Biological sample preparation for chromatography including liquid and solid phase extraction, centrifugation, rotary evaporation and speedvac concentration are available. The analytical laboratory houses high pressure liquid chromatography equipment with autosampling linked to UV, diode array and refractive index detectors as well as a Bruker ion trap mass spectrometer with electrospray and chemical ionisation.
Contact Nigel Halliday
Specialist expertise in monoclonal antibody production to a range of antigens including glycoproteins and lipids, chimerisation, humanisation, Fc-optimisation, purification (and quality control). This includes tailored antibody development to unique antigens, idiotype generation and antibody cloning, chimerisation and humanisation.
Contact Lindy Durrant
A Gulmay Medical Cabinet provides an opportunity to conduct radiation biology experiments in vitro and in vivo. The enclosed system incorporates a 225kV broad focal X-ray housed in a fully equipped tissue culture laboratory. Atmospheric control allows O2 concentrations of down to 0.2% to be maintained for hypoxic studies.
Contact Stewart Martin
The Oncology Research Group in partnership with the NHS Department of Clinical Oncology and the Mid Trent National Cancer Network has a state of the art Cancer Clinical trials team. The group is a 30 person strong multidisciplinary team of research nurses and radiographers, data managers and ethics and finance managers and carries out both simple and complex clinical trials according the highest standards of Good Clinical Practice.
Contact Poulam Patel
A combination of single and twin BIOSTAT B 1L and 2L fermentors are available for pilot and ‘omics bacterial’ growth experiments.
All growth parameters are computer controlled via SCADA Software (MFCS/DA, B. Braun Biotech International) enabling data acquisition, process visualisation and control as well as evaluation and data export.
Contact Miguel Cámara and Nigel Minton
Fluorescence activated cell sorters (BeckmanCoulter Altra and MoFlo XDP) and an analysis only cytometer (Beckman Coulter FC500) are available.
Contact Adrian Robins
Genomics facility (CBS Building)
Dedicated to microarray and real time qRT-PCR analysis. Instrumentation available includes an Agilent DNA Hybridisation Station, an Axon 4000B microarray and access to GeneSpring GX analysis software. For qRT-PCR, an Applied Biosystems AB7500 permits relative quantification, absolute quantification, allelic discrimination, plus/minus assays and basic melt curve analysis. In addition, a Roche Lightcycler 480, offers high resolution melt analysis.
Contact Victoria Wright
Post-genomics facility (QMC campus)
This facility offers fluorescent probe generation and large scale microarraying of DNA, protein (Including Reverse-phase protein arrays) and other biomolecules (Genomic solutions Microgrid II 600 robot with both steel and silicon split pin configurations). Quality control support is available (Nanodrop Spectrophotometer and Agilent Bioanalyser), along with customisable slide surface chemistries and automated sample hybridisation capabilities (Tecan HS4800 24 place station). Analysis of both fluorescently and colorimetricaly labelled microarrays is available with a four laser automated slide scanner (Genepix 4200AL) and high resolution white light scanner.
Contact Paddy Tighe
Light and Fluorescence Microscopy
These include a Zeiss LSM 700 laser scanning confocal with an Axio Observer inverted microscope, a Nikon Eclipse TE200 and a fluorescent/phase contrast Nikon Labphot-2 microscope fitted with digital cameras.
Contact Steve Atkinson
Includes: tissue and cell banking, basic tissue preparation (processing, section cutting and staining), immunocytochemistry, laser micro-dissection, tissue microarrays, digital pathology.
Contact Andy Green
Protein purification and X-ray crystallography
Protein purification using an automated AKTA system employing a range of solid phases, Protein samples are used to set up 96 well plate crystallisation screens using a Hydra II robotic system. The crystals obtained are subjected to X-rays generated by a Rigaku RAXIS IV++ X-ray area detector to produce diffraction data.
Contact Chris Penfold
Animal Imaging Facility
Specialist expertise in pre-clinical imaging of model organisms, using a state of the art facility run under the reduction, replacement and refinement ethos. The facility contains a Mediso Nano-pet/ct, Mediso Nano SPECT/CT and an optical Ivis Spectrum. Also available within this facility is a dedicated Radio-chemist for radio-nuclide labelling of novel compounds.
The Greenfield Medical Library
The University library service provides access to more than a million books and journals.
The Greenfield Medical Library houses a broadly based collection of biomedical, nursing and healthcare related books and periodicals and holds current subscriptions to 780 journals, reports and series titles. In addition to the print versions housed in the library, the majority of journals can be accessed electronically.