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Stephen E. Harding

Professor of Applied Biochemistry, Faculty of Science

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Biography

Born: Wallasey, Wirral; Education: Pembroke College Oxford. Qualifications: BA, MA, DSc (Oxon). Employment: Lister PostDoc, Univ Bristol (1981-2); Oppenheimer Postdoc, Univ Cambridge (1983-4); Supervisor (Queens College Cambridge 1984); Univ Nottingham (84-present). Visiting Prof: Friedrich Schiller Univ, Jena, (2009-13). Adjunct Prof: Univ Oslo (2017-present). Awards: RSC Junior Medallist (1991); Oxford Univ DSc (2002); Ridder Første den Kongelige Norske Fortjenstorden (2012); 2016 Hakon Hakonsson Lecturer; 2017 Svedberg Lecturer; Wikipedia Norway - Persona fra Wallasey; Knights of the Royal Norwegian Order of Merit. Honorary Member, British Biophysical Society (2020-present) Google Scholar H index = 66; Publication downloads & links (Research Gate); Books (Amazon.co.uk); Orcid (Research Club).

Course Director: MRes in Biomolecular Technology (Now accepting students for 2022 entry)

Research Summary

The NCMH is involved in developing & applying hydrodynamic methodology for shape, size and interaction analysis of macromolecules in solution. 1. Shape: we have championed the "whole-body"… read more

Selected Publications

Current Research

The NCMH is involved in developing & applying hydrodynamic methodology for shape, size and interaction analysis of macromolecules in solution. 1. Shape: we have championed the "whole-body" (ellipsoid) approach for representing the overall shape of proteins, developing the combined-function approach (usually viscosity + one other parameter) to eliminate hydration effects, & extending to tri-axial ellipsoids, complementing the "bead" approach pioneered by Garcia de la Torre. We developed ways of dealing with concentration dependent non-ideality (co-exclusion & repulsion), working with Rallison, Winzor & Janet Thornton, & paving the way for dealing with high concentration systems (Harding & Johnson) used in e.g. monoclonal antibody formulations. For glycan shape we have worked with Garcia de la Torre on HYDFIT, which evaluates flexibility by combining different hydrodynamic measurements together. 2. Size: Our MSTAR method from sedimentation equilibrium, gives accurate mol wt averages even for highly polydisperse systems and systems that float (we were the first to develop flotation equilibrium analysis) & our Extended Fujita method combines this with sedimentation velocity to give mol wt distributions: all without the complications of a separation matrix. 3. Interactions - aminocelluloses. Using diagnostic plots & our algorithm MULTISIG it's possible to distinguish molecular polydispersity from self-association, enabling elucidation of very weak interactions in polydisperse systems like xyloglucans and amazing protein-like associative behaviour of amino-celluloses, a feature now proving invaluable in the development of new consolidants to replace/reinforce rotted cellulose in badly degraded archaeological wood, a significant part of our Saving Oseberg project with Oslo.

Text-books: Our work features strongly in e.g. Methods in Molecular Biophysics (Seryduk et al, CUP 2007) & Wilson & Walker's Principles & Techniques of Biochemistry & Molecular Biology (CUP 2018). A book has been commissioned for 2021 by the Royal Society of Chemistry: Biomolecular Hydrodynamics & Rheology (Harding & Yakubov)

Current Funding: 1. GSK Vaccines (RIS/360533): Hydrodynamics & stability of GBS polysaccharides & CRM197 protein. £0.12M: Harding, Adams, Fisk (1/6/2018 - 31/05/2021). 2. EPSRC (EP/S021434/1): High resolution, cryogenic analytical & transfer scanning electron microscope (HR-CAT-SEM). £1.95M: Khlobystov, Alexander, Scurr, Brown, Grant, Snape, Wright, Amabilino, Howdle, Parmenter, Rawson, Vandeginste, Harding & Arkill (1/2019 - 12/2024). 3. BBSRC (BB/T006404/1): Biophysical defence in the mammalian gut: Unlocking the molecular mechanisms of dietary fibre interaction with mucin glycoproteins. £0.46M: Yakubov & Harding (10/2020 - 9/2023). Under consideration: 1. EPSRC (EP/V011731/1): Bioinspired polymer consolidants for saving & sustaining archaeological wood (B-SAW). £0.67M: Harding, Stockman, Irvine, Howdle, Watson, Yakubov, Phillips-Jones & Schofield (1/2021 - 12/2023).2. NFR (Norway): Virtual reconstruction, interpretation & preservation of the textile artefacts from the Oseberg find (TEXREC) Kutzke, Vedeler, Hardeberg, George, Harding, Herrmann & Geiger (6/2021 - 5/2025)

School of Biosciences

University of Nottingham
Sutton Bonington Campus
Nr Loughborough
LE12 5RD, UK

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