodothyronine Concentrations On Seasonal Body Weight And Torpor Cycles In Siberian Hamsters. Endocrinology. 153(1), 101-112
• BROWN DM, PARR T and BRAMELD JM, 2012. Myosin Heavy Chain Mrna Isoforms Are Expressed In Two Distinct Cohorts During C2c12 Myogenesis. Journal Of Muscle Research And Cell Motility. (In Press.)
• GRAHAM N.S, MAY S.T, DANIEL Z.C.T.R, EMMERSON Z.F, BRAMELD J.M and PARR T., 2011. Use of the Affymetrix Human GeneChip array and Genomic DNA hybridisation probe selection to study ovine transcriptomes Animal. 5(6), 861-866
• BRAMELD, J M, HOLD, R and BROUGHTON PIPKIN, F, 2011. Regional variation in angiotensin converting enzyme activity in the human placenta. Placenta. 32(11), 906-908
• REHFELDT, C, PAS, MFWT, WIMMERS, K, BRAMELD, JM, NISSEN, PM, BERRI, C, VALENTE, LMP, POWER, DM, PICARD, B, STICKLAND, NC and OKSBJERG, N, 2011. Advances In Research On The Prenatal Development Of Skeletal Muscle In Animals In Relation To The Quality Of Muscle-Based Food. I. Regulation Of Myogenesis And Environmental Impact Animal. 5(5), 703-717
• REHFELDT, C, PAS, MFWT, WIMMERS, K, BRAMELD, JM, NISSEN, PM, BERRI, C, VALENTE, LMP, POWER, DM, PICARD, B, STICKLAND, NC and OKSBJERG, N, 2011. Advances In Research On The Prenatal Development Of Skeletal Muscle In Animals In Relation To The Quality Of Muscle-Based Food. Ii - Genetic Factors Related To Animal Performance And Advances In Methodology Animal. 5(5), 718-730
• NICHOLSON, SK, TUCKER, GA and BRAMELD, JM, 2010. Physiological concentrations of dietary polyphenols regulate vascular endothelial cell expression of genes important in cardiovascular health. British Journal of Nutrition. 103, 1398-1403
• WARNER, AMY, JETHWA, PREETI H, WYSE, CATHERINE A, I'ANSON, HELEN, BRAMELD, JOHN M and EBLING, FRANCIS J P, 2010. Effects of photoperiod on daily locomotor activity, energy expenditure and feeding behavior in a seasonal mammal. American Journal of Physiology. Regulatory, integrative and comparative physiology. 298, R1409-R1416
• JETHWA, PREETI H, WARNER, AMY, FOWLER, MAXINE J, MURPHY, MICHELLE, DE BACKER, MARIJKE W, ADAN, ROGER A H, BARRETT, PERRY, BRAMELD, JOHN M and EBLING, FRANCIS J P, 2010. Short-days induce weight loss in Siberian hamsters despite overexpression of the agouti-related peptide (AgRP) gene. Journal of Neuroendocrinology. 22, 564-575
• ZULKIFLI, R M, PARR, T, SALTER, A M and BRAMELD, J M, 2010. Regulation of ovine and porcine stearoyl coenzyme A desaturase gene promoters by fatty acids and sterols. Journal of animal science. 88(8), 2565-75
• JETHWA, PH, WARNER, A, FOWLER, MJ, MURPHY, M, DE BACKER, MW, ADAN, RAH, BARRETT, P, BRAMELD, JM and EBLING, FJP, 2010. Short-Days Induce Weight Loss In Siberian Hamsters Despite Overexpression Of The Agouti-Related Peptide Gene Journal Of Neuroendocrinology. 22(6), 564-575
• WARNER, A, JETHWA, PH, WYSE, CA, I'ANSON, H, BRAMELD, JM and EBLING, FJP, 2010. Effects Of Photoperiod On Daily Locomotor Activity, Energy Expenditure, And Feeding Behavior In A Seasonal Mammal American Journal Of Physiology-Regulatory Integrative And Comparative Physiology. 298(5), R1409-R1416
• HEMMINGS, KM, PARR, T, DANIEL, ZCTR, PICARD, B, BUTTERY, PJ and BRAMELD, JM, 2009. Examination of myosin heavy chain isoform expression in ovine skeletal muscles. Journal of animal science. 87(12), 3915-3922
• HEMMINGS, KM, PARR, T, DANIEL, ZCTR, PICARD, B, BUTTERY, PJ and BRAMELD, JM, 2009. Examination Of Myosin Heavy Chain Isoform Expression In Ovine Skeletal Muscles Journal Of Animal Science. 87(12), 3915-3922
• NICHOLSON, SONJA K, TUCKER, GREGORY A and BRAMELD, JOHN M, 2008. Effects of dietary polyphenols on gene expression in human vascular endothelial cells. Proceedings of the Nutrition Society. 67(1), 42-7
• NICHOLSON, S.K., TUCKER, G.A. and BRAMELD, J.M., 2008. Effects of dietary polyphenols on gene expression in human vascular endothelial cells Proceedings of the Nutrition Society. 67, 42-47
• BRAMELD, J.M. and DANIEL, Z.C.T.R., 2008. In utero effects on livestock muscle development and body composition Australian Journal of Experimental Agriculture. 48(6-7), 921-929
• BRAMELD, JM and DANIEL, ZCTR, 2008. In Utero Effects On Livestock Muscle Development And Body Composition Australian Journal of Experimental Agriculture. 48(7), 921-929
• MALLINSON, J.E., SCULLEY, D.V., CRAIGON, J., PLANT, R., LANGLEY-EVANS, S.C. and BRAMELD, J.M., 2007. Fetal exposure to a maternal low-protein diet during mid-gestation results in muscle-specific effects on fibre type composition in young rats British Journal of Nutrition. 98(2), 292-299
• JETHWA, P.H., WARNER, A., NILAWEERA, K.N., BRAMELD, J.M., KEYTE, J.W., CARTER, W.G., BOLTON, N., BRUGGRABER, M., MORGAN, P.J., BARRETT, P. and EBLING, F.J.P., 2007. VGF-derived peptide, TLQP-21, regulates food intake and body weight in Siberian hamsters Endocrinology. 148(8), 4044-4055
• SCHUHLER, S., WARNER, A., FINNEY, N, BENNETT, G.W., EBLING, F.J.P. and BRAMELD, J.M., 2007. Thyrotrophin-releasing hormone decreases feeding and increases body temperature, activity and oxygen consumption in Siberian hamsters. Journal of Neuroendocrinology. 19(4), 239-49
• DANIEL, ZCTR, BRAMELD, JM, CRAIGON, J, SCOLLAN, ND and BUTTERY, PJ, 2007. Effect Of Maternal Dietary Restriction During Pregnancy On Lamb Carcass Characteristics And Muscle Fiber Composition Journal Of Animal Science. 85(6), 1565-1576
• HURLEY, M.S., FLUX, C., SALTER, A.M. and BRAMELD, J.M., 2006. Effects of fatty acids on skeletal muscle cell differentiation in vitro British Journal of Nutrition. 95(3), 623-630
• BRAMELD, J., DANIEL, Z., FAHEY, A., PARR, T., SCOLLAN, N. and BUTTERY, P., 2006. Effect of maternal undernutrition on ruminant carcass and meat quality Archiv Für Tierzucht. 49(SPI), 25
• JETHWA, P. H., WARNER, A., BRAMELD, J. M., KEYTE, J. W., NILAWEERA, N. K., MORGAN, P. J., BARRETT, P. and EBLING, F. J., 2006. The role of a VGF derived peptide in the regulation of food intake in a seasonal rodent FRONTIERS IN NEUROENDOCRINOLOGY. VOL 27(NUMBER 1), 5-6
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2005. Ontogeny of factors associated with proliferation and differentiation of muscle in the ovine fetus Journal of Animal Science. 83(10), 2330-2338
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2005. The effect of maternal undernutrition before muscle differentiation on the muscle fiber development of the newborn lamb Journal of Animal Science. 83(11), 2564-2571
• PARR, T., JEWELL, K.K., SENSKY, P.L., BRAMELD, J.M., BARDSLEY, R.G. and BUTTERY, P.J., 2004. Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters Archives of Biochemistry and Biophysics. 427(1), 8-15
• BRAMELD, J. M., 2004. The influence of undernutrition on skeletal muscle development British Journal of Nutrition. VOL 91(PART 3), 327-328
• HEADLAND, K. R., SENSKY, P. L., BUTTERY, P. J., DICKINSON, K. and BRAMELD, J. M., 2004. Short- and long-term effects of growth hormone on basal and catecholamine-induced lipolysis in pigs PROCEEDINGS- NUTRITION SOCIETY OF LONDON. VOL 63(SPI/OCB), 100A
• SENSKY, P.L., BARDSLEY, R.G., BRAMELD, J.M., DANIEL, Z.C., FAHEY, A., JEWELL, K.K., LEE, G.K., PARR, T., SALTER, A.M., SAZILI, A.Q., BUTTERY, P.J., WISEMAN, J., VARLEY, M.A. and KEMP, B., 2003. The biochemical basis for aspects of meat quality. In: WISEMAN, J., VARLEY, M.A. and B. KEMP, eds., Perspectives in pig science Nottingham: Nottingham University Press. 133
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2003. The effect of reducing protein and energy intake of pregnant ewes on the subsequent muscle development of the offspring. In: SOUFFRANT, W.B. and METGES, C.C., eds., Progress in research on energy and protein metabolism Wageningen: Wageningen Academic Publishers. 151-154
• BRAMELD JM, FAHEY AJ, LANGLEY-EVANS SC, BUTTERY PJ, 2003. Nutritional and hormonal control of muscle growth and fat deposition Archives of Animal Breeding. 46, 143-156
• BRAMELD, J. M. and BUTTERY, P. J., 2003. Certain amino acids regulate growth hormone (GH) binding to cultured pig hepatocytes PUBLICATION- EUROPEAN ASSOCIATION FOR ANIMAL PRODUCTION. VOL 109, 761-764
• BISPHAM, J., DANDREA, J., MOSTYN, A., BRAMELD, J.M., BUTTERY, P.J., STEPHENSON, T. and SYMONDS, M.E., 2002. Impact of maternal nutrient restriction in early to mid gestation on leptin, insulin-like growth factor-1 (IGF-1) and growth hormone receptor (GHR) mRNA abundance in adipose tissue of the fetal lamb. Early Human Development. 68, 135
• BRAMELD, J. M. and BUTTERY, P. J., 2002. Regulation of growth hormone (GH) binding to cultured pig hepatocytes by certain amino acids PROCEEDINGS- NUTRITION SOCIETY OF LONDON. VOL 61(SPI/OCA), 48A
• BRAMELD, J.M., MOSTYN, A., DANDREA, J., STEPHENSON, T.J., DAWSON, J.M., BUTTERY, P.J. and SYMONDS, M.E., 2000. Maternal nutrition alters the expression of insulin-like growth factors in fetal sheep liver and skeletal muscle Journal of Endocrinology. 167(3), 429-37
• BUTTERY, P.J., BRAMELD, J.M. and DAWSON, J.M., 2000. Control and manipulation of hyperplasia and hypertrophy in muscle tissue. In: CRONJÉ, P. and BOOMKER, E.A., eds., Ruminant physiology: digestion, metabolism, growth and reproduction Wallingford: CABI Publishing. 237-254
• HEASMAN, L, BRAMELD, J, MOSTVN, A, BUDGE, H, DAWSON, J, BUTTERY, P, STEPHENSON, T and SYMONDS, M E, 2000. Maternal nutrient restriction during early to mid gestation alters the relationship between insulin-like growth factor I and bodyweight at term in fetal sheep. Reproduction, fertility, and development. 12(7-8), 345-50
• BRAMELD, J.M., GILMOUR, R.S. and BUTTERY, P.J., 1999. Glucose and amino acids interact with hormones to control expression of insulin-like growth factor-I and growth hormone receptor mRNA in cultured pig hepatocytes Journal of Nutrition. 129(7), 1298-1306
• BRAMELD, J.M., SMAIL, H., IMRAM, N., MILLARD, N. and BUTTERY, P.J., 1999. Changes in expression of IGF-I, IGF-II and GH-receptor (GHR) mRNA during differentiation of cultured muscle cells derived from adult and fetal sheep South African Journal of Animal Science. 29 (ISRP)(Addendum), 307-310
• TAYLOR, P.M. and BRAMELD, J.M., 1999. Mechanisms and regulation of transcription and translation (Invited Review). In: Protein metabolism and nutrition: Proceedings of the VIIIth International Symposium on Protein Metabolism and Nutrition 25-50
• BRAMELD, J.M., SENSKY, P.L., BUDD, T., HARPER, J.M., PARR, T., WARD, R., WRIGHT, M., BARDSLEY, R.G., DAWSON, J., SALTER, A.M. and BUTTERY, P.J., 1998. Protein and fat metabolism in muscle and adipose tissue: its influence on meat quality. In: WISEMAN, J., VARLEY, M.A. and CHADWICK, J.P., eds., Progress in pig science Nottingham: Nottingham University Press. 443-460
• BRAMELD, J.M., BUTTERY, P.J., DAWSON, J.M. and HARPER, J.M., 1998. Nutritional and hormonal control of skeletal-muscle cell growth and differentiation In: Proceedings of the Nutrition Society. 207-217
• BRAMELD, J.M. and BUTTERY, P.J., 1998. Effects of nutrients on the expression of IGF-1 and GH-receptor (GHR) mRNA by cultured pig hepatocytes II. Amino acids Biotechnology, Agronomy Society and Environment. 2, 44
• BRAMELD, J.M. and BUTTERY, P.J., 1998. Effects of nutrients on the expression of IGF-1 and GH-receptor (GHR) mRNA by cultured pig hepatocytes I. Glucose Biotechnology, Agronomy Society and Environment. 2, 44
• GUAN, X.F., BRAMELD, J.M. and BUTTERY, P.J., 1997. Effects of dietary protein levels and porcine growth hormone administration on plasma concentrations of insulin-like growth factor binding proteins in growing pigs In: Proceedings of the Nutrition Society. 168A
• GUAN, X.F., BRAMELD, J.M., TROTTIER, N.L. and BUTTERY, P.J., 1997. Nutritional and hormonal regulation of insulin-like growth factor binding protein production in cultured porcine hepatocytes Journal of Animal Science. 75(Supplement 1), 77
• BRAMELD, J.M., 1997. Molecular mechanisms involved in the nutritional and hormonal regulation of growth in pigs In: Proceedings of the Nutrition Society. 607-619
• BRAMELD, J.M., ATKINSON, J.L., BUDD, T.J., SAUNDERS, J.C., PELL, J.M., SALTER, A.M., GILMOUR, R.S. and BUTTERY, P.J., 1996. Expression of insulin-like growth factor-1 (IGF-1) and growth hormone-receptor (GHR) mRNA in liver, skeletal muscle and adipose tissue of different breeds of pig Animal Science. 62(3), 555-560
• BRAMELD, J.M., ATKINSON, J.L., SAUNDERS, J.C., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1996. Effects of growth hormone administration and dietary protein intake on insulin-like growth factor-I and growth hormone receptor mRNA expression in porcine liver, skeletal muscle and adipose tissue Journal of Animal Science. 74, 1832-1841
• BRAMELD, J.M., ATKINSON, J.L., BUDD, T.J., SAUNDERS, J.C., SALTER, A.M., GILMOUR, R.S. and BUTTERY, P.J., 1995. Expression of IGF-I and GH-receptor (GHR) mRNA in liver and skeletal muscle of different pig breeds Animal Science. 60, 561
• BUTTERY, P.J., BRAMELD, J.M. and DAWSON, J.M., 1995. Regulation of growth and carcass composition in farm animals In: Proceedings of the 2nd Dummerstorf muscle workshop (Rostock, Germany). 175-182
• BRAMELD, J.M., WELLER, P.A., SAUNDERS, J.C., BUTTERY, P.J. and GILMOUR, R.S., 1995. Hormonal control of insulin-like growth factor-1 and growth hormone receptor mRNA expression by porcine hepatocytes in culture Journal of Endocrinology. 146, 239-245
• BRAMELD, J.M., WELLER, P.A., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1995. Ontogenic study of insulin-like growth factor-1 and growth hormone receptor mRNA expression in porcine liver and skeletal muscle Animal Science. 61, 333-339
• BRAMELD, J.M., ATKINSON, J.L., SAUNDERS, J.C., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1995. Effects of GH administration and dietary protein intake on IGF-I and GH-receptor (GHR),mRNA expression in pigs (Abstract) In: Proceedings of the 2nd Dummerstorf muscle workshop (Rostock, Germany).
• WELLER, P.A., DAUNCEY, M.J., BATES, P.C., BRAMELD, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1994. Regulation of porcine insulin-like growth-factor-1 and growth hormone receptor-RNA expression by energy status American Journal of Physiology. 266, E776-E785
• BLACHOWSKI, S., BUTTERY, P.J., BRAMELD, J., CHARLTON, D. and MOYTL, T., 1994. Inhibition of proliferation and differentiation of fetal sheep myoblasts by an inhibitor of ornithine decarboxylase-DFMO In: Proceedings of the Society of Nutrition Physiology. 291
• BLACHOWSKI, S., CHARLTON, D., BRAMELD, J., BUTTERY, P.J. and MOTYL, T., 1994. Comparison of metabolic effects of EGF, IGF-I, IGF-II and insulin in culture of fetal sheep myoblasts and fetal sheep fibroblasts. In: The developing of digestive and metabolic processes in new born and growing ruminants 69-70
• BRAMELD, J.M., WELLER, P.A., GILMOUR, R.S. and BUTTERY, P.J., 1993. Ontogenic study of expression of IGF-I and GH-receptor (GHR) mRNA in pig liver and skeletal muscle Animal Production. 56, 457
• BRAMELD, J M, BROUGHTON PIPKIN, F and SYMONDS, E M, 1990. Studies on the human ovarian renin-angiotensin system: optimization of assay methodology and effects of follicular stimulants. Journal of Endocrinology. 127(3), 513-21
• HESSLEWOOD, S R, THORNBACK, J R and BRAMELD, J M, 1988. Indium-111 in breast milk following administration of indium-111-labeled leukocytes. The Journal of Nuclear Medicine. 29(7), 1301-2