Masaki Kinoshita

Contact

• workRoom B206 South Building
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• Masaki.Kinoshita@nottingham.ac.uk

Research Summary

Pluripotency in mammalian embryos and in culture

My research interest is pluripotent cell state in early embryos and captured ones in vitro. Pluripotency exists in early mammalian embryos but transiently. This period is further dissected into three consecutive phases termed, "naive", "formative" and "primed". These three phases are continuum but functionally distinct. We want to understand how cells transit through these phases, how they are captured molecularly and what kinds of gene regulatory network and epigenetic modification provide the functional differences. We will address the mechanisms of pluripotency and stem cell culture including mouse, livestock and human.

Recent Publications

• KINOSHITA, M., BARBER, M., MANSFIELD, W., CUI, Y., SPINDLOW, D., STIRPARO, G. G., DIETMANN, S., NICHOLS, J. and SMITH, A., 2021. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency: Cell Stem Cell Cell stem cell. 28(3), 453-471 e8
• KINOSHITA, MASAKI, KOBAYASHI, TOSHIHIRO, PLANELLS, BENJAMIN, KLISCH, DORIS, SPINDLOW, DANIEL, MASAKI, HIDEKI, BORNELÖV, SUSANNE, STIRPARO, GIULIANO GIUSEPPE, MATSUNARI, HITOMI, UCHIKURA, AYUKO, LAMAS-TORANZO, ISMAEL, NICHOLS, JENNIFER, NAKAUCHI, HIROMITSU, NAGASHIMA, HIROSHI, ALBERIO, RAMIRO and SMITH, AUSTIN, 2021. Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species: Development Development. 148(23),
• KINOSHITA, M., LI, M. A., BARBER, M., MANSFIELD, W., DIETMANN, S. and SMITH, A., 2021. Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory: Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America. 118(38),
• BURGOLD, T., BARBER, M., KLOET, S., CRAMARD, J., GHARBI, S., FLOYD, R., KINOSHITA, M., RALSER, M., VERMEULEN, M., REYNOLDS, N., DIETMANN, S. and HENDRICH, B., 2019. The Nucleosome Remodelling and Deacetylation complex suppresses transcriptional noise during lineage commitment: EMBO J The EMBO journal.

• View all publications

• KINOSHITA, M., BARBER, M., MANSFIELD, W., CUI, Y., SPINDLOW, D., STIRPARO, G. G., DIETMANN, S., NICHOLS, J. and SMITH, A., 2021. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency: Cell Stem Cell Cell stem cell. 28(3), 453-471 e8
• KINOSHITA, MASAKI, KOBAYASHI, TOSHIHIRO, PLANELLS, BENJAMIN, KLISCH, DORIS, SPINDLOW, DANIEL, MASAKI, HIDEKI, BORNELÖV, SUSANNE, STIRPARO, GIULIANO GIUSEPPE, MATSUNARI, HITOMI, UCHIKURA, AYUKO, LAMAS-TORANZO, ISMAEL, NICHOLS, JENNIFER, NAKAUCHI, HIROMITSU, NAGASHIMA, HIROSHI, ALBERIO, RAMIRO and SMITH, AUSTIN, 2021. Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species: Development Development. 148(23),
• KINOSHITA, M., LI, M. A., BARBER, M., MANSFIELD, W., DIETMANN, S. and SMITH, A., 2021. Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory: Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America. 118(38),
• BURGOLD, T., BARBER, M., KLOET, S., CRAMARD, J., GHARBI, S., FLOYD, R., KINOSHITA, M., RALSER, M., VERMEULEN, M., REYNOLDS, N., DIETMANN, S. and HENDRICH, B., 2019. The Nucleosome Remodelling and Deacetylation complex suppresses transcriptional noise during lineage commitment: EMBO J The EMBO journal.
• STUART, H. T., STIRPARO, G. G., LOHOFF, T., BATES, L. E., KINOSHITA, M., LIM, C. Y., SOUSA, E. J., MASKALENKA, K., RADZISHEUSKAYA, A., MALCOLM, A. A., ALVES, M. R. P., LLOYD, R. L., NESTOROWA, S., HUMPHREYS, P., MANSFIELD, W., REIK, W., BERTONE, P., NICHOLS, J., GOTTGENS, B. and SILVA, J. C. R., 2019. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency: Cell Stem Cell Cell stem cell. 25(3), 388-406 e8
• KINOSHITA, MASAKI and SMITH, AUSTIN, 2018. Pluripotency Deconstructed: Development, Growth & Differentiation Dev Growth Differ.
• LI, M. A., AMARAL, P. P., CHEUNG, P., BERGMANN, J. H., KINOSHITA, M., KALKAN, T., RALSER, M., ROBSON, S., VON MEYENN, F., PARAMOR, M., YANG, F., CHEN, C., NICHOLS, J., SPECTOR, D. L., KOUZARIDES, T., HE, L. and SMITH, A., 2017. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation: Elife Elife. 6,
• KINOSHITA, M., 2015. How are pluripotent cells captured in culture?: Reprod Med Biol Reprod Med Biol. 14(3), 85-98
• KINOSHITA, M., SHIMOSATO, D., YAMANE, M. and NIWA, H., 2015. Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells: BMC Dev Biol BMC developmental biology. 15(1), 37
• KINOSHITA, M., ERA, T., JAKT, L. M. and NISHIKAWA, S., 2009. The novel protein kinase Vlk is essential for stromal function of mesenchymal cells: Development Development. 136(12), 2069-79
• TADA, S., ERA, T., FURUSAWA, C., SAKURAI, H., NISHIKAWA, S., KINOSHITA, M., NAKAO, K. and CHIBA, T., 2005. Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture: Development Development. 132(19), 4363-74