Michael Holdsworth

Contact

• workRoom 301B South Lab
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6046
• fax0115 951 6060
• michael.holdsworth@nottingham.ac.uk
• http://www.mycib.ac.uk/zope/holdsworth_lab

Research Summary

Current research programmes, recent publications etc are available on the Portal for the laboratory of Professor Holdsworth: HERE

Research programmes focus on:

• The role of the N-end rule pathway of targeted proteolysis in the control of plant growth and development.
• The function of the hormone Abscisic Acid (ABA), and plant responses to abiotic stress.

• Understanding the control of seed germination and seedling establishment.

• Systems approaches, integrating genetics, post-genomics, physiology, mathematics, computer science and engineering, are being used to understand and manipulate plant development and environmental responses.

• Emphasis is placed on the transfer of molecular genetic information from studies in model species such as Arabidopsis and rice, to address important agricultural problems associated with plant developmental biology and response to abiotic stress.

vSEED Portal: A Portal for the ERANET-PG-funded systems biology of seed germination/dormancy is here: http://vseed.eu/

Teaching interests: Current Issues in Crop Science - Genetic Improvement of Crop Plants - Plant Developmental Genetics - Brewing

Selected Publications

• GIBBS DJ, LEE SC, MD ISA N, GRAMUGLIA S, FUKAO T, BASSEL GW, CORREIA CS, CORBINEAU F, THEODOULOU FL, BAILEY-SERRES J and HOLDSWORTH MJ, 2011. Homeostatic Response To Hypoxia Is Regulated By The N-End Rule Pathway In Plants. Nature. 479, 415-418
• HOLMAN, T. J, JONES, P. D, RUSSELL, L, MEDHURST, A, UBEDA TOMÁS, S, TALLOJI, P, MARQUEZ, J, SCHMUTHS, H and ..... HOLDSWORTH, M. J., 2009. The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis. Proc Nat Acad Sci USA. 106(11), 4549-54
• BASSEL, G W, LAN, H, GLAAB, E, GIBBS, D J, GERJETS, T, KRASNOGOR, N, BONNER, A J, HOLDSWORTH, M J and PROVART, N J, 2011. From the Cover: Genome-wide network model capturing seed germination reveals coordinated regulation of plant cellular phase transitions. Proc Nat Acad Sci USA. 108(23), 9709-14
• BASSEL, G.W, GLAAB, E, MARQUEZ, J, HOLDSWORTH, M.J and BACARDIT, J., 2011. Functional Network Construction in Arabidopsis Using Rule-Based Machine Learning on Large-Scale Data Sets. The Plant Cell. 23, 3101-3116

• View all publications

• BAILEY-SERRES J, FUKAO T, GIBBS DJ, HOLDSWORTH MJ, LEE SC, LICAUSI F, PERATA P, VOESENEK LACJ and VAN DONGEN JT, 2012. Making Sense Of Low Oxygen Sensing. Trends In Plant Science. 17, 129-138
• LUCAS, M, SWARUP, R, PAPONOV, I A, SWARUP, K, CASIMIRO, I, LAKE, D, PERET, B, ZAPPALA, S, MAIRHOFER, S, WHITWORTH, M, WANG, J, LJUNG, K and ET AL, 2011. Short-Root regulates primary, lateral, and adventitious root development in Arabidopsis. Plant Physiology. 155(1), 384-98
• BASSEL, G W, LAN, H, GLAAB, E, GIBBS, D J, GERJETS, T, KRASNOGOR, N, BONNER, A J, HOLDSWORTH, M J and PROVART, N J, 2011. From the Cover: Genome-wide network model capturing seed germination reveals coordinated regulation of plant cellular phase transitions. Proc Nat Acad Sci USA. 108(23), 9709-14
• BASSEL, G. W, HOLDSWORTH, M.J and PROVART, N.J., 2011. Seed bioinformatics. Methods in molecular biology (Clifton, N.J.). 773, 403-19
• BASSEL, G.W, GLAAB, E, MARQUEZ, J, HOLDSWORTH, M.J and BACARDIT, J., 2011. Functional Network Construction in Arabidopsis Using Rule-Based Machine Learning on Large-Scale Data Sets. The Plant Cell. 23, 3101-3116
• DUPEUX, F, SANTIAGO, J, BETZ, K, TWYCROSS, J, PARK, S-Y, RODRIGUEZ, L, GONZALEZ-GUZMAN, M, JENSEN, M, KRASNOGOR, N, HOLDSWORTH, M.J, BLACKLEDGE, M, CUTLER, S and RODRIGUEZ P. AND MARQUEZ, J., 2011. A thermodynamic switch modulates abscisic acid receptor sensitivity. The EMBO journal. 30, 4171-4184
• GIBBS DJ, LEE SC, MD ISA N, GRAMUGLIA S, FUKAO T, BASSEL GW, CORREIA CS, CORBINEAU F, THEODOULOU FL, BAILEY-SERRES J and HOLDSWORTH MJ, 2011. Homeostatic Response To Hypoxia Is Regulated By The N-End Rule Pathway In Plants. Nature. 479, 415-418
• PEARCE, SP, KING, JR and HOLDSWORTH, MJ, 2011. Axisymmetric Indentation Of Curved Elastic Membranes By A Convex Rigid Indenter International Journal Of Non-Linear Mechanics. 46(9), 1128-1138
• GERJETS, T, SCHOLEFIELD, D, FOULKES, M J, LENTON, J R and HOLDSWORTH, M J, 2010. An analysis of dormancy, ABA responsiveness, after-ripening and pre-harvest sprouting in hexaploid wheat (Triticum aestivum L.) caryopses. Journal of Experimental Botany. 61(2), 597-607
• HOLMAN, T J, WILSON, M H, KENOBI, K, DRYDEN, I L, HODGMAN, T C, WOOD, A T and HOLDSWORTH, M J, 2010. Statistical evaluation of transcriptomic data generated using the Affymetrix one-cycle, two-cycle and IVT-Express RNA labelling protocols with the Arabidopsis ATH1 microarray. Plant methods. 6, 9
• DIETRICH, D, SCHMUTHS, H, DE MARCOS LOUSA, C, BALDWIN, J M, BALDWIN, S A, BAKER, A, THEODOULOU, F L and HOLDSWORTH, M J, 2009. Mutations in the Arabidopsis peroxisomal ABC transporter COMATOSE allow differentiation between multiple functions in planta: insights from an allelic series. Molecular Biology of the Cell. 20(1), 530-43
• HOLMAN, T. J, JONES, P. D, RUSSELL, L, MEDHURST, A, UBEDA TOMÁS, S, TALLOJI, P, MARQUEZ, J, SCHMUTHS, H and ..... HOLDSWORTH, M. J., 2009. The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis. Proc Nat Acad Sci USA. 106(11), 4549-54
• HOLDSWORTH, M J, BENTSINK, L and SOPPE, W J J, 2008. Molecular networks regulating Arabidopsis seed maturation, after-ripening, dormancy and germination. New Phytologist. 179(1), 33-54
• HOLDSWORTH, M J, FINCH-SAVAGE, W E, GRAPPIN, P and JOB, D, 2008. Post-genomics dissection of seed dormancy and germination. Trends in Plant Science. 13(1), 7-13
• CARRERA, E, HOLMAN, T, MEDHURST, A, DIETRICH, D, FOOTITT, S, THEODOULOU, F L and HOLDSWORTH, M J, 2008. Seed after-ripening is a discrete developmental pathway associated with specific gene networks in Arabidopsis. Plant Journal. 53(2), 214-24
• CARRERA, E., HOLMAN, T., MEDHURST, A., PEER, W., SCHMUTHS, H., FOOTITT, S., THEODOULOU, F.L. and HOLDSWORTH, M.J., 2007. Gene expression profiling reveals defined functions of the ATP-binding cassette transporter COMATOSE late in phase II of germination Plant Physiology. 143(4), 1669-79
• FOOTITT, S, DIETRICH, D, FAIT, A, FERNIE, A R, HOLDSWORTH, M J, BAKER, A and THEODOULOU, F L, 2007. The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis. Plant Physiology. 144(3), 1467-80
• HUNT, L, HOLDSWORTH, M J and GRAY, J E, 2007. Nicotinamidase activity is important for germination. Plant Journal. 51(3), 341-51
• BAUDO, M. M., LYONS, R., POWERS, S., PASTORI, G. M., EDWARDS, K. J., HOLDSWORTH, M. J. and SHEWRY, P. R., 2006. Transgenesis has less impact on the transcriptome of wheat grain than conventional breeding Plant Biotechnology Journal. VOL 4(NUMBER 4), 369-380
• FOOTITT, S, MARQUEZ, J, SCHMUTHS, H, BAKER, A, THEODOULOU, F L and HOLDSWORTH, M, 2006. Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis. Journal of Experimental Botany. 57(11), 2805-14
• MAYES, S., HOLDSWORTH, M.J., PELLEGRINESCHI, A. and REYNOLDS, M., 2005. Allying genetic and physiological innovations to improve productivity of wheat and other crops. In: Yields of farmed species - Constraints and Opportunities in the 21st century Nottingham University Press, Nottingham. 89 - 122
• EDWARDS, D., STEVENSON, D., FORSYTH, A., HEGARTY, M., BATLEY, J., HOLDSWORTH, M.J. and EDWARDS, K.J., 2004. Identification of transposon-tagged maize genes displaying homology to arrayed cDNA clones with the use of Mutator insertion display Genome. 1, 48-55
• FOOTITT, S., SLOCOMBE, S.P., LARNER, V., KURUP, S., WU, Y., LARSON, T., GRAHAM, I., BAKER, A. and HOLDSWORTH, M., 2002. Control of germination and lipid mobilisation by <i>COMATOSE</i>, the <i>Arabidopsis</i> homologue of human ALDP EMBO Journal. 21(12), 2912-2922
• MCKIBBIN, R.S., WILKINSON, M.D., BAILEY, P.C., FLINTHAM, J.E., ANDREW, L.M., LAZZERI, P.A., GALE, M.D., LENTON, J.R. and HOLDSWORTH, M.J., 2002. Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species Proceedings of the National Academy of Sciences of the United States of America. 99(15), 10203-8
• WILKINSON, M. D., MCKIBBIN, R. S., BAILEY, P. C., FLINTHAM, J. E., GALE, M. D., LENTON, J. R. and HOLDSWORTH, M. J., 2002. Use of comparative molecular genetics to study pre harvest sprouting in wheat Euphytica. VOL 126(NUMBER 1), 27 - 33
• PAZ-ARES, JAVIER and THE REGIA CONSORTIUM,, 2002. REGIA, An EU Project on Functional Genomics of Transcription Factors From Arabidopsis Thaliana. Comparative and functional genomics. 3(2), 102-8
• HEDDEN, P, HOLDSWORTH, M J, SIMPSON, G G, EDWARDS, K J and LENTON, J R, 2002. New frontiers in plant development: from genes to phenotype. Trends in Plant Science. 7(9), 381-3
• HOLDSWORTH, M.J., LENTON, J.R., FLINTHAM, J.E., GALE, M.D., KURUP, S., MCKIBBIN, R.S., BAILEY, P.C., LARNER, V. and RUSSELL, L., 2001. Genetic control mechanisms regulating the initiation of germination Journal of Plant Physiology. 158(4), 439
• RUSSELL, L, LARNER, V, KURUP, S, BOUGOURD, S and HOLDSWORTH, M, 2000. The Arabidopsis COMATOSE locus regulates germination potential. Development (Cambridge). 127(17), 3759-67
• KURUP, S, JONES, H D and HOLDSWORTH, M J, 2000. Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds. Plant Journal. 21(2), 143-55
• JONES, H D, KURUP, S, PETERS, N C and HOLDSWORTH, M J, 2000. Identification and analysis of proteins that interact with the Avena fatua homologue of the maize transcription factor VIVIPAROUS 1. Plant Journal. 21(2), 133-42
• BAILEY, P. C., MCKIBBIN, R. S., LENTON, J. R., HOLDSWORTH, M. J., FLINTHAM, J. E. and GALE, M. D., 1999. Genetic map locations for orthologous Vp1 genes in wheat and rice Theoretical and Applied Genetics. VOL 98(NUMBER 2), 281-284
• ALBANI, D, HAMMOND-KOSACK, M C, SMITH, C, CONLAN, S, COLOT, V, HOLDSWORTH, M and BEVAN, M W, 1997. The wheat transcriptional activator SPA: a seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes. Plant Cell. 9(2), 171-84
• HOLDSWORTH, M J, MUñOZ-BLANCO, J, HAMMOND-KOSACK, M, COLOT, V, SCHUCH, W and BEVAN, M W, 1995. The maize transcription factor Opaque-2 activates a wheat glutenin promoter in plant and yeast cells. Plant Molecular Biology. 29(4), 711-20
• GRIERSON, C, DU, J S, DE TORRES ZABALA, M, BEGGS, K, SMITH, C, HOLDSWORTH, M and BEVAN, M, 1994. Separate cis sequences and trans factors direct metabolic and developmental regulation of a potato tuber storage protein gene. Plant Journal. 5(6), 815-26
• BEVAN, M, COLOT, V, HAMMOND-KOSACK, M, HOLDSWORTH, M, TORRES DE ZABALA, M, SMITH, C, GRIERSON, C and BEGGS, K, 1993. Transcriptional control of plant storage protein genes. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences. 342(1301), 209-15
• HAMMOND-KOSACK, M C, HOLDSWORTH, M J and BEVAN, M W, 1993. In vivo footprinting of a low molecular weight glutenin gene (LMWG-1D1) in wheat endosperm. EMBO Journal. 12(2), 545-54
• HOLDSWORTH, M J, GRIERSON, C, SCHUCH, W and BEVAN, M, 1992. DNA-binding properties of cloned TATA-binding protein from potato tubers. Plant Molecular Biology. 19(3), 455-64
• HOLDSWORTH, M.J and G.G. LATIES, 1989. Site-specific binding of a nuclear factor to the carrot extensin gene is influenced by ethylene and wounding Planta. 179, 17-23
• HOLDSWORTH, M.J and G.G. LATIES, 1989. Identification of a wound-induced inhibitor of a nuclear factor that binds the carrot extensin gene Planta. 180, 74-81
• HOLDSWORTH, M J, SCHUCH, W and GRIERSON, D, 1987. Nucleotide sequence of an ethylene-related gene from tomato. Nucleic Acids Research. 15(24), 10600
• HOLDSWORTH, M J, BIRD, C R, RAY, J, SCHUCH, W and GRIERSON, D, 1987. Structure and expression of an ethylene-related mRNA from tomato. Nucleic Acids Research. 15(2), 731-9