MEASURE (in vivo)
Performance of selected materials in mouse models
To characterise the performance of selected material chemistry-topography implants in an environment relevant to the human immune response, we will compare wound healing and foreign body responses in un-infected and infected conditions in sub-cutaneous foreign body implants and full thickness skin wound mouse models. Longitudinal non-invasive study of infection using established optical in vivo imaging will be undertaken for a range of topographically patterned polymers. At appropriate end points, tissues and implants will be harvested for immunohistochemical staining, high content confocal fluorescent microscopy imaging, and flow cytometry. This data will be fed to MODEL to be linked to detailed cell/pathogen/biomolecular data from MEASURE (in vitro).