nmRC
Nanoscale and Microscale Research Centre
   
   
  

Secondary Ion Mass Spectrometry

OrbiSIMS image

3D OrbiSIMS  Facility

 

3D OrbiSIMS

A Time of Flight Secondary Ion Mass Spectrometer (ToF-SIMS) with hybrid OrbiTrapTM functionality, the '3D OrbiSIMS' is the first of its kind in an academic setting. Based on the prototype at the National Physical Laboratory (NPL) the instrument combines the function of the two hybridised components to facilitate an unprecedented level of mass spectral molecular analysis for a range of materials (hard and soft matter, biological cells and tissues). The dual functionality of this hybrid system underpins spatial and mass resolution improvements in chemical identification and imaging that revolutionize mass spectrometry imaging. Most notably the capacity to identify unknown organic species in complex solid samples is dramatically improved. Importantly, the facility is complemented by high pressure freezing cryo-preparation facilities that enable biological samples to be maintained close to their native state. Complemented by the more commonly applied (but more disruptive) freeze drying and sample fixation the 3D OrbiSIMS facility is ideally targeted at soft matter analyses and enables label free molecular analysis. However when the exceptional surface sensitivity (1-3nm) and high mass/spatial resolution are combined with a depth profiling sputtering beam, the instrument becomes an extremely powerful tool for 3D chemical analysis on a multitude of sample types.

Key Features

  • High mass resolution spectrometry (>240,000 and 11,000 amu for the OrbiTrap and the ToF, respectively.
  • High spatial resolution chemical imaging (<70 nm).
  • Gas cluster ion beam sputtering for controlled depth profile analysis of organics.
  • Cryogenic sample preparation facility, including high pressure freezing, freeze drying, cryo-ultramicrotomy and more.
  • Category 2 preparation facilities for cell/tissue analysis.

 

 

 

Research Highlights 

3D MS imaging using dual beam and dual spectrometer (mode 10) of single rat alveolar macrophage cell incubated in media with the drug amiodarone

 Orbi_Nature_Cropped

(a) Sequence of 30 keV Bi3+ (~300-nm resolution) ToF-SIMS images of total ion counts at ~400-nm depths as the cell is sputtered away. (b) As in a, color overlay of phosphocholine marker (m/z 184), gray, nuclear marker (m/z 157), magenta and amiodarone [M+H]+ (m/z 646), green (c) 3D rendering of the cell with phosphocholine marker, (m/z 184, gray, opacity 0.40), nuclear marker (m/z 157, magenta) and amiodarone [M+H]+ (m/z 646), green.

The 3D OrbiSIMS - Label-Free Metabolic Imaging with Sub-cellular Lateral Resolution and High Mass Resolving Power, Passarelli et al., Nature Methods, DOI 10.1038/nmeth.4504

 

 

 

 

 

 

Nanoscale and Microscale Research Centre

Cripps South building
University of Nottingham
University Park
Nottingham, NG7 2RD

telephone: +44 (0) 115 748 6340
email: nmrcenquiries@nottingham.ac.uk