MEASURE (in vitro)
Bacterial surface interaction and biofilm development
The in vitro models developed as part of this work package will allow us to extract detailed molecular and cellular information on the interactions of cells with material surfaces, with the aim to enable prediction and interpretation of in vivo performance. The ChemoTopoChips with separated cell culture environments produced in MAKE will enable us to use image-based cell response quantification complemented by biomolecular characterisation of the host and bacterial cell secretome. Bacterial surface interaction and biofilm development will be correlated with material properties and surface analysis data to inform optimal infection and immune responses in bacterial/immune cell/fibroblast co-culture models. This will be used to guide the design process and extended to macrophage and fibroblast structure-function relationships in MODEL.