Super Resolution Microscopy Facility
The Facility
The School of Life Sciences Imaging Facility (SLIM) has now installed a Zeiss PS1 Super Resolution Microscope resulting from a BBSRC Alert 13 award. The microscope comprises a Zeiss 780 high resolution confocal microscope combined with a Zeiss Super resolution system with full environmental control and is housed within a facility with level 2 containment. The system integrates high quality confocal microscopy with SIM, PALM, 3D PALM and STORM super resolution and is supporting a wide range of research projects across the School, University and collaborators from the Midlands M5 Universities. The microscope is an integral part of the state of the art imaging facilities within the School of Life Sciences that encompass wide field, confocal, live cell systems, TIRF, High Content Imaging, Multi Photon, FCS, EM and full preparation/histology suite. The system is managed By Tim Self who leads the SLIM imaging team and Robert Markus a Senior Imaging Technician dedicated to developing super resolution at the University of Nottingham.
Location
The Super Resolution Facility is housed in a category II suite within the Centre for Biomolecular Sciences (CBS) on the University Park Campus.
Location Map
Access
Access to the facility is open to researchers the University of Nottingham, outside institutions and industry. Data acquisition can be with Robert Markus/ Tim Self or following comprehensive training by the user alone. Advice and consultation on preparation and data analysis is available. Access charges and cost recovery model are now in place.
Data Storage
For University of Nottingham users all data will be stored centrally through OMERO to OME tiffs to a dedicated server. Zen analysis software can be accessed through remote desktop through client pcs. Plans for external access to OMERO and analysis are being explored.
Click here for information on Super Resolution Concepts
Click here for examples for current UoN research projects
Selected Recent Publications
Dunajová L, Cash E, Markus R, Rochette S, Townley AR, Wheatley SP. The NH2terminus of survivin is a mitochondrial targeting sequence and C-Src regulator. JCell Sci. 2016 May 31. pii: jcs.183277.
Willson BJ, Kovács K, Wilding-Steele T, Markus R, Winzer K, Minton NP. Production of a functional cell wall-anchored minicellulosome by recombinantClostridium acetobutylicum ATCC 824. Biotechnol Biofuels. 2016 May 23;9:109.
For more details and access to the system please contact:
Tim Self
Chief Experimental Officer
School of Life Sciences
Tim.self@nottingham.ac.uk
Tel: +44 115 82 30090
Robert Markus
Senior Imaging Technician
School of Life Sciences
Robert.Markus@nottingham.ac.uk
Tel: +44(0)1158467971
For Academic enquiries please contact:
Prof. Miguel Camara
Professor of Molecular Microbiology
School of Life Sciences
Miguel.camara@nottingham.ac.uk
Tel: +44 (0)115 9515036