Welcome to Deep Seq
The sequencing facility offers research scientists access to state of the art high-throughput sequencing technology.
Our experienced team of dedicated experimental scientists and bioinformaticians will work with you from the conception of your sequencing experiment to the final analysis of your data. As well as running standard sequencing projects such as RNASeq and Resequencing we provide bespoke analyses and enjoy the challenges of developing new and emerging techniques and pipelines.
Contact us to discuss your sequencing project and arrange a quote.
Sequencing Platforms
Deep Seq currently house the Illumina MiSeq and NextSeq500 sequencing platforms for low and medium throughput, which can supply single or paired reads from 75 to 600 cycles.
DeepSeq also run the Oxford NanoPore Technologies GridION and PromethION platforms and support staff running MinIONs. Deep Seq has also helped to develop software tools for MinION data analysis.
DeepSeq is also the UK sole supplier for the Bionano Saphyr, an optical mapping platform which can aid de novo assemblies and resolve complex structural variations.
For larger projects Deep Seq can outsource to high throughput sequencing suppliers and can still offer support for those projects with experimental design, library prep and bioinformatics as required.
Illumina NextSeq500
Mid throughput sequencer with up to 150bp paired-end read length
Illumina MiSeq
Low throughput sequencer up to 300bp paired-end read length
Oxford NanoPore Technologies MinION
MinIONs for long read and real-time selective sequencing
Oxford Nanopore Technologies PromethION
For high throughput long read sequencing
User Accessible Equipment TapeStation and Bioanalyser
Agilent TapeStation and Bioanalyser for sample QC
User Accessible Equipment BluePippin
Sage Science BluePippin for sample size selection
Oxford Nanopore Technologies GridION
Runs up to 5 MinIONs and basecalls data
10x Genomics Chromium
Single cell, linked-read, immune profiling library preps
Bionano Genomics Saphyr II
Optical mapping for de novo assemblies and SV detection
TTPLabtech Mosquito Hv
Liquid handling robotics for low volume pipetting
DeepSeq also houses the Sanger DNA Sequencing Lab run by Matt Carlile who has over 10 years experience in sanger sequencing techniques.
Contacts
For general Next Generation Sequencing enquiries or quotes please contact : deep.seq@nottingham.ac.uk
Events
New Long Read Plasmid Sequencing
Rapid long read whole genome plasmid analysis with Epi2Me workflows. Email us for more details.
Oxford Nanopore Technologies, Tuesday 17th October
Seminars on Nottingham research using long read sequencing and adaptive sampling with ONT platform