ISAC's Horiba LabRam HR - A  multifunctional Raman Spectrometer

Raman Spectroscopy

The HORIBA LabRAM HR confocal Raman microscope available at the nmRC

Raman Spectroscopy at a glance

Raman spectroscopy is a non-invasive optical technique used to determine the chemical identity and state of a sample by its unique vibrational (molecular) modes. Inelastic (loss or gain of energy) scattering of light from the sample is measured, with distinct shifts in the energy of the scattered photons being representative of the sample chemistry.


  • Vibrational spectra of substrate chemistry
  • Confocal component mapping and depth profiling
  • UV-Visible-IR excitation analysis
  • Temperature and time dependent chemical and physical state change identification  
  • Ambient or controlled environment analysis
 ISAC's Horiba LabRam HR - Automated sample raster for mapping proceedures

How does Raman work?

Raman spectroscopy and microscopy is a uniquely sensitive analytical technique that is non-invasive in nature. It can rapidly provide quantifiable identification of bulk and surface species with exceptional resolution. The technique measures the interaction between a monochromatic light source and the vibrational (and other low-frequency) modes of the molecular systems being analysed.

A given molecular vibration can be excited by a certain frequency of incident light to a higher virtual energy state. This higher energy state will almost always relax again to a lower energy state. While normally this occurs elastically (to its original energy state as Rayleigh scattering) it may also occur inelastically. This represents relaxation to a different vibrational energy level, generating the release of a photon with an altered frequency to that of the incident photon.

This change in frequency is the Raman shift, and can be recorded and plotted against the scattered light intensity. Therefore Raman scattering gives rise to reductions or gains in light frequencies that correspond very sensitively to the molecular structure and state of the material being analysed. Solving these spectra allows characterisation of the chemical and physical state of a material.



Our Raman Spectroscopy Facilities


ISAC's Horiba LabRam HR - Variable illumination sources and objective lenses
ISAC's Horiba LabRam HR - Automated Sample Mapping



  • Four excitation wavelengths available:
    1. Kimmon IK3201R-F He-Cd laser (λ = 325 nm, average power = 20 mW)
    2. Laser Quantum Torus 532 100mW laser (λ = 532 nm, average power = 129 mW)
    3. Laser Quantum Ignis 660 100mW (λ = 660 nm, average power = 187 mW)
    4. Toptica Photonics XTRA-PS (λ = 785 nm, average power = 385 mW)
  • Choice of standard CCD (HORIBA Synapse) or EM-CCD (Andor Technology Newton 970).
  • Olympus BX41 stage with x40 (UV only), x10 and x50 (visible and NIR) objective lenses.
  • Linkam Scientific TMS 94 system (thermal stage) capable of operating between -196 and 350 ˚C.
  • SWIFT® mode offers confocal Raman mapping with CCD integration times down to 1 ms per point and below.
  • DuoScan® mode is a confocal imaging mode, with high precision, ultra-fast rastering mirrors creating variable sized laser macro-spots, and also allowing nano-step mapping from deep UV to NIR.
  • Choice of gratings for standard or high resolution spectroscopies.



HORIBA XploRA INV Inverted Raman Microscope

  • Inverted microscope geometry suitable for 'in-situ' analysis and biological samples.
  • Excitation wavelength available: 785nm.
  • Gratings available: 600, 1200, 1800 and 2400 lines/mm (configuration dependent).
  • Objectives available: 10x, 20x and 100x.


Publications of Interest

Nanoscale and Microscale Research Centre

Cripps South building
University of Nottingham
University Park
Nottingham, NG7 2RD

telephone: +44 (0) 115 95 15046
email: nmcs@nottingham.ac.uk