Recent Publications

Healthcare

High-resolution mass spectrometry imaging reveals metabolic signatures of Staphylococcus epidermidis in host defense against influenza A virus, Kim K.-S., Wong K. F. C., Jo A., Martínez-Jarquín S., Jha R., Kotowska A. M., Kim H. J., Kim D.-H., Cho J.-Y., Analytica Chimica Acta, 1381, 344803, 2026.

Surface lipids in nematodes are influenced by development and species-specific adaptations, Kotowska A. M., Hiramatsu F., Alexander M. R., Scurr D. J., Lightfoot J. W., Chauhan V. M., Journal of the American Chemical Society, 147, 8, 6439-6449, 2025.

Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants, Crucitti, V. C., Hajiali H., Dundas A. A., Jayawarna V., Tomolillo D., Francolini I., Vuotto C., Salmeron-Sanchez M., Dalby M. J., Alexander M. R., Wildman R. D., Rose F. R. A. J., Irvine D. J., Journals of Materials Chemistry B, 13, 15, 4657-4670, 2025.

Study on molecular orientation and stratification in RNA-lipid nanoparticles by cryogenic orbitrap secondary ion mass spectrometry, Kotowska A. M., Fay M., Watts, J. A., Gilmore, I. S., Scurr D. J., Howe A., Capka V., Perez C. E., Doud D., Patel S., Communications Chemistry, 8, 1, 160, 2025.

Combinatorial discovery of microtopographical landscapes that resist biofilm formation through quorum sensing mediated autolubrication, Romero M., Luckett J., Dubern J.-F., Figueredo G. P., Ison E., Carabelli A. M., Scurr D. J., Hook A. L., Kammerling L., da Silva A. C., Nature Communications, 16, 1, 5295, 2025.

Metabolomic and proteomic analysis of ApoE4-carrying H4 neuroglioma cells in Alzheimer’s disease using OrbiSIMS and LC-MS/MS, Li Lu, Anna M Kotowska, Stefanie Kern, Min Fang, Timothy R Rudd, Morgan R Alexander, David J Scurr, Zheying Zhu Anal. Chem., 96, 11760–11770, 2024.

Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids, Suvannapruk W., Fisher L. E., Luckett J. C., Edney M. K., Kotowska A. M., Kim D.-H., Scurr D. J., Ghaemmaghami A. M., Alexander M. R., Advanced Science, 2306000, 2024.

Targeted intradermal delivery of alpha-arbutin-loaded dissolving polymeric microneedles visualized by three-dimensional Orbitrap secondary ion mass spectrometry (3D OrbiSIMS), Li Z., Marlow M., Scurr D., Zhu Z., European Journal of Pharmaceutics and Biopharmaceutics, 196, 114181, 2024.

Toward Comprehensive Analysis of the 3D Chemistry of Pseudomonas aeruginosa Biofilms, Kotowska A. M., Zhang J., Carabelli A., Watts J., Aylott J. W., Gilmore I. S., Williams P., Scurr D. J., Alexander M. R., Anal. Chem. 95, 49, 18287-18294, 2023.

Investigating ApoE4-mediated metabolic alteration of H4 neuroglioma cells of Alzheimer's disease, Lu L., Kotowska A., Kern S., Fang M., Rudd T. R., Alexander M., Scurr D., Zhu Z., Alzheimer's & Dementia, 19, S24, e082458, 2023.

Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation, Majrashi M., Kotowska A., Scurr D., Hicks J. M., Ghaemmaghami A., Yang J., ACS Appl. Mater. Interfaces, 15, 49, 56623-56638, 2023.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents, Brookes A., Kindon N., Scurr D. J., Alexander M. R., Gershkovich P., Bradshaw T. D., BJC Reports, 2023.

Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo using 3D OrbiSIMS, Suvannapruk W., Edney M. K., Fisher L.E., Luckett J. C., Kim D.-H., Scurr D. J., Ghaemmaghami A. M., Alexander M. R., Bio-protocol, 13, 15, 2023.

Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS, Khateb H., Hook A. L., Kern S., Watts J. A., Singh S., Jackson D., Marinez-Pomares L., Williams P., Alexander M. R., Biointerphases, 18(3), 031007, 2023.

Untargeted Metabolomic Characterization of Glioblastoma Intra-Tumor Heterogeneity Using OrbiSIMS, He W., Edney M. K., Paine S. M. L., Griffiths R. L., Scurr D. J., Rahman R., Kim D.-H., Anal.Chem. 95, 14, 5994-6001, 2023.

Design, Synthesis, and Biological Evaluation of Novel Hybrids Containing Dihydrochalcone as Tyrosinase Inhibitors to Treat Skin Hyperpigmentation, Xue S., Li Z., Ze X., Wu X., He C., Shuai W., Marlow M., Chen J., Scurr D., Zhu Z., Xu J., Xu S., J. Med. Chem., 66, 7, 5099-5117, 2023.

Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site, Muresan P., McCrorie P., Smith F., Vasey C., Taresco V., Scurr D. J., Kern S., Smith S., Gershkovich P., Rahman R., Marlow M., European Journal of Pharmaceutics and Biopharmaceutics, 182, 53-61, 2023.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging, Linke F., Johnson J. E. C., Kern S., Bennett C. D., Lourdusamy A., Lea D., Clifford S. C., Merry C. L. R., Stolnik S., Alexander M. R., Peet A. C., Scurr D. J., Griffiths R. L., Grabowska A. M., Kerr I. D., Coyle B., Acta Neuropathologica Communications, 11, 6, 2023.

Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype, Suvannapruk W., Edney M. K., Kim D.-H., Scurr D. J., Ghaemmaghami A. M., Alexander M. R., Analytical Chemistry, 94, 26, 9389-9398, 2022.

Elucidating the molecular landscape of the stratum corneum, Starr N. J., Khan M. H., Edney M. K., Scurr D. J., PNAS, 119 (12) e2114380119, 2022.

Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets, Edney M. K., Kotowska A. M., Spanu M., Trindade G. F., Wilmot E., Reid J., Barker J., Aylott J. W., Shard A. G., Alexander M. R., Snape C. E., Scurr D. J., Analytical Chemistry, 94, 11, 4703-4711, 2022.

Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens, Watson R., Oldfield M., Bryant J. A., Riordan L., Hill H. J., Watts J. A., Alexander M. R., Cox M. J., Stamataki Z., Scurr D. J., de Cogan F., Scientific Reports, 12, 2803, 2022.

Developing Novel Biointerfaces: Using Chlorhexidine Surface Attachment as a Method for Creating Anti-Fungal Surfaces, Bryant J. A., Riordan L., Watson R., Nikoi N. D., Trzaska W., Slope L., Tibbatts C., Alexander M. R., Scurr D. J., May R. C., de Cogan F., Global Challenges, 2100138, 2022.

Detection of Label-Free Drugs within Brain Tissue using Orbitrap Secondary Ion Mass Spectrometry as a Complement to Neuro-Oncological Drug Delivery, McCrorie P., Rowlinson J., Scurr D. J., Marlow M., Rahman R., Pharmaceutics, 14(3), 571, 2022.

Sequential Orbitrap Secondary Ion Mass Spectrometry and Liquid Extraction Surface Analysis-Tandem Mass Spectrometry-Based Metabolomics for Prediction of Brain Tumor Relapse from Sample-Limited Primary Tissue Archives, Meurs J., Scurr D. J., Lourdusamy A., Storer L. C. D., Grundy R. G., Alexander M. R., Rahman R., Kim D.-H., Analytical Chemistry, 2021.

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells, Hodgkinson T., Tsimbouri P. M., Llopis-Hernandes V., Campsie P., Scurr D., Childs P. G., Phillips D., Donnelly S., Wells J. A., O'Brien F. J., Salmeron-Sanchez M., Burgess K., Alexander M., Vassalli M., Oreffo R. O. C., Reid S., France D. J., Dalby M. J., Science Advances, 7(9), eabb7921, 2021.

Protein Identification by 3D OrbiSIMS to Facilitate in situ Imaging and Depth Profiling, Kotowska A. M., Trindade G. F., Mendes P. M., Williams P. M., Aylott J. W., Shard A. G., Alexander M. R., Scurr D. J., Nature Communications, 11(5832), 2020

Immune Modulation by Design: Using Topography to Control Human Monocyte Attachment and Macrophage Differentiation, Vassey M. J., Figueredo G. P., Scurr D. J., Vasilevich A. S., Vermeulen S., Carlier A., Luckett J., Beijer N. R. M., Williams P., Winkler D. A., de Boer J., Ghaemmaghami A. M., Alexander M. R., Advanced Science, 7(11), 2020

Cryo-OrbiSIMS for 3D Molecular Imaging of a Bacterial Biofilm in its Native State, Zhang J., Brown J., Scurr D. J., Bullen A., MacLellan-Gibson K., Williams P., Alexander M. R., Hardie K. R., Gilmore I. S., Rakowska P. D., Analytical Chemistry, 92(13), 9008-9015, 2020

Analytical Method Development

High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis- Tandem Mass Spectrometry (LESA-MS/MS), Meurs J., Nasir A., Figueredo G. P., Burroughs L., Abdelrazig S. A., Denning C., Winkler D., A., Barrett D. A., Kim D.-H., Alexander M. R., Analytical Chemistry, 97, 24, 12776-12785, 2025.

Validation of the Use of ToF-SIMS for Analysis of Glycosaminoglycans, Milne L. K., D/O Ajayakumar A. A., Holyland I L., Kotowska A. M., Hogwood J., Gray E., Mulloy B., Merry C. L. R., Hook A. L., Carbohydrate Polymers, 371, 124415, 2026.

Comparison of Tiling Artifact Removal Methods in Secondary Ion Mass Spectrometry Images, Kandanaarachchi S., Gardner W., Alexander D. L. J., Muir B. W., Chouinard P. A., Crewther S. G., Scurr D. J., Halliday M., Pigram P. J., Anal. Chem., 95, 47, 17384-17391, 2023.

A New Particle Mounting Method for Surface Analysis, Dundas A. A., Kern S., Cuzzucoli Crucitti V., Scurr D. J., Wildman R., Irvine D. J., Alexander M. R., Surface and Interface Analysis, 54, 4, 374-380, 2021.

Direct Immobilization of Engineered Nanobodies on Gold Sensors, Simões B., Guedens W. J., Keene C., Kubiak-Ossowska K., Mulheran P., Kotowska A. M., Scurr D. J., Alexander M. R., Broisat A., Johnson S., Muyldermans S., Devoogdt N., Adriaensens P, Mendes P. M., ACS Applied Materials & Interfaces, 13(15), 17353-17360, 2021.

The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power, Melissa K Passarelli, Alexander Pirkl, Rudolf Moellers, Dmitry Grinfeld, Felix Kollmer, Rasmus Havelund, Carla F Newman, Peter S Marshall, Henrik Arlinghaus, Morgan R Alexander, Andy West, Stevan Horning, Ewald Niehuis, Alexander Makarov, Colin T Dollery & Ian S Gilmore Nature Methods 14, 1175–1183, 2017.

Anthropology

Mechanisms of Lipid Preservation in Archaeological Clay Ceramics Revealed by Mass Spectrometry Imaging, Hammann S., Scurr D. J., Alexander M. R., Cramp L. J. E., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 117(26), 14688-14693, 2020.

Energy and Engineering

Investigating the Failure Mechanism of Wet eMachines in the Presence of Transmission Oils, Nyman P., Björklund P., Yaghini N., Lu L., Hopper E. R., Hunt G., J. Tribol., 147(5), 051111, 2025.

Dissociative Host-Dopant Bonding Facilities Molecular Doping in Halide Perovskites, Lanzetta L., Gregori L., Huerta Hernandez L., Sharma A., Kern S., Kotowska A. M., Emwas A.-H., Gutiérrez-Arzaluz L., Scurr D. J., Piggott M., Meggiolaro D., Haque Md A., De Angelis F., Baran D., ACS Energy Lett., 8, 7, 2858-2867, 2023.

A Multifaceted Ferrocene Interlayer for Highly Stable and Efficient Lithium Doped Spiro-OMeTAD-based Perovskite Solar Cells, Webb T., Liu X., Westbrook R. J. E., Kern S., Sajjad M. T., Jenatsch S., Imalka Jayawardena K. D. G., Hashini K. Perera W., Marko I. P., Sathasivam S., Li B., Yavari M., Scurr D. J., Alexander M. R., Macdonald T. J., Haque S. A., Sweeney S. J., Zhang W., Advanced Energy Materials, 12, 26, 2200666, 2022.

Internal Diesel Injector Deposit Chemical Speciation and Quantification Using 3D OrbiSIMS and XPS Depth Profiling, Lamb J. S., Barker J., Wilmot E., Scurr D. J., Snape C. E., Smith E. F., Alexander M. R., Reid J.,SAE International Journal of Advances and Current Practices in Mobility, 3, 349-364, 2020.

The Effect of Temperature on the Molecular Compositions of External and Internal Gasoline Direct Injection Deposits, Edney M. K., Smith E. F., Wilmot E., Reid J., Barker J., Alexander M. R., Snape C. E., Scurr D. J., SAE International, 2021.

Residual Polymer Stabiliser causes Anisotropic Electrical Conductivity during Inkjet Printing of Metal Nanoparticles, Trindade G. F., Wang F., Im J., He Y., Balogh A., Scurr D., Gilmore I., Tiddia M., Saleh E., Pervan D., Turyanska L., Tuck C. J., Wildman R., Hague R., Roberts C. J., Communications Materials, 2, 47, 2021.

Spatially Resolved Molecular Compositions of Insoluble Multilayer Deposits Responsible for Increased Pollution from Internal Combustion Engines, Edney M. K., Lamb J. S., Spanu M., Smith E. F., Steer E., Wilmot E., Reid J., Barker J., Alexander M. R., Snape C. E., Scurr D. J., ACS Applied Materials & Interfaces, 12(45), 51026-51035, 2020.