School of Life Sciences
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Image Analysis

Image Analysis 

Software

There is a range of equipment for image analysis, deconvolution, tracking and colocalisation:

  • Volocity is imaging software used with 2D and 3D images. It has many features including: colocalisation, deconvolution (‘fast’ to improve an image for presentation and ‘iterative’ to sharpen an image for analysis) and movie sequence capabilities. There are also many other options including: collating and viewing data graphically, creating 3D representations of data and tracking specific objects through a time sequence.
  • Huygens Professional is tailored for deconvolution/restoration of microscopic images. Our version also has modules for advanced object finding/tracking, 3D rendering, chromatic aberration correction and colocalisation.
  •  Fiji is an image processing package. It can be described as a "batteries-included" distribution of ImageJ (and ImageJ2), bundling Java, Java3D and a lot of plugins organized into a coherent menu structure. It is freely available.

Applications

  • Deconvolution of data stacks.
  • Measurement of colocalisation.
  • Quantification of areas, volumes and regions of interest.
  • Cell/molecule tracking in 3D and 4D.
  • Image analysis and quantification of areas, volumes, ROIs, lengths, thresholding, object size and distribution, and 3D image rendering and animation.

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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