Ioan Notingher

Contact

• workRoom B402 Physics
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 951 5172
• fax0115 951 5180
• ioan.notingher@nottingham.ac.uk

Research Summary

My research interest is the development of new optical and spectroscopic methods for studying biological materials at a nano and micro-scale. My main focus is the development of Raman spectroscopic… read more

Selected Publications

• PASCUT, FLAVIUS C, GOH, HUEY T, WELCH, NATHAN, BUTTERY, LEE D, DENNING, CHRIS and NOTINGHER, IOAN, 2011. Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells. Biophysical journal. 100(1), 251-9
• ZOLADEK, A, PASCUT, FC, PATEL, P and NOTINGHER I., 2011. Non-Invasive Time-Course Imaging of Apoptotic Cells by Confocal Raman Micro-Spectroscopy Journal of Raman Spectroscopy. 42, 251-258
• SWEETENHAM, CLAIRE S and NOTINGHER, IOAN, 2011. Combined AFM-Raman mapping of electric field enhancement and SERS hot-spots for nanosphere lithography substrates Journal of Nanophotonics. 5, 054101JNP (In Press.)
• PASCUT, FLAVIUS C, GOH, HUEY T, GEORGE, VINOJ, DENNING, CHRIS and NOTINGHER, IOAN, 2011. Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells. Journal of biomedical optics. 16(4), 045002

• View all publications

Current Research

My research interest is the development of new optical and spectroscopic methods for studying biological materials at a nano and micro-scale. My main focus is the development of Raman spectroscopic techniques for investigations of live cells, cell membranes and other cellular components. In Raman spectroscopy, a laser is used to interogate the sample at a molecular level and provide a "spectroscopic fingerprint" which is extremely rich in information regarding composition and structure of the biomolecules found in cells. The main advantage of this technique is that the information is obtained without need to damage cells or disturb any biological activities by using fluorescent labels or other contrast enhancing chemicals. Applications of these techniques range from understanding the interaction of cells with cancer drugs, toxicity assessment of nanomaterials, nanoscale imaging of lipid rafts, monitoring growth of engineered tissues and embryonic stem cells, .

For more details see the nanoscience web pages

Current Teaching

F33SO2 Laser Physics and Matter-Light Interactions

• PASCUT, FLAVIUS C, GOH, HUEY T, WELCH, NATHAN, BUTTERY, LEE D, DENNING, CHRIS and NOTINGHER, IOAN, 2011. Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells. Biophysical journal. 100(1), 251-9
• PASCUT, FLAVIUS C, GOH, HUEY T, GEORGE, VINOJ, DENNING, CHRIS and NOTINGHER, IOAN, 2011. Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells. Journal of biomedical optics. 16(4), 045002
• ZOLADEK, A, PASCUT, FC, PATEL, P and NOTINGHER I., 2011. Non-Invasive Time-Course Imaging of Apoptotic Cells by Confocal Raman Micro-Spectroscopy Journal of Raman Spectroscopy. 42, 251-258
• VERRIER, SOPHIE, ZOLADEK, ALINA and NOTINGHER, IOAN, 2011. Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test. Methods in molecular biology (Clifton, N.J.). 740, 179-89
• SWEETENHAM, CLAIRE S and NOTINGHER, IOAN, 2011. Combined AFM-Raman mapping of electric field enhancement and SERS hot-spots for nanosphere lithography substrates Journal of Nanophotonics. 5, 054101JNP (In Press.)
• ZOLADEK, ALINA BOGUMILA, JOHAL, RAMNEEK KAUR, GARCIA-NIETO, SAMUEL, PASCUT, FLAVIUS, SHAKESHEFF, KEVIN M, GHAEMMAGHAMI, AMIR M and NOTINGHER, IOAN, 2010. Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy. The Analyst. 135(12), 3205-12
• LEKPRASERT, BANYAT, SEDMAN, VICTORIA, ROBERTS, CLIVE J, TEDLER, SAUL J B and NOTINGHER, IOAN, 2010. Nondestructive Raman and atomic force microscopy measurement of molecular structure for individual diphenylalanine nanotubes. Optics letters. 35(24), 4193-5
• LARRAONA-PUY, MARTA, GHITA, ADRIAN, ZOLADEK, ALINA, PERKINS, WILLIAM, VARMA, SANDEEP, LEACH, IAIN H, KOLOYDENKO, ALEXEY A, WILLIAMS, HYWEL and NOTINGHER, IOAN, 2009. Development of Raman microspectroscopy for automated detection and imaging of basal cell carcinoma. Journal of biomedical optics. 14(5), 054031
• PAULIAC-VAUJOUR, E, STANNARD, A, MARTIN, C P, BLUNT, M O, NOTINGHER, I, MORIARTY, P J, VANCEA, I and THIELE, U, 2008. Fingering instabilities in dewetting nanofluids. Physical review letters. 100(17), 176102
• JELL, G, NOTINGHER, I, TSIGKOU, O, NOTINGHER, P, POLAK, J M, HENCH, L L and STEVENS, M M, 2008. Bioactive glass-induced osteoblast differentiation: a noninvasive spectroscopic study. Journal of biomedical materials research. Part A. 86(1), 31-40
• NOTINGHER,I. and HENCH,L.L., 2006. Raman microspectrocopy: a noninvasive tool for studies of individual living cells <i>in vitro</i> Expert Review of Medical Devices. 3(2), 215-234
• NOTINGHER, I. and ELFICK, A., 2005. Effect of sample and substrate electric properties on the electric field enhancement at the apex of SPM nanotips Journal of Physical Chemistry B. 109(33), 15699-15706
• NOTINGHER, I., JELL, G., NOTINGHER, P.L., BISSON, I., POLAK, J.M. and HENCH, L.L., 2005. Raman spectroscopy: Potential tool for in situ characterization of bone cell differentiation. In: Bioceramics, Vol 17 17.
• NOTINGHER, I., BISSON, I., BISHOP, A.E., RANDLE, W.L., POLAK, J.M.P. and HENCH, L.L., 2004. In situ spectral monitoring of mRNA translation in embryonic stem cells during differentiation in vitro Analytical Chemistry. 76(11), 3185-3193
• NOTINGHER, I., JELL, G., LOHBAUER, U., SALIH, V. and HENCH, L.L., 2004. In situ non-invasive spectral discrimination between bone cell phenotypes used in tissue engineering Journal of Cellular Biochemistry. 92(6), 1180-1192
• NOTINGHER, I. and IMHOF, R. E., 2004. Mid-infrared in vivo depth-profiling of topical chemicals on skin Skin Research And Technology. VOL 10(NUMBER 2), 113-121
• MAQUET, V., BOCCACCINI, A. R., PRAVATA, L., NOTINGHER, I. and JEROME, R., 2004. Porous poly(a-hydroxyacid)/Bioglass composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation Biomaterials. VOL 25(NUMBER 18), 4185-4194
• CLUPPER, D. C., GOUGH, J. E., EMBANGA, P. M., NOTINGHER, I., HENCH, L. L. and HALL, M. M., 2004. Bioactive evaluation of 45S5 bioactive glass fibres and preliminary study of human osteoblast attachment Journal of Materials Science. Materials in medicine. VOL 15(NUMBER 7), 803-808
• NOTINGHER, I, GREEN, C, DYER, C, PERKINS, E, HOPKINS, N, LINDSAY, C and HENCH, L L, 2004. Discrimination between ricin and sulphur mustard toxicity in vitro using Raman spectroscopy. Journal of the Royal Society, Interface / the Royal Society. 1(1), 79-90
• HENCH, L. L. and NOTINGHER, I., 2004. NovaTest: A Biophotonics System for Rapid In-Vitro Toxicity Testing American Ceramic Society Bulletin. VOL 83(PART 2), 14-15
• GOUGH, J. E., NOTINGHER, I. and HENCH, L. L., 2004. Osteoblast Attachment and Mineralized Nodule Formation on Rough and Smooth 45S5 Bioactive Glass Monoliths JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B. VOL 68(PART 4), 640-650
• NOTINGHER, I., GOUGH, J.E. and HENCH, L.L., 2004. Study of osteoblasts mineralisation in-vitro by Raman micro-spectroscopy. In: Bioceramics, Vol 16 254-2.
• NOTINGHER, IOAN, SELVAKUMARAN, JAMUNA and HENCH, LARRY L, 2004. New detection system for toxic agents based on continuous spectroscopic monitoring of living cells. Biosensors & bioelectronics. 20(4), 780-9
• VERRIER, S., NOTINGHER, I., POLAK, J. M. and HENCH, L. L., 2004. In Situ Monitoring of Cell Death Using Raman Microspectroscopy Biopolymers. VOL 74(NO 1-2), 157-162
• NOTINGHER, I., SELVAKUMARAN, J. and HENCH, L. L., 2004. New detection system for toxic agents based on continuous spectroscopic monitoring of living cells Biosensors & Bioelectronics. VOL 20(NUMBER 4), 780-789
• NOTINGHER, I., VERRIER, S., HAQUE, S., POLAK, J.M. and HENCH, L.L., 2003. Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells Biopolymers. 72(4), 230-240
• NOTINGHER, I., IMHOF, R. E., XIAO, P. and PASCUT, F. C., 2003. Spectral Depth Profiling of Arbitrary Surfaces by Thermal Emission Decay-Fourier Transform Infrared Spectroscopy Applied Spectroscopy. VOL 57(PART 12), 1494-1501
• BOCCACCINI, A. R., NOTINGHER, I., MAQUET, V. and JEROME, R., 2003. Bioresorbable and bioactive composite materials based on polylactide foams filled with and coated by Bioglass particles for tissue engineering applications Journal of Materials Science. Materials in medicine. VOL 14(NUMBER 5), 443-450
• NOTINGHER, I., IMHOF, R. E., XIAO, P. and PASCUT, F. C., 2003. Near-surface depth-resolved midinfrared emission spectroscopy Review of Scientific Instruments. VOL 74(NO 1), 346-348
• BLAKER, J. J., GOUGH, J. E., MAQUET, V., NOTINGHER, I. and BOCCACCINI, A. R., 2003. In Vitro Evaluation of Novel Bioactive Composites Based on Bioglassreg-Filled Polylactide Foams for Bone Tissue Engineering Scaffolds JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A. VOL 67(PART 4), 1401-1411
• NOTINGHER, I., JONES, J. R., VERRIER, S., BISSON, I., EMBANGA, P., EDWARDS, P., POLAK, J. M. and HENCH, L. L., 2003. Application of FTIR and Raman spectroscopy to characterisation of bioactive materials and living cells Spectroscopy-An International Journal. VOL 17(PART 2/3), 275-288
• MAQUET, V, BOCCACCINI, A R, PRAVATA, L, NOTINGHER, I and JéRôME, R, 2003. Preparation, characterization, and in vitro degradation of bioresorbable and bioactive composites based on Bioglass-filled polylactide foams. Journal of Biomedical Materials Research Part A. 66(2), 335-46
• NOTINGHER, I., VERRIER, S., ROMANSKA, H., BISHOP, A. E., POLAK, J. M. and HENCH, L. L., 2002. In situ characterisation of living cells by Raman spectroscopy Spectroscopy-An International Journal. VOL 16(PART 2), 43-52
• NOTINGHER, I., BOCCACCINI, A. R., JONES, J., MAQUET, V. and HENCH, L. L., 2002. Application of Raman microspectroscopy to the characterisation of bioactive materials Materials Characterization. VOL 49(NUMBER 3), 255-260
• NOTINGHER, I., XIAO, P., IMHOF, R.E., BERG, E.P. and PASCUT, F.C., 2001. New instrument for FT-thermal emission decay spectroscopy Analytical Sciences. 17, S486-S489
• BERG, E.P., XIAO, P., NOTINGHER, I., IMHOF, R.E. and ZOGG, H., 2001. Fast focal plane array detector and readout for pulsed opto-thermal radiometry Analytical Sciences. 17, S465-S468
• PASCUT, F.C., XIAO, P., O'DRISCOLL, D.G., KATZIR, A., GANNOT, I., CROITORU, N., NOTINGHER, I. and IMHOF, R.E., 2001. Fibre-optic hand held probe for opto-thermal in-vivo skin measurements Analytical Sciences. 17, S368-S370