Discovery of novel gene to aid breeding of climate resilient crops

Friday, 17 March 2023

Researchers have revealed for the first time how a key gene in plants allows them to use their energy more efficiently, enabling them to grow more roots and capture more water and nutrients.

An international team of plant scientists led by Penn State University and in collaboration with the University of Nottingham have discovered this novel regulatory gene (called bHLH121) that enables corn roots to acquire more water and nutrients. The findings have been published in the Proceedings of the National Academy of Science.

The gene controls the formation of air spaces among living root tissues (termed root cortical aerenchyma). Replacing a large percentage of root cells with airspaces saves the plant a lot of energy which is otherwise required to feed all these root cells. This makes roots metabolically more efficient, enabling them to use the resources saved to build more roots and explore the soil more effectively and capture more water and nutrients.

This discovery could lead to the breeding of crops that can withstand drought and low-nitrogen soil conditions and ultimately ease global food insecurity, the researchers suggest.

Identifying this gene and how it works will enable us to create more resilient crops that can withstand water and nutrient stress conditions being experienced as a result of climate change.
Rahul Bhosale, Assistant Professor in Crop Functional Genomics from the School of Biosciences and BBSRC Discovery Fellow

The research team used powerful imaging tools developed in previous research at Penn State that rapidly measured cells in thousands of roots. An imaging technique called Laser Ablation Tomography was critical for this approach. This state-of-the-art approach is also now available at the University of Nottingham through BBSRC Alert Funding and support from US partners.

Hannah Schneider, Assistant Professor of Crop Physiology at Wageningen University & Research, Netherlands said: “We first performed the field experiments that went into this study starting in 2010, growing more than 500 lines of corn at sites in Pennsylvania, Arizona, Wisconsin and South Africa,” she said. “I worked at all those locations. We saw convincing evidence that we had located a gene associated with root cortical aerenchyma.

This research revealed that mutant corn lines lacking the bHLH121 gene showed reduced root air space formation. In contrast, overexpressing bHLH121 caused more air space formation.

Characterization of these lines under suboptimal water and nitrogen availability in multiple locations revealed that the bHLH121 gene is required for root air space formation and provides a new tool for plant breeders to select varieties with improved soil exploration, and thus yield, under suboptimal conditions.

These findings are the result of many people at Penn State and beyond collaborating with us, working over many years,” he said. “We discovered the function of the aerenchyma trait and then the gene associated with it, And, it came about because of technologies that have been devised here at Penn State, such as Shovelomics — digging up roots in the field — Laser Ablation Tomography and Anatomics Pipeline. We put all those together in this work
Professor Jonathan Lynch, research lead at Penn State

The results are significant, Lynch continued, because finding a gene behind an important trait that's going to help plants have better drought tolerance and better nitrogen and phosphorus capture looms large in the face of climate change.

“Those are super important qualities — both here in the U.S. and around the world,” he said. “Droughts are the biggest risk to corn growers and are worsening with climate change, and nitrogen is the biggest cost of growing corn, from both a financial and environmental perspective. Breeding corn lines more efficient at scavenging for the nutrient would be a major development.”

The U.S. Department of Energy, the Howard G Buffett Foundation, and the U.S. Department of Agriculture’s National Institute of Food and Agriculture supported this research.


Main image credit: Hannah Schneider, Assistant Professor of Crop Physiology at Wageningen University

Story credits

More information is available from Rahul Bhosale on

Jane Icke - Media Relations Manager Science
Phone: 0115 7486462

Notes to editors:

About the University of Nottingham

Ranked 32 in Europe and 16th in the UK by the QS World University Rankings: Europe 2024, the University of Nottingham is a founding member of the Russell Group of research-intensive universities. Studying at the University of Nottingham is a life-changing experience, and we pride ourselves on unlocking the potential of our students. We have a pioneering spirit, expressed in the vision of our founder Sir Jesse Boot, which has seen us lead the way in establishing campuses in China and Malaysia - part of a globally connected network of education, research and industrial engagement.

Nottingham was crowned Sports University of the Year by The Times and Sunday Times Good University Guide 2024 – the third time it has been given the honour since 2018 – and by the Daily Mail University Guide 2024.

The university is among the best universities in the UK for the strength of our research, positioned seventh for research power in the UK according to REF 2021. The birthplace of discoveries such as MRI and ibuprofen, our innovations transform lives and tackle global problems such as sustainable food supplies, ending modern slavery, developing greener transport, and reducing reliance on fossil fuels.

The university is a major employer and industry partner - locally and globally - and our graduates are the second most targeted by the UK's top employers, according to The Graduate Market in 2022 report by High Fliers Research.

We lead the Universities for Nottingham initiative, in partnership with Nottingham Trent University, a pioneering collaboration between the city’s two world-class institutions to improve levels of prosperity, opportunity, sustainability, health and wellbeing for residents in the city and region we are proud to call home.

More news…

Media Relations - External Relations

The University of Nottingham
YANG Fujia Building
Jubilee Campus
Wollaton Road
Nottingham, NG8 1BB

telephone: +44 (0) 115 951 5798