2015 Elected Fellow of the Royal Society of Biology
2012-present: STFC Senior Molecular Biology and Neutron Fellow
2012-present: Group Leader in Biophysical Methods in the Research Complex at Harwell.
2011-present: Associate Professor and Reader in Physical Biochemistry.
2009-2011: Associate Professor in Physical Biochemistry, University of Nottingham and Deputy Director of the National Centre for Macromolecular Hydrodynamics, University of Nottingham. 2003-2009: Lecturer in Physical Biochemistry. University of Nottingham. 2001-2003: Post-doctoral research associate. Department of Biochemistry, University of Bristol. I worked for Prof. SteveHalford on a Wellcome funded programme grant to study the mechanism of Type II restriction enzymes that require one and two DNA binding sites. 2000-2001: Post-doctoral research assistant, Oxford Centre for Molecular Sciences, University of Oxford/YSBL, Department of Chemistry, University of York. For this year I worked 50 % of the time in the laboratory of Prof. Carol Robinson at Oxfordanalysing non-covalent macromolecular complex formation using nanospray mass spectrometry. The rest of the time I continued to work in the YSBL. 1999-2000: Post-doctoral research assistant, York Structural Biology Laboratory, Departments of Chemistry and of Biology, University of York. BBSRC funded position in the YSBL. I was involved in developing biophysical techniques such as analytical ultracentrifugation, isothermal titration calorimetry and small angle scattering. 1996-1999: Post-doctoral research assistant, Department of Biology, University of York. BBSRC 3 year project grant working for Dr. Jim Hoggett on interactions of sigmaN with E. coli RNA polymerase using novel tryptophan analogues.
Graduate and Undergraduate Studies
1992-1996: Postgraduate, Department of Biology, University of Leeds. PhD Thesis: "Kinetic analysis of bacteriophage MS2 self-assembly", supervised by Prof. Peter Stockley.
1989-1992: Undergraduate, Department of Biochemistry and Molecular Biology, University of Leeds. BSc Molecular Biophysics
Expertise in Analytical Ultracentrifugation, small angle X-ray scattering, small angle neutron scattering, isothermal titration calorimetry, protein expression and characterisation. Also experience of protein crystallography, cro-EM and NMR.
D24BT8 Structural Biology; D224G1 Employavility modeule. I also supervise final year Microbiology students in their 3rd year lab based projects.
I am currently seconded two days a week to the Research Complex at Harwell (www.rc-harwell.ac.uk) where I am Group Leader in Biophysical Methods. I hold an appointment as the ISIS Senior Molecular… read more
HYDE EI, CALLOW P, RAJASEKAR KV, TIMMINS P, PATEL TR, SILIGARDI G, HUSSAIN R, WHITE SA, THOMAS CM and SCOTT DJ, 2017. Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA. The Biochemical journal. 474(18), 3121-3135 ARENA DE SOUZA V, SCOTT DJ, NETTLESHIP JE, RAHMAN N, CHARLTON MH, WALSH MA and OWENS RJ, 2015. Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PloS one. 10(12), e0143919
GREEN, M., HATTER, L., BROOKES, E., SOULTANAS, P. and SCOTT, D.J., 2016. Defining the Intrinsically Disordered C-terminal Domain of SSB Reveals DNA-mediated Compaction Journal of Molecular Biology. 428, 357-364
I am currently seconded two days a week to the Research Complex at Harwell (www.rc-harwell.ac.uk) where I am Group Leader in Biophysical Methods. I hold an appointment as the ISIS Senior Molecular Biology and Neutron Fellow, sponsored by the STFC.
The Scott Group: We work on a variety of biophysical and biological problems centred around how organisms deal with and process biological information. Current projects include: Olfaction, intrinsic disorder in proteins, transcriptions and aggregate structure and formation in pharmaceutical preparations and development of methodologies to cope with non-ideal highly concentrated solutions.
Biophysical Techniques used: Analytical ultracentrifugation, isothermal titration calorimetry, light scattering, small angle X-ray and neutron scattering and hydrodynamic modelling.
Other techniques: Standard molecular biology for isolation and overexpression of target genes. RT-PCR, receptor analysis, single gene knockouts. We also have over-expression systems for yeast, archaea and bacteria.
We are, or have been funded by BBSRC, MRC, The Wellcome Trust, STFC, EPSRC, UNESCO, Royal Society and the University of Nottingham.
My current research is based around using multiple biophysical techniques in order to elucidate structure/function relationships. I am keenly interested in employing techniques such as small angle scattering, hydrodynamic and structure determination to elucidate larger and more flexible systems. This has an obvious link with other techniques such as electron and atomic force microscopies, as well as protein crystallography, spectroscopy and NMR structural methods.